Next Article in Journal
Functional Traits, Morphology, and Herbage Production of Vernalised and Non-Vernalised Chicory cv. Choice (Cichorium intybus L.) in Response to Defoliation Frequency and Height
Next Article in Special Issue
The Use of Essential Oils from Thyme, Sage and Peppermint against Colletotrichum acutatum
Previous Article in Journal
Strigolactones Control Root System Architecture and Tip Anatomy in Solanum lycopersicum L. Plants under P Starvation
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:

The Extracts of Cinnamon and Clove as Potential Biofungicides against Strawberry Grey Mould

Laboratory of Plant Protection, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, LT-54333 Babtai, Kaunas district, Lithuania
Author to whom correspondence should be addressed.
Plants 2020, 9(5), 613;
Original submission received: 15 April 2020 / Revised: 7 May 2020 / Accepted: 7 May 2020 / Published: 11 May 2020


Biofungicides from plants are a possibility for the biocontrol of fungal diseases, as chemical products may be harmful to the environment and humans. Strawberry is one of the many plants infected by grey mould (Botrytis cinerea), and innovative methods of biocontrol against B. cinerea are under investigation. Clove (Syzygium aromaticum L.) and cinnamon (Cinnamomum cassia L.) accumulate natural compounds, such as eugenol and cinnamaldehyde, which provide antimicrobial and antifungal properties; thus, extracts of these plants could be possibly used as biofungicides. During this study, the inhibition of B. cinerea by clove and cinnamon extracts was evaluated in vitro on Petri plates and detached strawberry leaves; additionally, the chemical composition of volatiles was identified. Clove extract consisted of 52.88% eugenol, and cinnamon consisted of 74.67% cinnamaldehyde. The efficacy of the extracts on detached strawberry leaves showed that 12 mL L−1 concentration of clove extract was effective in suppressing the grey mould infection. Clove and cinnamon extracts showed an equal ability to inhibit B. cinerea on Petri plates. However, the results of the detached strawberry leaves assay showed that clove extract was more effective as a biocontrol product. Overall, clove extract expressed a high potential for application in biofungicides formulations.

Graphical Abstract

1. Introduction

Grey mould caused by Botrytis cinerea has a significant economic impact on plant production for a variety of different crops [1,2,3,4,5]. Strawberry is one of the hosts and can be damaged during different growth stages. Plant protection measures against fungal pathogens are necessary in farms in order to avoid yield and crop loss. However, chemical fungicides leave residues in the environment and in agricultural products and indirectly cause harm to humans. Following the guidance of integrated pest management, innovative ways to control pathogens should be found, with the aim of making agriculture more sustainable. Grey mould is conventionally controlled by chemical fungicides [6], and biocontrol products for strawberry are in demand [7].
Cinnamon (Cinnamomum cassia L.) extracts and essential oils have been investigated for their various medicinal properties [8,9,10,11,12]. For example, the cinnamon extract was examined for antifungal effects in bandages against human-pathogenic fungi [13]. Clove (Syzygium aromaticum L.) has been used in traditional medicine [14]. There are many reports on its antimicrobial and broad antifungal activities in the literature [2,15,16,17,18,19,20].
Plants accumulate secondary aromatic metabolites that have antimicrobial or antifungal activities [21]. As naturally occurring compounds may be an option for controlling fungal diseases [6,22,23], attention should be paid to the composition of natural compounds from plants and their extraction possibilities. The main constituent of clove essential oil is eugenol [24,25,26,27,28]. There are studies reporting the effectiveness of eugenol and other related compounds against B. cinerea [29,30]. The main components of cinnamon essential oil are cinnamaldehyde and trans-cinnamaldehyde [27]. Also, different percentages of eugenol can be found depending on the plant parts used for extraction [9,31]. Reports on the efficacy of clove and cinnamon oils against plant pathogens can be found in the literature [27,32,33,34,35]. It was previously found that both oils were effective at suppressing Rhizoctonia solani and Fusarium oxysporum, and eugenol and cinnamaldehyde are the derivatives, which showed antifungal activity against these pathogens [33]. Cinnamon oil was determined to have moderate antifungal activity against F. oxysporum f. sp. fragariae [32]. Additionally, a potential application of clove oil in nanoemulsions against F. oxysporum f. sp. lycopersici was investigated [36]. Cinnamon and clove extracts have the potential to be applied as biofungicides in the future due to their active components, which might be responsible for antifungal activities. As the main components of these two extracts belong to essential oils, the way to extract them should be considered. Supercritical CO2 extraction is an advanced and environmentally friendly method to obtain lipophilic compounds from plant material [37]. The possibility to apply clove oil obtained by supercritical CO2 extraction in agriculture as a phytotoxic agent was evaluated [26]. Meanwhile, in our study, subcritical CO2 extraction was chosen for producing plant extracts. Low process temperature and pressure allow extraction cost to be minimized and active biological compounds to be maintained, thus providing a higher possible fungicidal effect.
The application of the plant extracts as biocontrols on strawberry plants could be a preventative measure to avoid the contamination of fruits, soil and water with chemical residues, as the majority of the extracts are biodegradable and non-toxic. Various alternative ways for the control of Botrytis cinerea and other fungal pathogens in greenhouse and field conditions have been investigated [3,38,39,40]. The effectiveness of microbial biocontrol agents and seaweed extract was previously studied on strawberry leaves [41,42,43]. However, in reviewing the available literature, it was observed that there is a lack of studies on the antifungal activity of plant extracts against B. cinerea on detached strawberry leaves.
The antifungal activity of cinnamon and clove extracts against B. cinerea, especially on detached strawberry leaves, has not been widely investigated. By combining active ingredients from nature with environmentally friendly methods of extraction, new measures for sustainable agriculture could be created. This study aimed to evaluate the antifungal activity of cinnamon (Cinnamomum cassia L.) and clove (Syzygium aromaticum L.) extracts against strawberry grey mould (B. cinerea) in vitro and on detached strawberry leaves and also aimed to identify the chemical composition of volatile compounds.

