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Article

Alternative Polyadenylation and Salicylic Acid Modulate Root Responses to Low Nitrogen Availability

1
Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain
2
Centro de Biotecnología y Genómica de Plantas (CBGP) and Escuela Técnica Superior de Ingeniería Agronómica, Agroambiental y de Biosistemas (ETSIAAB), Universidad Polictécnica de Madrid, Campus de Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain
3
DTD Development and Technical Department, Timac Agro Spain, 31580 Lodosa, Navarra, Spain
4
Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546-0312, USA
5
Departamento de Biotecnología y Bioingeniería CINVESTAV Instituto Politécnico Nacional, 07360 Ciudad de Mexico, Mexico
6
Environmental Biology Department, University of Navarra, 31008 Navarra, Spain
*
Author to whom correspondence should be addressed.
Plants 2020, 9(2), 251; https://doi.org/10.3390/plants9020251
Received: 15 January 2020 / Revised: 11 February 2020 / Accepted: 13 February 2020 / Published: 16 February 2020
Nitrogen (N) is probably the most important macronutrient and its scarcity limits plant growth, development and fitness. N starvation response has been largely studied by transcriptomic analyses, but little is known about the role of alternative polyadenylation (APA) in such response. In this work, we show that N starvation modifies poly(A) usage in a large number of transcripts, some of them mediated by FIP1, a component of the polyadenylation machinery. Interestingly, the number of mRNAs isoforms with poly(A) tags located in protein-coding regions or 5′-UTRs significantly increases in response to N starvation. The set of genes affected by APA in response to N deficiency is enriched in N-metabolism, oxidation-reduction processes, response to stresses, and hormone responses, among others. A hormone profile analysis shows that the levels of salicylic acid (SA), a phytohormone that reduces nitrate accumulation and root growth, increase significantly upon N starvation. Meta-analyses of APA-affected and fip1-2-deregulated genes indicate a connection between the nitrogen starvation response and salicylic acid (SA) signaling. Genetic analyses show that SA may be important for preventing the overgrowth of the root system in low N environments. This work provides new insights on how plants interconnect different pathways, such as defense-related hormonal signaling and the regulation of genomic information by APA, to fine-tune the response to low N availability. View Full-Text
Keywords: root development; alternative polyadenylation; nitrogen starvation; salicylic acid root development; alternative polyadenylation; nitrogen starvation; salicylic acid
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MDPI and ACS Style

Conesa, C.M.; Saez, A.; Navarro-Neila, S.; de Lorenzo, L.; Hunt, A.G.; Sepúlveda, E.B.; Baigorri, R.; Garcia-Mina, J.M.; Zamarreño, A.M.; Sacristán, S.; del Pozo, J.C. Alternative Polyadenylation and Salicylic Acid Modulate Root Responses to Low Nitrogen Availability. Plants 2020, 9, 251. https://doi.org/10.3390/plants9020251

AMA Style

Conesa CM, Saez A, Navarro-Neila S, de Lorenzo L, Hunt AG, Sepúlveda EB, Baigorri R, Garcia-Mina JM, Zamarreño AM, Sacristán S, del Pozo JC. Alternative Polyadenylation and Salicylic Acid Modulate Root Responses to Low Nitrogen Availability. Plants. 2020; 9(2):251. https://doi.org/10.3390/plants9020251

Chicago/Turabian Style

Conesa, Carlos M., Angela Saez, Sara Navarro-Neila, Laura de Lorenzo, Arthur G. Hunt, Edgar B. Sepúlveda, Roberto Baigorri, Jose M. Garcia-Mina, Angel M. Zamarreño, Soledad Sacristán, and Juan C. del Pozo 2020. "Alternative Polyadenylation and Salicylic Acid Modulate Root Responses to Low Nitrogen Availability" Plants 9, no. 2: 251. https://doi.org/10.3390/plants9020251

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