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Article

Molecular Study of Selected Taxonomically Critical Taxa of the Genus Iris L. from the Broader Alpine-Dinaric Area

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Institute of Agriculture and Tourism, Karla Huguesa 8, HR-52440 Poreč, Croatia
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Department of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
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Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
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Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb, Croatia
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Public Health Institute of the Istrian Region, Nazorova 23, HR-52100 Pula, Croatia
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Public Institution Natura Histrica, Riva 8, HR-52100 Pula, Croatia
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Medical and Chemical School, Ante Šupuk Street 29, HR-22000 Šibenik, Croatia
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Nature History Museum Split, Poljana kneza Trpimira 3, HR-21000 Split, Croatia
*
Author to whom correspondence should be addressed.
Joint last authors.
Plants 2020, 9(9), 1229; https://doi.org/10.3390/plants9091229
Received: 1 August 2020 / Revised: 10 September 2020 / Accepted: 16 September 2020 / Published: 18 September 2020
(This article belongs to the Section Plant Systematics, Taxonomy, Nomenclature and Classification)
Some wild, morphologically diverse taxa of the genus Iris in the broad Alpine-Dinaric area have never been explored molecularly, and/or have ambiguous systematic status. The main aims of our research were to perform a molecular study of critical Iris taxa from that area (especially a narrow endemic accepted species I. adriatica, for which we also analysed genome size) and to explore the contribution of eight microsatellites and highly variable chloroplast DNA (ndhJ, rpoC1) markers to the understanding of the Iris taxa taxonomy and phylogeny. Both the microsatellite-based UPGMA and plastid markers-based maximum likelihood analysis discriminated three main clusters in the set of 32 analysed samples, which correspond well to the lower taxonomic categories of the genus, and support separate status of ambiguous regional taxa (e.g., I. sibirica subsp. erirrhiza, I. x croatica and I. x rotschildii). The first molecular data on I. adriatica revealed its genome size (2C = 12.639 ± 0.202 pg) and indicated the existence of ecotypes. For future molecular characterisation of the genus we recommend the utilisation of microsatellite markers supplemented with a combination of plastid markers. View Full-Text
Keywords: Iridaceae; Europe; chloroplast DNA; microsatellites; phylogeny; taxonomy Iridaceae; Europe; chloroplast DNA; microsatellites; phylogeny; taxonomy
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MDPI and ACS Style

Weber, T.; Jakše, J.; Sladonja, B.; Hruševar, D.; Landeka, N.; Brana, S.; Bohanec, B.; Milović, M.; Vladović, D.; Mitić, B.; Poljuha, D. Molecular Study of Selected Taxonomically Critical Taxa of the Genus Iris L. from the Broader Alpine-Dinaric Area. Plants 2020, 9, 1229. https://doi.org/10.3390/plants9091229

AMA Style

Weber T, Jakše J, Sladonja B, Hruševar D, Landeka N, Brana S, Bohanec B, Milović M, Vladović D, Mitić B, Poljuha D. Molecular Study of Selected Taxonomically Critical Taxa of the Genus Iris L. from the Broader Alpine-Dinaric Area. Plants. 2020; 9(9):1229. https://doi.org/10.3390/plants9091229

Chicago/Turabian Style

Weber, Tim, Jernej Jakše, Barbara Sladonja, Dario Hruševar, Nediljko Landeka, Slavko Brana, Borut Bohanec, Milenko Milović, Dalibor Vladović, Božena Mitić, and Danijela Poljuha. 2020. "Molecular Study of Selected Taxonomically Critical Taxa of the Genus Iris L. from the Broader Alpine-Dinaric Area" Plants 9, no. 9: 1229. https://doi.org/10.3390/plants9091229

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