2. Results

The chemical composition of the volatile compounds of CO2 extracts of C. cassia and S. aromaticum is presented in Table 1. In total, 98.86% of the compounds of cinnamon extract were identified and trans-cinnamaldehyde was determined to be the dominant compound. Meanwhile, based on 99.81% of the components identified in clove extract, eugenol, eugenol acetate and trans-caryophyllene were observed in the highest quantities.
The antifungal activity of cinnamon and clove extracts was investigated on PDA (potato dextrose agar) under different concentrations. The inhibition of B. cinerea by cinnamon extract is presented in Figure 1. This extract did not demonstrate inhibition at 200 μL L−1 at 4 and 7 DAI (days after inoculation) and reached 20% at 400 μL L−1 at 4 DAI. However, the mycelial growth of the pathogen was fully inhibited from 600 μL L−1 to the full range of concentrations.
The inhibition of B. cinerea by clove extract is shown in Figure 2. Clove extract expressed stronger antifungal activity at 200 μL L−1 and 400 μL L−1 and inhibited the growth of B. cinerea up to 80% at 4 DAI and 60% at 7 DAI.
Both tested extracts entirely suppressed the mycelial growth at 600–2200 μL L−1. The determined MIC (minimal inhibitory concentration) of cinnamon and clove extracts was low and equal to 600 μL L−1.
The fungicidal activities of C. cassia and S. aromaticum extracts were investigated by measuring the radial colony growth of B. cinerea after reinoculation. Results are shown in Table 2. Cinnamon extract demonstrated higher fungicidal activity than clove extract. The MFC (minimal fungicidal concentration) of the cinnamon extract was 600 μL L−1, as no visible growth of B. cinerea was observed 48 h after inoculation, while the MFC of the clove extract was 1400 μL L−1.
The detached strawberry leaves assay revealed that the clove extract had a higher inhibitory effect of the infection of grey mould (Figure 3). Among all of the investigated treatments, only 12 mL L−1 concentration of clove extract significantly reduced the infection on strawberry leaves compared to control (Figure 4). In comparison, 6 mL L−1 of both tested extracts increased the spread of grey mould, while 12 mL L−1 concentration of the cinnamon extract exhibited a low antifungal effect on detached leaves.

