Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress
Abstract
1. Introduction
2. Results
2.1. Cloning and Characterization of TPS and TPP cDNA
2.2. Amino Acid Sequence Analysis and Phylogenetic Relationship
2.3. In Silico Analysis of Catalytic Sites
2.4. Real-Time Quantitative PCR
3. Discussion
4. Materials and Methods
4.1. Plant Material, Growth Conditions and Stress Treatment
4.2. Watering Treatments
4.3. RNA Extraction and cDNA Synthesis
4.4. Cloning TPS/TPP cDNA and Sequence Analysis
4.5. Real-Time Quantitative PCR
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Acosta-Pérez, P.; Camacho-Zamora, B.D.; Espinoza-Sánchez, E.A.; Gutiérrez-Soto, G.; Zavala-García, F.; Abraham-Juárez, M.J.; Sinagawa-García, S.R. Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress. Plants 2020, 9, 315. https://doi.org/10.3390/plants9030315
Acosta-Pérez P, Camacho-Zamora BD, Espinoza-Sánchez EA, Gutiérrez-Soto G, Zavala-García F, Abraham-Juárez MJ, Sinagawa-García SR. Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress. Plants. 2020; 9(3):315. https://doi.org/10.3390/plants9030315
Chicago/Turabian StyleAcosta-Pérez, Phamela, Bianka Dianey Camacho-Zamora, Edward A. Espinoza-Sánchez, Guadalupe Gutiérrez-Soto, Francisco Zavala-García, María Jazmín Abraham-Juárez, and Sugey Ramona Sinagawa-García. 2020. "Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress" Plants 9, no. 3: 315. https://doi.org/10.3390/plants9030315
APA StyleAcosta-Pérez, P., Camacho-Zamora, B. D., Espinoza-Sánchez, E. A., Gutiérrez-Soto, G., Zavala-García, F., Abraham-Juárez, M. J., & Sinagawa-García, S. R. (2020). Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress. Plants, 9(3), 315. https://doi.org/10.3390/plants9030315