Chemical Composition of a Supercritical Fluid (Sfe-CO2) Extract from Baeckea frutescens L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (Camellia sinensis (L.) O. Kuntze)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemical Composition of the B. Frutescens Leaf SFE-CO2 Extract
2.2. Contact Antifungal Activity of B. frutescens SFE-CO2 Extract
2.3. Screening for Fumigation Antifungal Activities of Major Chemical Compounds
2.4. Contact Antifungal Activity of Major Activity Components
3. Materials and Methods
3.1. Plant Materials
3.2. Chemicals
3.3. Isolation and Identification of Fungal Pathogens
3.4. Supercritical Fluid (SFE-CO2) Extract of Baeckea frutescens L.
3.5. Contact Antifungal Activities Bioassay
3.6. Fumigation Antifungal Activities Bioassay
3.7. B. frutescens Extract GC-MS Analysis
3.8. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Peak NO | Compound Name | RI (C) 1 | RI (L) 2 | Relative Area (%) 3 | Identification Methods |
---|---|---|---|---|---|
1 | α-Pinene | 940 | 944 | 2.11 | RI, MS, STD 4 |
2 | β-Pinene | 987 | 979 | 5.21 | RI, MS, STD |
3 | o- Cymene | 1044 | 1042 | 1.18 | RI, MS |
4 | Eucalyptol | 1049 | 1047 | 5.46 | RI, MS, STD |
5 | γ-Terpinene | 1074 | 1063 | 2.49 | RI, MS, STD |
6 | α-Terpinolene | 1104 | 1097 | 0.38 | RI, MS |
7 | Linalool | 1115 | 1104 | 1.47 | RI, MS, STD |
8 | Terpinen-4-ol | 1194 | 1182 | 0.83 | RI, MS, STD |
9 | Terpineol | 1209 | 1200 | 4.54 | RI, MS, STD |
10 | Epicubebol | 1433 | 1494 | 0.19 | RI, MS |
11 | β-Caryophyllene | 1448 | 1439 | 28.05 | RI, MS, STD |
12 | α-Caryophyllene | 1485 | 1452 | 24.02 | RI, MS, STD |
13 | α-Muurolene | 1520 | 1500 | 1.60 | RI, MS |
14 | δ-Guaiene | 1523 | 1502 | 0.57 | RI, MS |
15 | δ-Cadinene | 1536 | 1524 | 6.29 | RI, MS |
16 | trans-Calamenene | 1542 | 1527 | 1.28 | RI, MS |
17 | 6-epi-Shyobunol | 1548 | 1548 | 0.29 | RI, MS |
18 | Cadine-1,4-diene | 1552 | 1546 | 0.64 | RI, MS |
19 | Isoshyobunone | 1605 | 1562 | 1.63 | RI, MS |
20 | Caryophyllene oxide | 1610 | 1581 | 0.70 | RI, MS |
21 | Unkown | 1618 | - | 0.77 | RI, MS |
22 | Humulene epoxide II | 1624 | 1616 | 0.86 | RI, MS |
23 | Unkown | 1633 | - | 0.38 | RI, MS |
24 | α-Acorenol | 1643 | 1630 | 0.73 | RI, MS |
25 | Cubenol | 1648 | 1637 | 2.81 | RI, MS |
26 | Longifolenaldehyde | 1656 | 1631 | 2.40 | RI, MS |
27 | β-Acorenol | 1661 | 1649 | 2.11 | RI, MS |
Total | 98.99 |
Content (μg/mL) | Inhibitory Rate (%) 1 | |
---|---|---|
P. camelliae | C. gloeosporioides | |
50 | 66.63 ± 2.05 a,2 | 64.59 ± 1.59 a |
25 | 65.77 ± 1.35 a | 63.94 ± 0.39 a |
12.5 | 63.09 ± 2.84 a | 61.69 ± 0.46 a |
6 | 54.11 ± 1.18 b | 53.05 ± 0.87 b |
3 | 32.48 ± 4.37 c | 40.29 ± 1.65 c |
Control | 0.00 ± 1.08 d | 0.00 ± 0.16 d |
MIC50 | 5.11 μg/mL | 4.79 μg/mL |
Compound | P. camelliae | C. gloeosporioides | ||||||
---|---|---|---|---|---|---|---|---|
MIC50 (μL/mL) | 95% CI 1 (μL/mL) | Chi Square (χ2) | R2 | MIC50 (μL/mL) | 95% CI (μL/mL) | Chi Square (χ2) | R2 | |
Terpineol | 0.69 | 0.66–0.72 | 1.40 | 0.999 | 0.62 | 0.49–0.75 | 9.88 | 0.988 |
Linalool | 0.73 | 0.63–0.82 | 10.61 | 0.994 | 0.93 | 0.74–1.13 | 12.32 | 0.990 |
Terpinen-4-ol | 0.86 | 0.69–1.09 | 25.82 | 0.986 | 0.94 | 0.66–1.21 | 23.90 | 0.987 |
Eucalyptol | 2.79 | 2.62–2.97 | 6.50 | 0.980 | 2.18 | 2.05–2.3 | 5.07 | 0.981 |
Carbendazim | 9.70 | 9.41–10.01 | 0.55 | 0.999 | 0.095 | 0.091–0.100 | 1.22 | 0.999 |
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Jiang, H.; Zhang, M.; Qin, L.; Wang, D.; Yu, F.; Liang, W.; Song, C.; Granato, D. Chemical Composition of a Supercritical Fluid (Sfe-CO2) Extract from Baeckea frutescens L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (Camellia sinensis (L.) O. Kuntze). Plants 2020, 9, 1119. https://doi.org/10.3390/plants9091119
Jiang H, Zhang M, Qin L, Wang D, Yu F, Liang W, Song C, Granato D. Chemical Composition of a Supercritical Fluid (Sfe-CO2) Extract from Baeckea frutescens L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (Camellia sinensis (L.) O. Kuntze). Plants. 2020; 9(9):1119. https://doi.org/10.3390/plants9091119
Chicago/Turabian StyleJiang, Hao, Mengting Zhang, Li Qin, Dongxu Wang, Feng Yu, Wenhui Liang, Chuankui Song, and Daniel Granato. 2020. "Chemical Composition of a Supercritical Fluid (Sfe-CO2) Extract from Baeckea frutescens L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (Camellia sinensis (L.) O. Kuntze)" Plants 9, no. 9: 1119. https://doi.org/10.3390/plants9091119