3. Discussion

Environmental problems and possible threats to human health resulting from extensive long-term use of chemical fungicides require alternative solutions. Previous research has evaluated the chemical compounds and antifungal activity of essential oils and extracts [32,33,34,35,36,40]. In this research, extracts from cinnamon (Cinnamomum cassia L.) and clove (Syzygium aromaticum L.) showed high antifungal activity against Botrytis cinerea in vitro.
It was determined that trans-cinnamaldehyde was the main component in the cinnamon extract, which supports the expected composition of extracts from cinnamon bark [9,35]. Eugenol was a significant compound in the clove bud extract. However, the determined amount of this compound (52.88%) in the volatile fraction of our extract was lower compared to the composition of the essential oils in other studies [25,28]. The differences in the composition may have been a result of the differences in the extraction methods or raw materials; however, the obtained result is opposite to the findings of Yazdani et al. [24], who observed a higher content of eugenol in the clove oil, which was produced by supercritical CO2 extraction and then steam distillation. Our clove extract had up to a 10% lower content of eugenol and a slightly higher amount of eugenol acetate compared to the studies of clove essential oils obtained under different supercritical CO2 extraction conditions [26].
The unique composition of the extracts provides a possibility for this material to be applied as biopesticides. Other studies [33] confirmed the high antifungal activity of the main constituents of cinnamon essential oil—cinnamaldehyde and clove essential oil—eugenol in vitro. Cinnamon bark oil and cinnamon oil showed strong fumigation and contact activities against the causal agent of false rice smut (Villosiclava virens) [35]. Moreover, the main determined component of both oils was trans-cinnamaldehyde, which decreased the sporulation of V. virens. In our study, trans-cinnamaldehyde-rich cinnamon extract showed high antifungal activity against B. cinerea. Wang et al. [29] determined that eugenol inhibited the mycelium growth of only B. cinerea and Sclerotinia sclerotiorum from ten different fungal plant pathogens. High sensitivity to eugenol containing clove extract could be seen in the results of our study, where high inhibition of B. cinerea was observed at the lowest investigated concentrations.
Our results contribute to the findings of Xie et al. [33], whose study showed that clove oil had higher antifungal activity than cinnamon oil against fungal pathogens Rhizoctonia solani and Fusarium oxysporum. Observed antifungal indexes of cinnamon oil were respectively 54.8% and 28.5% at a 400 μg mL−1, while clove oil reached 100.0% and 83.7% at 300 μg mL−1. In our study, a broader range of concentrations was investigated, and 100.0% inhibition of B. cinerea was achieved at 600 μL mL−1. In order to achieve complete inhibition, a higher concentration might be necessary due to the lower concentration of essential oil antifungal components in the whole extract compared to the pure essential oil in Xie et al.’s study [33]. Contrary to our results, Moghadam et al. [27] found cinnamon essential oil (95% cinnamaldehyde) to be more effective against mycotoxin producer Aspergillus ochraceus than clove essential oil (93% of eugenol). However, both oils had the highest antifungal activity from all tested oils in the mentioned study. Despite the lower concentration of eugenol in clove extract compared to other studies, high antifungal activity against B. cinerea was achieved. The antifungal effect of plant-derived products may vary depending on the pathogen, though our study supports the contribution of the main components of the oils to their effectiveness.
The mechanism of the antifungal activity of cinnamon and clove extract was not identified in this study. Melgarejo-Flores et al. [31] stated that the effectiveness of cinnamon leaf oil (Cinnamomum zeylanicum) against B. cinerea depends on the ability of the main constituents (eugenol and cinnamaldehyde) to disrupt cell walls and membranes. In this study, cinnamon and clove extract showed low MFC, which describe the ability not only to suppress the growth of the B. cinerea temporarily but also to damage it irreversibly. Properties of eugenol from clove oil to break fungal structures are supported by the study of Olea et al. [30]. As the components of both extracts in our study are eugenol and cinnamaldehyde, we could expect the same manner of suppressing/damaging the pathogen. The results of our study agree with the fact that extracts and essential oils rich in eugenol and cinnamaldehyde have high antifungal activities.
The application of seaweed Ascophyllum nodosum extract on detached strawberry leaves against spore germination of Podosphaera aphanis, the causal agent of powdery mildew, can be found in the literature [43]. To the best of our knowledge, no studies of cinnamon and clove extract antifungal activity have been previously performed on detached strawberry leaves. In the present research, only 12 mL L−1 of clove extract managed to inhibit the infection of grey mould on detached strawberry leaves. Cinnamon extract demonstrated low antifungal effect at 12 mL L−1; as such, for future experiments, a higher concentration of this extract should be examined for higher effectiveness. For both investigated extracts, increased mycelial growth of B. cinerea was observed at 6 mL L−1. Environmental stress caused to the pathogen by the extracts may be responsible for this. In that case, the main biological processes of the fungi, including growth, were even more active. We recommend testing higher concentrations of the extracts together with phytotoxicity in the future.
To conclude, clove extract showed the potential to suppress the infection of grey mould on detached strawberry leaves and has the potential to become a biofungicide for the biocontrol of pathogens affecting strawberry. Future research should aim to elucidate optimal concentrations for in situ applications.

4. Materials and Methods

4.1. The Extraction of Plant Material

The extracts of cinnamon bark (Cinnamomum cassia L.) and clove bud (Syzygium aromaticum L.) were chosen for the determination of antifungal activity against strawberry pathogen Botrytis cinerea at different concentrations. Dried cinnamon bark and clove bud were obtained from Saldva (Lithuania). Subcritical CO2 extraction was performed while producing cinnamon and clove extracts as described in our previous study [44]. Both extracts were kept at 4 °C until the experiments.

4.2. Identification of the Extracts Chemical Composition

Volatile compounds of extracts were determined by gas chromatography/mass spectrometry (GC-MS). The analysis was performed on GC-2010Plus/GCMS-QP2010 Ultra system (Shimadzu, Kyoto, Japan) equipped with Rxi-5MS capillary column (30 m × 0.25 mm; film thickness, 0.25 μm) (Restek, Bellefonte, PA, USA). The injector temperature was 250 °C, and the flow rate was 1 mL min−1. The column temperature was raised from 50 °C to 160 °C at a rate of 5 °C min−1, and then raised until 250 °C at a rate of 10 °C min−1. Split mode for samples injection was 1:20. Mass spectra were obtained at 220 °C, in electronic impact mode at 70 eV.

4.3. Antifungal Activity In Vitro

Antifungal activity against B. cinerea was determined by pouring clove and cinnamon extracts to PDA at concentrations of 200–2200 μL L−1. The research was carried out at the Laboratory of Plant Protection, LAMMC Institute of Horticulture, 2018–2019. Four replications were performed for the experiment. Inoculation was performed with a 6-mm plug of 7-day-old single spore isolate of B. cinerea, fungal side down. Isolates were obtained from infected strawberry fruit and had previously been identified as B. cinerea [45].
Petri dishes were incubated at 22 ± 2 °C in the dark for 7 days. The radial colony growth of the pathogen (plug excluded) was measured 2, 4 and 7 days after inoculation (DAI), and antifungal activity was expressed as the percentage inhibition of mycelial growth using the following formula [46]:
Inhibition (%) = (CT)/C × 100,
where C is the radial colony growth of the pathogen in control (mm); and T is the radial colony growth of the pathogen in treatment (mm).
The lowest concentration of cinnamon and clove extract with 100% inhibition was considered as minimal inhibitory concentration (MIC). Minimal fungicidal concentration (MFC) was determined after reinoculating B. cinerea treated with extracts from the margin of the colony on the fresh PDA. The lowest concentration with no visible radial colony growth of B. cinerea after 48 h was considered as MFC.

4.4. Antifungal Activity on Detached Strawberry Leaves

Detached strawberry leaves were soaked in 70% ethanol for 2 minutes and rinsed in sterile distilled water three times. Each leaf was placed in a Petri dish with filter paper and 5 mL of sterile distilled water. The mixtures for leaf treatment were prepared with sterile distilled water, 1% Tween 80 and cinnamon and clove extracts at 6 mL L−1 and 12 mL L−1. Concentrations were chosen and modified according to the obtained MICs of clove and cinnamon extracts. Detached strawberry leaves were sprayed with prepared mixtures, then wounded with a sterile needle and a 9-mm plug of 7-day-old B. cinerea was placed on the wound. Four replications were carried out. Incubation was carried out at 22 ± 2 °C in the dark for 7 days. Evaluations of lesion diameter were made 2, 4 and 7 days after inoculation, and inhibition of grey mould infection on detached strawberry leaves was counted using the formula referred to in Section 4.3, using lesion diameter instead of radial colony growth of the pathogen.

4.5. Statistical Analysis

SAS Enterprise Guide 7.1 program (SAS Institute Inc., Cary, NC, USA) was applied for the analysis of experimental data. Analysis of variance (ANOVA) procedure was processed, and Duncan’s multiple range test (p < 0.05) was used for the comparison of obtained means.

5. Conclusions

Extracts of cinnamon and clove, containing characteristic compounds trans-cinnamaldehyde and eugenol, showed significant antifungal activity against B. cinerea in vitro. The results showed that the antifungal activity of clove extract was stronger than that of the cinnamon extract at lower concentrations. Infection of grey mould on detached strawberry leaves was suppressed by the application of clove oil at the highest investigated concentration. The cinnamon extract was not that effective at inhibiting the spread of grey mould on strawberry leaves. The use of natural antifungal agents is being increasingly encouraged nowadays. Clove extract has high potential to be applied in the formulation of biopesticides for safer plant protection. Based on the in vitro results, further analysis of cinnamon extract is encouraged.

Author Contributions

Conceptualization: A.V.; methodology: L.Š., N.R. and A.V.; software: L.Š.; validation: N.R.; formal analysis: L.Š.; investigation: L.Š.; resources: N.R.; data curation: L.Š.; writing—original draft preparation: L.Š.; writing—review and editing: L.Š., N.R. and A.V.; visualization: L.Š.; supervision: A.V. All authors have read and agreed to the published version of the manuscript.


This research received no external funding.

Conflicts of Interest

The authors declare no conflict of interest.


