Insights into the Role of Streptomyces hydrogenans as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against Meloidogyne incognita in Solanum lycopersicum Seedlings
Abstract
:1. Introduction
2. Results
2.1. Morphological Parameters
2.2. Effect of Biometabolites Produced by S. hydrogenans Strain DH-16 on the Photosynthetic Pigment of S. lycopersicum Seedlings 7 DAI
2.3. Malondialdehyde (MDA) and H2O2 Content
2.4. Antioxidative Enzyme Activities
2.5. Non-Enzymatic Antioxidants
2.6. Phenolic Compounds
2.7. Confocal Microscopy
3. Discussion
4. Materials and Methods
4.1. Production of Secondary Metabolites
4.2. Plant Material and Treatments
4.3. Morphological Parameters
4.4. Determination of Carotenoid and Chlorophyll Contents
4.5. Determination of Malondialdehyde (MDA) Content and H2O2 Content
4.6. Protein Content and Antioxidative Enzymes
4.7. Non Enzymatic Antioxidants
4.8. Estimation of Phenolic Compounds
4.9. Confocal Microscopy
4.10. Statictical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Treatments | Parameters (Mean ± S.E.) | |||||
---|---|---|---|---|---|---|
Germination % | Root Length (cm) | Shoot Length (cm) | Root Weight (g) | Shoot Weight (g) | No. of Galls | |
C | 70.67 ± 1.453 a | 8.827 ± 0.361 bcd | 7.337 ± 0.130 a | 0.150 ± 0.012 ab | 0.5600 ± 0.021 a | - |
NI | 69.33 ± 1.202 a | 6.897 ± 0.529 a | 6.943 ± 0.135 a | 0.127 ± 0.008 a | 0.533 ± 0.015 a | 8 ± 0.333 a |
S | 87 ± 1.527 b | 9.130 ± 0.460 cd | 7.9167 ± 0.202 ab | 0.170 ± 0.006 b | 0.693 ± 0.009 b | - |
S + NI | 85.67 ± 1.202 b | 7.173 ± 0.061 ab | 8.620 ± 0.399 b | 0.1633 ± 0.003 ab | 0.690 ± 0.023 b | 6 ± 1.202 a |
E | 86.67 ± 1.333 b | 9.150 ± 0.105 d | 7.456 ± 0.059 a | 0.170 ± 0.100 b | 0.690 ± 0.006 b | - |
E + NI | 84 ± 1.732 b | 7.287 ± 0.525 abc | 7.157 ± 0.206 a | 0.153 ± 0.008 ab | 0.580 ± 0.012 a | 7 ± 0.575 a |
F-value | 33.747 ** | 7.440 ** | 7.903 ** | 3.733 * | 23.431 ** | 52.235 (ns) |
Treatments | Parameters (Mean ± S.E.) | |||||
---|---|---|---|---|---|---|
Catalase (CAT) (U/mg Protein) | Superoxide Dismutase (SOD) (U/mg Protein) | Ascorbate Peroxidise (APOX) (U/mg Protein) | Polyphenol Oxidise (PPO) (U/mg Protein) | Guaiacol Peroxidise (GuPOX) (U/mg Protein) | Glutathione-S-Transferase (GST) (U/mg Protein) | |
C | 0.1249 ± 0.0195 a | 0.0030 ± 0.00028 a | 0.0651 ± 0.00451 a | 0.0119 ± 0.00040 a | 0.0534 ± 0.00266 a | 0.0471 ± 0.00375 a |
NI | 0.1353 ± 0.0175 a | 0.0039 ± 0.00028 abc | 0.0886 ± 0.00426 b | 0.0138 ± 0.00048 a | 0.0827 ± 0.00632 b | 0.0659 ± 0.01176 ab |
S | 0.1487 ± 0.0106 a | 0.0043 ± 0.00017 bc | 0.0809 ± 0.00330 ab | 0.0130 ± 0.00024 a | 0.0715 ± 0.00576 ab | 0.0591 ± 0.00452 ab |
S + NI | 0.1407 ± 0.0133 a | 0.0045 ± 0.00032 c | 0.0918 ± 0.00070 b | 0.0140 ± 0.00033 a | 0.0954 ± 0.00796 b | 0.0791 ± 0.00279 b |
E | 0.1357 ± 0.0042 a | 0.0032 ± 0.00010 ab | 0.0777 ± 0.00509 ab | 0.0128 ± 0.00083 a | 0.699 ± 0.00829 ab | 0.0503 ± 0.00321 ab |
E + NI | 0.138 ± 0.00493 a | 0.0037 ± 0.00015 abc | 0.0908 ± 0.00474 b | 0.0139 ± 0.00016 a | 0.0950 ± 0.00111 b | 0.0753 ± 0.00890 ab |
F-value | 0.355 (ns) | 6.632 ** | 6.372 ** | 3.174 (ns) | 7.452 ** | 3.761 * |
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Sharma, N.; Khanna, K.; Manhas, R.K.; Bhardwaj, R.; Ohri, P.; Alkahtani, J.; Alwahibi, M.S.; Ahmad, P. Insights into the Role of Streptomyces hydrogenans as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against Meloidogyne incognita in Solanum lycopersicum Seedlings. Plants 2020, 9, 1109. https://doi.org/10.3390/plants9091109
Sharma N, Khanna K, Manhas RK, Bhardwaj R, Ohri P, Alkahtani J, Alwahibi MS, Ahmad P. Insights into the Role of Streptomyces hydrogenans as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against Meloidogyne incognita in Solanum lycopersicum Seedlings. Plants. 2020; 9(9):1109. https://doi.org/10.3390/plants9091109
Chicago/Turabian StyleSharma, Nandni, Kanika Khanna, Rajesh Kumari Manhas, Renu Bhardwaj, Puja Ohri, Jawaher Alkahtani, Mona S. Alwahibi, and Parvaiz Ahmad. 2020. "Insights into the Role of Streptomyces hydrogenans as the Plant Growth Promoter, Photosynthetic Pigment Enhancer and Biocontrol Agent against Meloidogyne incognita in Solanum lycopersicum Seedlings" Plants 9, no. 9: 1109. https://doi.org/10.3390/plants9091109