Bacterial Compound N,N-Dimethylhexadecylamine Modulates Expression of Iron Deficiency and Defense Response Genes in Medicago truncatula Independently of the Jasmonic Acid Pathway
Abstract
:1. Introduction
2. Results
2.1. Effect of Iron Deficiency on M. truncatula Growth
2.2. Effect of SA, JA, and DMHDA on M. truncatula Growth
2.3. Effect of Iron Deficiency on the Expression of Iron-Deficiency and Biotic Stress-Response Genes
2.4. Effect of SA and JA on the Expression of Biotic Stress and Iron-Deficiency Response Genes
2.5. Effect of DMHDA on the Expression of Biotic Stress and Iron-Deficiency Response Genes
2.6. Effect of P. syringae and B. cinerea the Expression of Biotic Stress and Iron-Deficiency Response Genes
2.7. Effect of DMHDA on Growth of M. truncatula Plants Infected with P. syringae or B. cinerea
3. Discussion
4. Materials and Methods
4.1. M. truncatula Seed Germination
4.2. MS Medium Preparation
4.3. Chemicals
4.4. Growth and Gene Expression Evaluation
4.5. P. syringae and B. cinerea Inoculation
4.6. Growth Analysis and Quantification of Chlorophyll Content in M. truncatula
4.7. Identification of Defense and Iron-Deficiency Genes
4.8. RNA Extraction and cDNA Synthesis
4.9. RT-qPCR
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Montejano-Ramírez, V.; García-Pineda, E.; Valencia-Cantero, E. Bacterial Compound N,N-Dimethylhexadecylamine Modulates Expression of Iron Deficiency and Defense Response Genes in Medicago truncatula Independently of the Jasmonic Acid Pathway. Plants 2020, 9, 624. https://doi.org/10.3390/plants9050624
Montejano-Ramírez V, García-Pineda E, Valencia-Cantero E. Bacterial Compound N,N-Dimethylhexadecylamine Modulates Expression of Iron Deficiency and Defense Response Genes in Medicago truncatula Independently of the Jasmonic Acid Pathway. Plants. 2020; 9(5):624. https://doi.org/10.3390/plants9050624
Chicago/Turabian StyleMontejano-Ramírez, Vicente, Ernesto García-Pineda, and Eduardo Valencia-Cantero. 2020. "Bacterial Compound N,N-Dimethylhexadecylamine Modulates Expression of Iron Deficiency and Defense Response Genes in Medicago truncatula Independently of the Jasmonic Acid Pathway" Plants 9, no. 5: 624. https://doi.org/10.3390/plants9050624