An AC-Rich Bean Element Serves as an Ethylene-Responsive Element in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. ACE Causes the Minimal Promoter to Respond to Ethylene in Arabidopsis Seedlings
2.2. ACE Specifically Binds to the Nuclear Proteins of Arabidopsis
2.3. The First 9-bp Fragment of ACE Is Required by Nuclear Protein Binding
2.4. PvMTF-1 and PvERF15 Respond to Ethylene Stress in Bean Seedlings
3. Discussion
3.1. Our Findings Provide Evidence for ACE as a Novel ERE in Arabidopsis
3.2. The Biological Implication of ACE in the Ethylene Induction of PvMTF-1
4. Materials and Methods
4.1. Plant Materials and Growing Conditions
4.2. Agrobacterium Tumefaciens-Mediated Gene Transfer by Infiltration of Arabidopsis Seedlings
4.3. Protein Extraction
4.4. EMSA
4.5. Western Blot Analysis
4.6. RNA Isolation and cDNA Synthesis
4.7. Real-Time Quantitative Reverse Transcription (qRT)-PCR Analysis
4.8. Bioinformatics Analysis
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, C.; Lin, T.; Wang, M.; Qi, X. An AC-Rich Bean Element Serves as an Ethylene-Responsive Element in Arabidopsis. Plants 2020, 9, 1033. https://doi.org/10.3390/plants9081033
Wang C, Lin T, Wang M, Qi X. An AC-Rich Bean Element Serves as an Ethylene-Responsive Element in Arabidopsis. Plants. 2020; 9(8):1033. https://doi.org/10.3390/plants9081033
Chicago/Turabian StyleWang, Chunying, Tingting Lin, Mengqi Wang, and Xiaoting Qi. 2020. "An AC-Rich Bean Element Serves as an Ethylene-Responsive Element in Arabidopsis" Plants 9, no. 8: 1033. https://doi.org/10.3390/plants9081033