  1. Mónaco, C.; Dal Bello, G.; Rollán, M.C.; Ronco, L.; Lampugnani, G.; Arteta, N.; Abramoff, C.; Aprea, A.; Larran, S.; Stocco, M. Biological control of Botrytis cinerea on tomato using naturally occurring fungal antagonists. Arch. Phytopathol. Plant Prot. 2009, 42, 729–737. [Google Scholar] [CrossRef]
  2. Daniel, C.K.; Lennox, C.L.; Vries, F.A. In vivo application of garlic extracts in combination with clove oil to prevent postharvest decay caused by Botrytis cinerea, Penicillium expansum and Neofabraea alba on apples. Postharvest Biol. Technol. 2015, 99, 88–92. [Google Scholar] [CrossRef]
  3. Rasiukevičiūtė, N.; Uselis, N.; Valiuškaitė, A. The use of forecasting model iMETOS® for strawberry grey mould management. Zemdirb. Agric. 2019, 106, 143–150. [Google Scholar] [CrossRef]
  4. Adrian, M.; Jeandet, P. Effects of resveratrol on the ultrastructure of Botrytis cinerea conidia and biological significance in plant/pathogen interactions. Fitoterapia 2012, 83, 1345–1350. [Google Scholar] [CrossRef]
  5. Elad, Y.; Vivier, M.; Fillinger, S. Botrytis, the good, the bad and the ugly. In Botrytis—The Fungus, the Pathogen and Its Management in Agricultural Systems; Fillinger, S., Elad, Y., Eds.; Springer International Publishing: Basel, Switzerland, 2016; pp. 1–15. ISBN 9783319233710. [Google Scholar] [CrossRef]
  6. Abbey, J.A.; Percival, D.; Abbey, L.; Asiedu, S.K.; Prithiviraj, B.; Schilder, A. Biofungicides as alternative to synthetic fungicide control of grey mould (Botrytis cinerea)–prospects and challenges. Biocontrol Sci. Technol. 2019, 29, 207–228. [Google Scholar] [CrossRef]
  7. Adebayo, O.; Dang, T.; Bélanger, A.; Khanizadeh, S. Antifungal studies of selected essential oils and a commercial formulation against Botrytis cinerea. J. Food Res. 2013, 2, 217–226. [Google Scholar] [CrossRef]
  8. Absalan, A.; Mohiti-Ardakani, J.; Hadinedoushan, H.; Khalili, M.A. Hydro-alcoholic cinnamon extract, enhances glucose transporter isotype-4 translocation from intracellular compartments into the cytoplasmic membrane of C2C12 myotubes. Indian J. Clin. Biochem. 2012, 27, 351–356. [Google Scholar] [CrossRef][Green Version]
  9. Rao, P.V.; Gan, S.H. Cinnamon: A multifaceted medicinal plant. Evid. Based Complement. Altern. Med. 2014, 2014, 642942. [Google Scholar] [CrossRef][Green Version]
  10. Hajimonfarednejad, M.; Ostovar, M.; Raee, M.J.; Hashempur, M.H.; Mayer, J.G.; Heydari, M. Cinnamon: A systematic review of adverse events. Clin. Nutr. 2019, 38, 594–602. [Google Scholar] [CrossRef]
  11. Nurmalasari, D.L.; Damiyanti, M.; Eriwati, Y.K. Effect of cinnamon extract solution on human tooth enamel surface roughness. J. Phys. Conf. Ser. 2018, 1073. [Google Scholar] [CrossRef]
  12. Soliman, M.M.; Hamid, O.M.A.; Maqsood, H.A.; Aziza, S.; El Sanosi, Y.; Ragab, O.A. Insulin-mimetic effects of cinnamon extract in wistar rats. Lucr. Stiint. Ser. Med. Vet. 2015, 55, 61–68. [Google Scholar]
  13. Ahmed, J.; Altun, E.; Aydogdu, M.O.; Gunduz, O.; Kerai, L.; Ren, G.; Edirisinghe, M. Anti-fungal bandages containing cinnamon extract. Int. Wound J. 2019, 16, 730–736. [Google Scholar] [CrossRef][Green Version]
  14. Cock, I.E.; Cheesman, M. Plants of the genus Syzygium (Myrtaceae): A review on ethnobotany, medicinal properties and phytochemistry. In Bioactive Compounds of Medicinal Plants: Properties and Potential for Human Health; Goyal, M.R., Ayeleso, A.O., Eds.; Apple Academic Press: Palm Bay, FL, USA, 2019; pp. 35–84. ISBN 9781771886482. [Google Scholar]
  15. Hamini-Kadar, N.; Hamdane, F.; Boutoutaou, R.; Kihal, M.; Henni, J.E. Antifungal activity of clove (Syzygium aromaticum L.) essential oil against phytopathogenic fungi of tomato (Solanum lycopersicum L.) in Algeria. J. Exp. Biol. Agric. Sci. 2014, 2, 447–454. [Google Scholar]
  16. Eman-Abdeen, E.; El-Diasty, E.M. Antifungal activity of clove oil on dermatophytes and other fungi. Int. J. Adv. Res. 2015, 3, 1299–1305. [Google Scholar]
