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Open AccessArticle

High-Throughput Sequencing Facilitates Discovery of New Plant Viruses in Poland

1
Department of Virology and Bacteriology, Institute of Plant Protection–National Research Institute, Wegorka 20, 60-318 Poznan, Poland
2
Plant Disease Clinic and Bank of Pathogens, Institute of Plant Protection–National Research Institute, Wegorka 20, 60-318 Poznan, Poland
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(7), 820; https://doi.org/10.3390/plants9070820
Received: 1 May 2020 / Revised: 22 June 2020 / Accepted: 26 June 2020 / Published: 29 June 2020
(This article belongs to the Special Issue Diagnosis and Control of Plant Viral Diseases)
Viruses cause epidemics on all major crops of agronomic importance, and a timely and accurate identification is essential for control. High throughput sequencing (HTS) is a technology that allows the identification of all viruses without prior knowledge on the targeted pathogens. In this paper, we used HTS technique for the detection and identification of different viral species occurring in single and mixed infections in plants in Poland. We analysed various host plants representing different families. Within the 20 tested samples, we identified a total of 13 different virus species, including those whose presence has not been reported in Poland before: clover yellow mosaic virus (ClYMV) and melandrium yellow fleck virus (MYFV). Due to this new finding, the obtained sequences were compared with others retrieved from GenBank. In addition, cucurbit aphid-borne yellows virus (CABYV) was also detected, and due to the recent occurrence of this virus in Poland, a phylogenetic analysis of these new isolates was performed. The analysis revealed that CABYV population is highly diverse and the Polish isolates of CABYV belong to two different phylogenetic groups. Our results showed that HTS-based technology is a valuable diagnostic tool for the identification of different virus species originating from variable hosts, and can provide rapid information about the spectrum of plant viruses previously not detected in a region. View Full-Text
Keywords: virus; virus identification; high-throughput sequencing; phylogenetic analysis virus; virus identification; high-throughput sequencing; phylogenetic analysis
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Minicka, J.; Zarzyńska-Nowak, A.; Budzyńska, D.; Borodynko-Filas, N.; Hasiów-Jaroszewska, B. High-Throughput Sequencing Facilitates Discovery of New Plant Viruses in Poland. Plants 2020, 9, 820.

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