Next Article in Journal
Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid—Mediated Drought Response in Tomato
Next Article in Special Issue
The Genus Pratylenchus (Nematoda: Pratylenchidae) in Israel: From Taxonomy to Control Practices
Previous Article in Journal
Zinc-lysine Supplementation Mitigates Oxidative Stress in Rapeseed (Brassica napus L.) by Preventing Phytotoxicity of Chromium, When Irrigated with Tannery Wastewater
Previous Article in Special Issue
Ficus microcarpa Bonsai “Tiger bark” Parasitized by the Root-Knot Nematode Meloidogyne javanica and the Spiral Nematode Helicotylenchus dihystera, a New Plant Host Record for Both Species
Article

Bacterial Microbiota Isolated from Cysts of Globodera rostochiensis (Nematoda: Heteroderidae)

1
Department of Plant Diseases, Institute for Plant Protection and Environment, 11000 Belgrade, Serbia
2
Department of Agrochemistry, Institute of Soil Science, Department of Microbiology, 11000 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Plants 2020, 9(9), 1146; https://doi.org/10.3390/plants9091146
Received: 16 July 2020 / Revised: 28 August 2020 / Accepted: 1 September 2020 / Published: 4 September 2020
(This article belongs to the Special Issue Plant Parasitic Nematodes)
The potato cyst nematode (PCN) Globodera rostochiensis is a plant parasite of potato classified into a group of quarantine organisms causing high economic losses worldwide. Due to the long persistence of the parasite in soil, cysts harbor numerous bacteria whose presence can lead to cyst death and population decline. The cysts of G. rostochiensis found in two potato fields were used as a source of bacteria. The universal procedure was applied to extract DNA from bacteria which was then sequenced with 16S primers. The aims of the study were to identify bacterial microbiota associated with the PCN populations and to infer their phylogenetic relationships based on the maximum likelihood and Bayesian phylogeny of the 16S sequences. In addition, the impact of the most significant climate and edaphic factors on bacterial diversity were evaluated. Regarding the higher taxonomy, our results indicate that the prevalent bacterial classes were Bacilli, Actinobacteria and Alphaproteobacteria. Phylogenetic analyses clustered Brevibacterium frigoritolerans within the family Bacillaceae, confirming its recent reclassification. Long-term climate factors, such as air temperature, insolation hours, humidity and precipitation, as well as the content of soil organic matter, affected the bacterial diversity. The ability of cyst nematodes to persist in soil for a long time qualifies them as a significant natural source to explore the soil bacterial microbiota. View Full-Text
Keywords: potato cyst nematodes; Bacilli; Actinobacteria; Alphaproteobacteria; 16S; maximum likelihood; Bayesian inference; climate and edaphic factors potato cyst nematodes; Bacilli; Actinobacteria; Alphaproteobacteria; 16S; maximum likelihood; Bayesian inference; climate and edaphic factors
Show Figures

Figure 1

MDPI and ACS Style

Oro, V.; Knezevic, M.; Dinic, Z.; Delic, D. Bacterial Microbiota Isolated from Cysts of Globodera rostochiensis (Nematoda: Heteroderidae). Plants 2020, 9, 1146. https://doi.org/10.3390/plants9091146

AMA Style

Oro V, Knezevic M, Dinic Z, Delic D. Bacterial Microbiota Isolated from Cysts of Globodera rostochiensis (Nematoda: Heteroderidae). Plants. 2020; 9(9):1146. https://doi.org/10.3390/plants9091146

Chicago/Turabian Style

Oro, Violeta, Magdalena Knezevic, Zoran Dinic, and Dusica Delic. 2020. "Bacterial Microbiota Isolated from Cysts of Globodera rostochiensis (Nematoda: Heteroderidae)" Plants 9, no. 9: 1146. https://doi.org/10.3390/plants9091146

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop