Injectable Hydrated Calcium Phosphate Bone-like Paste: Synthesis, In Vitro, and In Vivo Biocompatibility Assessment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis Procedure and Characterization
2.1.1. Synthesis of hCPP
2.1.2. X-ray Diffraction Analysis
2.1.3. Infrared Spectroscopy
2.1.4. Specific Surface Area hCPP
2.1.5. Dynamic Light Scattering (DLS) and ζ Potential
2.2. In Vitro Studies
2.2.1. Cell Culture
2.2.2. Cytotoxicity Assays
2.2.3. Protein Adsorption Assay
2.3. In Vivo Studies
2.3.1. Animals
2.3.2. Surgical Subcutaneous Injection
2.3.3. Histological Analysis
2.4. Statistical Analysis
3. Results
3.1. Results of Structural and Physical–Chemical Analyses
3.2. Results of Dynamic Light Scattering (DLS) and ζ-Potential Assay
3.3. Results of Cytotoxicity Assays
3.4. Results of Protein Adsorption Assay
3.5. Results of In Vivo Biocompatibility Assays
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample-0 | HA (1250 °C) | HA (1250 °C) | α-TCP | ||||
---|---|---|---|---|---|---|---|
2Theta | (hkl) | 2Theta | (hkl) | 2Theta | (hkl) | 2Theta | (hkl) |
10.86 | 100 | 55.98 | 322 | 12.11 | 031 | ||
21.81 | 200 | 57.21 | 313 | 22.23 | 201 | ||
22.93 | 111 | 58.31 | 204 | 22.77 | –162 | ||
25.39 | 201 | 58.88 | 330 | 23.38 | –204 | ||
25.96 | 002 | 25.91 | 002 | 60.03 | 420 | 23.87 | 033 |
28.49 | 102, 210 | 28.16 | 102 | 60.49 | 331 | 24.14 | –261 |
28.99 | 210 | 61.76 | 421 | 24.31 | 043 | ||
32.07 | 211, 112 | 31.83 | 211 | 63.10 | 502 | 26.72 | –334 |
32.24 | 112 | 63.53 | 510 | 29.73 | –361 | ||
33.40 | 300, 202 | 32.97 | 300 | 64.14 | 304 | 30.38 | –191 |
34.17 | 202 | 65.18 | 511 | 30.76 | 034 | ||
35.53 | 301 | 66.51 | 422 | 31.30 | –335 | ||
39.67 | 212, 310 | 39.24 | 212 | 34.62 | 400 | ||
39.88 | 310 | 41.43 | |||||
40.48 | 221 | 41.76 | |||||
40.87 | 103 | ||||||
42.07 | 311 | ||||||
42.33 | 302 | ||||||
43.90 | 113 | ||||||
44.40 | 400 | ||||||
45.40 | 203 | ||||||
46.83 | 222 | 46.79 | 222 | ||||
48.16 | 312 | ||||||
48.71 | 320 | ||||||
49.67 | 213 | 49.56 | 213 | ||||
50.59 | 321 | ||||||
51.51 | 410 | 51.37 | 410 | ||||
52.17 | 402 | ||||||
53.26 | 004 | 53.26 | 004 |
Sample | a(Å) | c(Å) | V(Å3) |
---|---|---|---|
Card №84-1998 | 9.4166 | 6.8745 | 527.9 |
Sample_0 | 9.39(4) | 6.875(12) | 525.3(22) |
HA | 9.4062(18) | 6.8707(15) | 526.45(15) |
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Teterina, A.Y.; Minaychev, V.V.; Smirnova, P.V.; Kobiakova, M.I.; Smirnov, I.V.; Fadeev, R.S.; Egorov, A.A.; Ashmarin, A.A.; Pyatina, K.V.; Senotov, A.S.; et al. Injectable Hydrated Calcium Phosphate Bone-like Paste: Synthesis, In Vitro, and In Vivo Biocompatibility Assessment. Technologies 2023, 11, 77. https://doi.org/10.3390/technologies11030077
Teterina AY, Minaychev VV, Smirnova PV, Kobiakova MI, Smirnov IV, Fadeev RS, Egorov AA, Ashmarin AA, Pyatina KV, Senotov AS, et al. Injectable Hydrated Calcium Phosphate Bone-like Paste: Synthesis, In Vitro, and In Vivo Biocompatibility Assessment. Technologies. 2023; 11(3):77. https://doi.org/10.3390/technologies11030077
Chicago/Turabian StyleTeterina, Anastasia Yu., Vladislav V. Minaychev, Polina V. Smirnova, Margarita I. Kobiakova, Igor V. Smirnov, Roman S. Fadeev, Alexey A. Egorov, Artem A. Ashmarin, Kira V. Pyatina, Anatoliy S. Senotov, and et al. 2023. "Injectable Hydrated Calcium Phosphate Bone-like Paste: Synthesis, In Vitro, and In Vivo Biocompatibility Assessment" Technologies 11, no. 3: 77. https://doi.org/10.3390/technologies11030077