Next Article in Journal
Tumorous Stem Development of Brassica Juncea: A Complex Regulatory Network of Stem Formation and Identification of Key Genes in Glucosinolate Biosynthesis
Previous Article in Journal
The Selection of Gamma-Ray Irradiated Higher Yield Rice Mutants by Directed Evolution Method
Previous Article in Special Issue
Comparison of Biostimulant Treatments in Acmella oleracea Cultivation for Alkylamides Production
Open AccessArticle

Substrate and Plant Genotype Strongly Influence the Growth and Gene Expression Response to Trichoderma afroharzianum T22 in Sugar Beet

1
Department of Biology, Lund University, Sölvegatan 35B, SE–223 62 Lund, Sweden
2
MariboHilleshög AB, Säbyholmsv. 24, 261 91 Landskrona, Sweden
3
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Box 102, SE–23053 Alnarp, Sweden
*
Author to whom correspondence should be addressed.
Plants 2020, 9(8), 1005; https://doi.org/10.3390/plants9081005
Received: 14 July 2020 / Revised: 29 July 2020 / Accepted: 3 August 2020 / Published: 7 August 2020
(This article belongs to the Special Issue Biostimulants in Plants Science)
Many strains of Trichoderma fungi have beneficial effects on plant growth and pathogen control, but little is known about the importance of plant genotype, nor the underlying mechanisms. We aimed to determine the effect of sugar beet genotypic variation on Trichoderma biostimulation. The effect of Trichoderma afroharzianum T22 on sugar beet inbred genotypes were investigated in soil and on sterile agar medium regarding plant growth, and by quantitative reverse transcriptase-linked polymerase chain reaction (qRT-PCR) analysis for gene expression. In soil, T22 application induced up to 30% increase or decrease in biomass, depending on plant genotype. In contrast, T22 treatment of sterile-grown seedlings resulted in a general decrease in fresh weight and root length across all sugar beet genotypes. Root colonization of T22 did not vary between the sugar beet genotypes. Sand- and sterile-grown roots were investigated by qRT-PCR for expression of marker genes for pathogen response pathways. Genotype-dependent effects of T22 on, especially, the jasmonic acid/ethylene expression marker PR3 were observed, and the effects were further dependent on the growth system used. Thus, both growth substrate and sugar beet genotype strongly affect the outcome of inoculation with T. afroharzianum T22. View Full-Text
Keywords: Beta vulgaris; gene expression; growth stimulation; inbred genotypes; pathogen response pathways; Trichoderma afroharzianum Beta vulgaris; gene expression; growth stimulation; inbred genotypes; pathogen response pathways; Trichoderma afroharzianum
Show Figures

Graphical abstract

MDPI and ACS Style

Schmidt, J.; Dotson, B.R.; Schmiderer, L.; van Tour, A.; Kumar, B.; Marttila, S.; Fredlund, K.M.; Widell, S.; Rasmusson, A.G. Substrate and Plant Genotype Strongly Influence the Growth and Gene Expression Response to Trichoderma afroharzianum T22 in Sugar Beet. Plants 2020, 9, 1005.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop