Light and Low Relative Humidity Increase Antioxidants Content in Mung Bean (Vigna radiata L.) Sprouts
Abstract
:1. Introduction
2. Results
2.1. Sprouts Development
2.2. Morpho-Anatomical Analysis of Hypocotyls
2.3. Content of Anthocyanins and Polyphenols, and FRAP Assay
2.4. Soluble Sugar and Starch Quantification
3. Discussion
4. Materials and Methods
4.1. Experimental Design and Plant Material
4.2. Morpho-Anatomical Analyses
4.3. Polyphenol and Anthocyanin Content
4.4. FRAP Assay
4.5. Soluble Sugar and Starch Quantification
4.6. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Treatments | Hypocotyl Length (cm) | Root Length (cm) | Total Length (cm) | Fresh Weight (g) | Dry Weight (g) |
---|---|---|---|---|---|
Relative humidity (RH) | |||||
Low | 2.53 b | 0.88 b | 3.74 b | 0.22 b | 0.044 a |
High | 3.47 a | 1.93 a | 3.95 a | 0.46 a | 0.044 a |
Light regime (LR) | |||||
Light | 2.69 b | 1.18 b | 3.55 b | 0.32 b | 0.048 a |
Dark | 3.32a | 1.62 a | 4.13 a | 0.37 a | 0.046 a |
Interaction | |||||
LowRH × light | 2.26 a | 0.63 a | 3.40 a | 0.20 a | 0.047 a |
HighRH × light | 3.12 a | 1.73 a | 3.71 a | 0.44 a | 0.049 a |
LowRH × dark | 2.80 a | 1.13 a | 4.08 a | 0.25 a | 0.042 a |
HighRH × dark | 3.83 a | 2.12 a | 4.19 a | 0.49 a | 0.042 a |
Significance | |||||
RH | *** | *** | *** | *** | NS |
LR | *** | *** | *** | * | NS |
RH × LR | NS | NS | NS | NS | NS |
Treatments | TCC (µm) | SD (µm) | TD (µm) | CL-CC (n) | CL-S (n) | NC-CC (n mm−2) | NC-S (n mm−2) | NPB (n mm−2) | DPB (µm) |
---|---|---|---|---|---|---|---|---|---|
Relative humidity (RH) | |||||||||
Low | 963.75 a | 1418.13 b | 2381.88 a | 10.05 a | 16.37 a | 25.35 a | 21.10 a | 40.07 a | 4.0 a |
High | 817.14 b | 1554.28 a | 2371.43 a | 10.02 a | 17.82 a | 23.77 a | 18.02 b | 27.54 b | 3.75 a |
Light regime (LR) | |||||||||
Light | 828.46 b | 1429.33 b | 2257.80 b | 9.8 b | 17.45 a | 29.67 a | 23.10 a | 40.76 a | 4.67 a |
Dark | 952.43 a | 1543.07 a | 2495.51 a | 10.27 a | 16.75 a | 19.45 b | 16.02 b | 26.85 b | 3.07 b |
Interaction (RH × LR) | |||||||||
LowRH × light | 886.56 b | 1322.80 b | 2209.36 b | 9.50 b | 16.75 a | 35.45 a | 25.50 a | 45.43 a | 5.1 a |
HighRH × light | 770.38 c | 1535.87 a | 2306.25 b | 10.10 ab | 18.15 a | 23.90 b | 20.70 b | 36.09 b | 4.25 b |
LowRH × dark | 1040.94 a | 1513.46 a | 2554.40 a | 10.60 a | 16.50 a | 15.25 c | 16.70 c | 34.71 b | 2.9 c |
HighRH × dark | 863.92 bc | 1572.69 a | 2436.62 a | 10.06 ab | 17.50 a | 23.65 b | 15.35 c | 18.99 c | 3.25 c |
Significance | |||||||||
RH | *** | *** | NS | NS | NS | NS | *** | *** | NS |
LR | ** | *** | *** | * | NS | *** | *** | *** | *** |
RH × LR | * | ** | * | * | NS | *** | * | * | ** |
Treatments | Anthocyanins (mg g−1) | Polyphenols (mg GAE mg−1) | Antioxidant Activity (µmol TE/mg−1) | Soluble Sugars (µmol (glucose) Eq. g−1) | Starch (µmol (glucose) Eq. g−1) |
---|---|---|---|---|---|
Relative humidity (RH) | |||||
Low | 0.85 a | 0.022 a | 1.01 a | 0.24 b | 0.047 a |
High | 0.68 b | 0.013 b | 0.57 b | 0.53 a | 0.027 b |
Light regime (LR) | |||||
Light | 0.93 a | 0.026 a | 1.02 a | 0.33 b | 0.046 a |
Dark | 0.60 b | 0.011 b | 0.55 b | 0.44 a | 0.028 b |
Significance | |||||
RH | *** | *** | *** | *** | * |
LR | *** | *** | *** | ** | * |
RH × LR | * | * | *** | ** | * |
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Amitrano, C.; Arena, C.; De Pascale, S.; De Micco, V. Light and Low Relative Humidity Increase Antioxidants Content in Mung Bean (Vigna radiata L.) Sprouts. Plants 2020, 9, 1093. https://doi.org/10.3390/plants9091093
Amitrano C, Arena C, De Pascale S, De Micco V. Light and Low Relative Humidity Increase Antioxidants Content in Mung Bean (Vigna radiata L.) Sprouts. Plants. 2020; 9(9):1093. https://doi.org/10.3390/plants9091093
Chicago/Turabian StyleAmitrano, Chiara, Carmen Arena, Stefania De Pascale, and Veronica De Micco. 2020. "Light and Low Relative Humidity Increase Antioxidants Content in Mung Bean (Vigna radiata L.) Sprouts" Plants 9, no. 9: 1093. https://doi.org/10.3390/plants9091093