Heterologous Expression of the Constitutive Disease Resistance 2 and 8 Genes from Poncirus trifoliata Restored the Hypersensitive Response and Resistance of Arabidopsis cdr1 Mutant to Bacterial Pathogen Pseudomonas syringae
Abstract
:1. Introduction
2. Results
2.1. Cloning and Structure of Constitutive Disease Resistance 2 and 8 from Poncirus trifoliata
2.2. PtCDR2 and PtCDR8 Restored the Hypersensitive Response of Arabidopsis cdr1 Mutant to Pathogen
2.3. PtCDR2 and PtCDR8 Inhibited Pst DC3000 avrRpt2 Growth
2.4. PtCDR2 and PtCDR8 Increased the Expression Level of PATHOGENESIS RELATED 1 (PR1) in the cdr1 Mutant
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Sequence Analysis
4.3. Gene Cloning, Construction of Expression Vectors, and Transformation of Arabidopsis cdr1 Mutant
4.4. Disease Assay
4.5. Gene Expression Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ying, X.; Redfern, B.; Gmitter, F.G., Jr.; Deng, Z. Heterologous Expression of the Constitutive Disease Resistance 2 and 8 Genes from Poncirus trifoliata Restored the Hypersensitive Response and Resistance of Arabidopsis cdr1 Mutant to Bacterial Pathogen Pseudomonas syringae. Plants 2020, 9, 821. https://doi.org/10.3390/plants9070821
Ying X, Redfern B, Gmitter FG Jr., Deng Z. Heterologous Expression of the Constitutive Disease Resistance 2 and 8 Genes from Poncirus trifoliata Restored the Hypersensitive Response and Resistance of Arabidopsis cdr1 Mutant to Bacterial Pathogen Pseudomonas syringae. Plants. 2020; 9(7):821. https://doi.org/10.3390/plants9070821
Chicago/Turabian StyleYing, Xiaobao, Bryce Redfern, Frederick G. Gmitter, Jr., and Zhanao Deng. 2020. "Heterologous Expression of the Constitutive Disease Resistance 2 and 8 Genes from Poncirus trifoliata Restored the Hypersensitive Response and Resistance of Arabidopsis cdr1 Mutant to Bacterial Pathogen Pseudomonas syringae" Plants 9, no. 7: 821. https://doi.org/10.3390/plants9070821