The PIFs Redundantly Control Plant Defense Response against Botrytis cinerea in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. The Effect of PIF Mutations on Resistance to B. cinerea
2.2. The Effect of PIFs in Regulating the Basal and Pathogen-Induced Expression of Defense-Related Gene
2.3. The Effect of PIF Overexpressions on Resistance to B. cinerea
2.4. Expression Profiling to Identify the Potential PIF-Involved Pathway to Control Defense Response
2.5. In Vivo Interaction between PIF4/5 and the ERF1 Promoter
2.6. Association of PIF-Regulated Defense with JA/ET Signaling
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Pathogen Infection
4.3. Expression Analysis
4.4. RNA Sequencing
4.5. Gel Mobility Shift Assay
4.6. Chromatin Immunoprecipitation Assay
4.7. Protoplast Transfection Assays
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Xiang, S.; Wu, S.; Zhang, H.; Mou, M.; Chen, Y.; Li, D.; Wang, H.; Chen, L.; Yu, D. The PIFs Redundantly Control Plant Defense Response against Botrytis cinerea in Arabidopsis. Plants 2020, 9, 1246. https://doi.org/10.3390/plants9091246
Xiang S, Wu S, Zhang H, Mou M, Chen Y, Li D, Wang H, Chen L, Yu D. The PIFs Redundantly Control Plant Defense Response against Botrytis cinerea in Arabidopsis. Plants. 2020; 9(9):1246. https://doi.org/10.3390/plants9091246
Chicago/Turabian StyleXiang, Shengyuan, Songguo Wu, Haiyan Zhang, Minghui Mou, Yanli Chen, Daibo Li, Houping Wang, Ligang Chen, and Diqiu Yu. 2020. "The PIFs Redundantly Control Plant Defense Response against Botrytis cinerea in Arabidopsis" Plants 9, no. 9: 1246. https://doi.org/10.3390/plants9091246