  17. Božik, M.; Nový, P.; Klouček, P. Chemical composition & antimicrobial activity of cinnamon, thyme, oregano & clove essential oils against plant pathogenic bacteria. Acta Univ. Agric. Silvic. Mendel. Brun. 2017, 65, 1129–1134. [Google Scholar] [CrossRef][Green Version]
  18. Shabana, Y.M.; Abdalla, M.E.; Shahin, A.A.; El-Sawy, M.M.; Draz, I.S.; Youssif, A.W. Efficacy of plant extracts in controlling wheat leaf rust disease caused by Puccinia triticina. Egypt. J. Basic Appl. Sci. 2017, 4, 67–73. [Google Scholar] [CrossRef][Green Version]
  19. Šernaitė, L.; Valiuškaitė, A.; Rasiukevičiūtė, N.; Dambrauskienė, E.; Viškelis, P. The effect of spice extracts on strawberry pathogen Botrytis cinerea. In Proceedings of the 9th International Scientific Conference Rural Development 2019, Vytautas Magnus University Agriculture Academy, Akademija, Kaunas district, Lithuania, 26–28 September 2019; Raupelienė, A., Ed.; Vytautas Magnus University Agriculture Academy: Akademija, Kaunas district, Lithuania, 2019; pp. 79–83. [Google Scholar] [CrossRef]
  20. Combrinck, S.; Regnier, T.; Kamatou, G.P.P. In vitro activity of eighteen essential oils and some major components against common postharvest fungal pathogens of fruit. Ind. Crops Prod. 2011, 33, 344–349. [Google Scholar] [CrossRef]
  21. Gurjar, M.S.; Ali, S.; Akhtar, M.; Singh, K.S. Efficacy of plant extracts in plant disease management. Agric. Sci. 2012, 3, 425–433. [Google Scholar] [CrossRef][Green Version]
  22. Dayan, F.E.; Cantrell, C.L.; Duke, S.O. Natural products in crop protection. Bioorg. Med. Chem. 2009, 17, 4022–4034. [Google Scholar] [CrossRef]
  23. Antunes, M.D.C.; Cavaco, A.M. The use of essential oils for postharvest decay control. A review. Flavour Fragr. J. 2010, 25, 351–366. [Google Scholar] [CrossRef]
  24. Yazdani, F.; Mafi, M.; Farhadi, F.; Tabar-Heidar, K.; Aghapoor, K.; Mohsenzadeh, F.; Darabi, H.R. Supercritical CO2 extraction of essential oil from clove bud: Effect of operation conditions on the selective isolation of eugenol and eugenyl acetate. Z. Naturforsch. B J. Chem. Sci. 2005, 60, 1197–1201. [Google Scholar] [CrossRef]
  25. Rana, I.S.; Rana, A.S.; Rajak, R.C. Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol. Braz. J. Microbiol. 2011, 42, 1269–1277. [Google Scholar] [CrossRef]
  26. Oliveira, M.S.; Costa, W.A.; Pereira, D.S.; Botelho, J.R.S.; Menezes, T.O.A.; Andrade, E.H.A.; Silva, S.H.M.; Filho, A.P.S.S.; Carvalho Junior, R.N. Chemical composition and phytotoxic activity of clove (Syzygium aromaticum) essential oil obtained with supercritical CO2. J. Supercrit. Fluids 2016, 118, 185–193. [Google Scholar] [CrossRef]
  27. Moghadam, Z.A.; Hosseini, H.; Hadian, Z.; Asgari, B.; Mirmoghtadaie, L.; Mohammadi, A.; Shamloo, E.; Javadi, N.H.S. Evaluation of the antifungal activity of cinnamon, clove, thymes, Zataria multiflora, cumin and caraway essential oils against ochratoxigenic Aspergillus ochraceus. J. Pharm. Res. Int. 2019, 26, 1–16. [Google Scholar] [CrossRef]
  28. Xie, Y.; Yang, Z.; Cao, D.; Rong, F.; Ding, H.; Zhang, D. Antitermitic and antifungal activities of eugenol and its congeners from the flower buds of Syzygium aromaticum (clove). Ind. Crops Prod. 2015, 77, 780–786. [Google Scholar] [CrossRef]
  29. Wang, C.; Zhang, J.; Chen, H.; Yongjian, Y.; Shi, Z. Antifungal activity of eugenol against Botrytis cinerea. Trop. Plant Pathol. 2010, 35, 137–143. [Google Scholar] [CrossRef][Green Version]
  30. Olea, A.F.; Bravo, A.; Martínez, R.; Thomas, M.; Sedan, C.; Espinoza, L.; Zambrano, E.; Carvajal, D.; Silva-Moreno, E.; Carrasco, H. Antifungal activity of eugenol derivatives against Botrytis cinerea. Molecules 2019, 24, 1239. [Google Scholar] [CrossRef] [PubMed][Green Version]
  31. Melgarejo-Flores, B.G.; Ortega-Ramírez, L.A.; Silva-Espinoza, B.A.; González-Aguilar, G.A.; Miranda, M.R.A.; Ayala-Zavala, J.F. Antifungal protection and antioxidant enhancement of table grapes treated with emulsions, vapors, and coatings of cinnamon leaf oil. Postharvest Biol. Technol. 2013, 86, 321–328. [Google Scholar] [CrossRef]
  32. Park, J.Y.; Kim, S.H.; Kim, N.H.; Lee, S.W.; Jeun, Y.C.; Hong, J.K. Differential inhibitory activities of four plant essential oils on in vitro growth of Fusarium oxysporum f. sp. fragariae causing Fusarium wilt in strawberry plants. Plant Pathol. J. 2017, 33, 582–588. [Google Scholar] [CrossRef]
  33. Xie, Y.; Huang, Q.; Wang, Z.; Cao, H.; Zhang, D. Structure-activity relationships of cinnamaldehyde and eugenol derivatives against plant pathogenic fungi. Ind. Crops Prod. 2017, 97, 388–394. [Google Scholar] [CrossRef]
  34. Carmello, C.R.; Cardoso, J.C. Effects of plant extracts and sodium hypochlorite on lettuce germination and inhibition of Cercospora longissima in vitro. Sci. Hortic. 2018, 234, 245–249. [Google Scholar] [CrossRef]
  35. Zheng, J.; Liu, T.; Guo, Z.; Zhang, L.; Mao, L.; Zhang, Y.; Jiang, H. Fumigation and contact activities of 18 plant essential oils on Villosiclava virens, the pathogenic fungus of rice false smut. Sci. Rep. 2019, 9, 7330. [Google Scholar] [CrossRef] [PubMed][Green Version]
  36. Sharma, A.; Sharma, N.K.; Srivastava, A.; Kataria, A.; Dubey, S.; Sharma, S.; Kundu, B. Clove and lemongrass oil based non-ionic nanoemulsion for suppressing the growth of plant pathogenic Fusarium oxysporum f.sp. lycopersici. Ind. Crops Prod. 2018, 123, 353–362. [Google Scholar] [CrossRef]
  37. Haddi, K.; Faroni, L.R.A.; Oliveira, E.E. Cinnamon oil. In Green Pesticides Handbook: Essential Oils for Pest Control; Nollet, L.M.L., Rathore, H.S., Eds.; CRC Press: Boca Raton, FL, USA, 2017; pp. 117–150. ISBN 9781498759397. [Google Scholar] [CrossRef]
  38. Moussa, S.H.; Tayel, A.A.; Alsohim, A.S.; Abdallah, R.R. Botryticidal activity of nanosized silver-chitosan composite and its application for the control of gray mold in strawberry. J. Food Sci. 2013, 78, 1589–1594. [Google Scholar] [CrossRef]
  39. Pertot, I.; Zasso, R.; Amsalem, L.; Baldessari, M.; Angeli, G.; Elad, Y. Integrating biocontrol agents in strawberry powdery mildew control strategies in high tunnel growing systems. Crop Prot. 2008, 27, 622–631. [Google Scholar] [CrossRef]
  40. Nikolova, M.; Yordanov, P.; Slavov, S.; Berkov, S. Antifungal activity of some plant extracts against phytopathogenic fungi. J. Biosci. Biotechnol. 2017, 6, 155–161. [Google Scholar]
  41. Robinson-Boyer, L.; Jeger, M.J.; Xu, X.M.; Jeffries, P. Management of strawberry grey mould using mixtures of biocontrol agents with different mechanisms of action. Biocontrol Sci. Technol. 2009, 19, 1051–1065. [Google Scholar] [CrossRef]
  42. Xu, X.; Robinson, J.; Jeger, M.; Jeffries, P. Using combinations of biocontrol agents to control Botrytis cinerea on strawberry leaves under fluctuating temperatures. Biocontrol Sci. Technol. 2010, 20, 359–373. [Google Scholar] [CrossRef]
  43. Bajpai, S.; Shukla, P.S.; Asiedu, S.; Pruski, K.; Prithiviraj, B. A biostimulant preparation of brown seaweed Ascophyllum nodosum suppresses powdery mildew of strawberry. Plant Pathol. J. 2019, 35, 406–416. [Google Scholar] [CrossRef]
  44. Šernaitė, L.; Rasiukevičiūtė, N.; Dambrauskienė, E.; Viškelis, P.; Valiuškaitė, A. Efficacy of plant extracts and essential oils for biocontrol of strawberry pathogen Botrytis cinerea. Zemdirb. Agric. 2020, 107, 147–152. [Google Scholar] [CrossRef]
  45. Rasiukevičiūtė, N.; Rugienius, R.; Šikšnianienė, J.B. Genetic diversity of Botrytis cinerea from strawberry in Lithuania. Zemdirb. Agric. 2018, 105, 265–270. [Google Scholar] [CrossRef][Green Version]
  46. Cherkupally, R.; Kota, S.R.; Amballa, H.; Reddy, B.N. In vitro antifungal potential of plant extracts against Fusarium oxysporum, Rhizoctonia solani and Macrophomina phaseolina. Ann. Plant Sci. 2017, 6, 1676–1680. [Google Scholar] [CrossRef][Green Version]
Figure 1. Inhibition (%) of Botrytis cinerea mycelial growth by cinnamon (C. cassia) extract at 4 and 7 days after inoculation (4 DAI and 7 DAI). Results are presented as means (n = 4). The same letter indicates no significant differences between treatments (p < 0.05).
Figure 1. Inhibition (%) of Botrytis cinerea mycelial growth by cinnamon (C. cassia) extract at 4 and 7 days after inoculation (4 DAI and 7 DAI). Results are presented as means (n = 4). The same letter indicates no significant differences between treatments (p < 0.05).
Plants 09 00613 g001
Figure 2. Inhibition (%) of Botrytis cinerea mycelial growth by clove (S. aromaticum) extract at 4 and 7 days after inoculation (4 DAI and 7 DAI). Results are presented as means (n = 4). The same letter indicates no significant differences between treatments (p < 0.05).
Figure 2. Inhibition (%) of Botrytis cinerea mycelial growth by clove (S. aromaticum) extract at 4 and 7 days after inoculation (4 DAI and 7 DAI). Results are presented as means (n = 4). The same letter indicates no significant differences between treatments (p < 0.05).
Plants 09 00613 g002
Figure 3. Infection of grey mould on detached strawberry leaves 7 days after inoculation, after treatment with (a) cinnamon (C. cassia) extract; (b) clove (S. aromaticum) extract.
Figure 3. Infection of grey mould on detached strawberry leaves 7 days after inoculation, after treatment with (a) cinnamon (C. cassia) extract; (b) clove (S. aromaticum) extract.
Plants 09 00613 g003
Figure 4. Inhibition (%) of grey mould (Botrytis cinerea) by cinnamon (C. cassia) and clove (S. aromaticum) extracts on detached strawberry leaves at 4 and 7 days after inoculation (4 DAI and 7 DAI). Results are presented as means (n = 4). The same letter indicates no significant differences between treatments (p < 0.05).
Figure 4. Inhibition (%) of grey mould (Botrytis cinerea) by cinnamon (C. cassia) and clove (S. aromaticum) extracts on detached strawberry leaves at 4 and 7 days after inoculation (4 DAI and 7 DAI). Results are presented as means (n = 4). The same letter indicates no significant differences between treatments (p < 0.05).
Plants 09 00613 g004
Table 1. Composition of the volatile compounds of cinnamon (C. cassia) and clove (S. aromaticum) extracts. Results are presented as means (n = 3).
Table 1. Composition of the volatile compounds of cinnamon (C. cassia) and clove (S. aromaticum) extracts. Results are presented as means (n = 3).
Plant ExtractsCinnamomum cassiaSyzygium aromaticum
ComponentPA 1 (%)RT 2PA (%)RT
Chavicol 0.1415.983
α-cubebene 0.8218.163
Germacrene D 0.2721.568
trans-calamenene + eugenyl acetate + cadinene1.5922.584
Eugenol acetate 21.9522.822
Caryophyllene oxide0.6624.0990.5224.134
Methyl atrarate0.1327.423
Hexadecenoic acid0.6631.061
Squalene 0.5333.304
Other 31.40 1.35
Total identified98.86% 99.81%
1 PA—peak area. 2 RT—retention time. 3 Consists of compounds that were less than 0.1% of the quantity of the extract.
Table 2. Radial colony growth of Botrytis cinerea 48 h after reinoculation (after treatment with extracts). Results are presented as mean ± SD (n = 4) (p < 0.05).
Table 2. Radial colony growth of Botrytis cinerea 48 h after reinoculation (after treatment with extracts). Results are presented as mean ± SD (n = 4) (p < 0.05).
Plant ExtractsRadial Colony Growth (mm) at Different Concentrations in μL L−1
C. cassia34.8 ± 1.837.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.0
S. aromaticum4.0 ± 4.043.0 ± 1.521.8 ± 4.314.3 ± 2.215.0 ± 0.59.8 ± 1.80.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.0
Control39.3 ± 0.7

Share and Cite

MDPI and ACS Style

Šernaitė, L.; Rasiukevičiūtė, N.; Valiuškaitė, A. The Extracts of Cinnamon and Clove as Potential Biofungicides against Strawberry Grey Mould. Plants 2020, 9, 613.

AMA Style

Šernaitė L, Rasiukevičiūtė N, Valiuškaitė A. The Extracts of Cinnamon and Clove as Potential Biofungicides against Strawberry Grey Mould. Plants. 2020; 9(5):613.

Chicago/Turabian Style

Šernaitė, Lina, Neringa Rasiukevičiūtė, and Alma Valiuškaitė. 2020. "The Extracts of Cinnamon and Clove as Potential Biofungicides against Strawberry Grey Mould" Plants 9, no. 5: 613.

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop