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RING-Type E3 Ubiqitin Ligase Barley Genes (HvYrg1–2) Control Characteristics of Both Vegetative Organs and Seeds as Yield Components

1
Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62., H-6726 Szeged, Hungary
2
Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary
3
Laboratory of Cellular Imaging, Biological Research Center, Temesvári krt. 62., H-6726 Szeged, Hungary
4
Cereal Research Non-Profit Ltd., Alsó Kikötő Sor 9., H-6726 Szeged, Hungary
5
Institute of Genetics, Biological Research Centre, Temesvári krt. 62., H-6726 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Equal contribution.
Plants 2020, 9(12), 1693; https://doi.org/10.3390/plants9121693
Received: 13 November 2020 / Revised: 26 November 2020 / Accepted: 30 November 2020 / Published: 2 December 2020
Previously, studies on RING-type E3 ubiquitin ligases in cereals were preferentially focused on GW2 genes primarily controlling seed parameters in rice and wheat. Here we report cloning two HvYrg genes from barley that share significant homology with rice GW2 gene. In antisense genotypes efficiency of gene silencing varied between genes and transgenic lines: ASHvYrg1: 30–50% and ASHvYrg2: 20–27%. Reduced activity of both genes altered shoot system with increasing number of side shoots. Changes in leaf width, weight, or plant weight and height reached significant levels in some transgenic lines. Lowering expression of the two barley HvYrg genes caused opposite responses in spike development. Plants with ASHvYrg1 gene construct showed earlier heading time and prolonged grain-filling period, while plants from ASHvYrg2 genotype flowered in delay. Digital imaging of root development revealed that down-regulation of HvYrg1 gene variant stimulated root growth, while ASHvYrg2 plants developed reduced root system. Comparison of seed parameters indicated an increase in thousand grain weight accompanied with longer and wider seed morphology. In summary we conclude that in contrast to inhibition of GW2 genes in rice and wheat plants, down-regulation of the barely HvYrg genes caused substantial changes in vegetative organs in addition to alteration of seed parameters. View Full-Text
Keywords: Hordeum vulgare L.; HvYrg gene variants; antisense; down regulation; shoot system; heading time; seed size; seed morphology; root development; digital imaging Hordeum vulgare L.; HvYrg gene variants; antisense; down regulation; shoot system; heading time; seed size; seed morphology; root development; digital imaging
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MDPI and ACS Style

Zombori, Z.; Nagy, B.; Mihály, R.; Pauk, J.; Cseri, A.; Sass, L.; Horváth V., G.; Dudits, D. RING-Type E3 Ubiqitin Ligase Barley Genes (HvYrg1–2) Control Characteristics of Both Vegetative Organs and Seeds as Yield Components. Plants 2020, 9, 1693. https://doi.org/10.3390/plants9121693

AMA Style

Zombori Z, Nagy B, Mihály R, Pauk J, Cseri A, Sass L, Horváth V. G, Dudits D. RING-Type E3 Ubiqitin Ligase Barley Genes (HvYrg1–2) Control Characteristics of Both Vegetative Organs and Seeds as Yield Components. Plants. 2020; 9(12):1693. https://doi.org/10.3390/plants9121693

Chicago/Turabian Style

Zombori, Zoltán; Nagy, Bettina; Mihály, Róbert; Pauk, János; Cseri, András; Sass, László; Horváth V., Gábor; Dudits, Dénes. 2020. "RING-Type E3 Ubiqitin Ligase Barley Genes (HvYrg1–2) Control Characteristics of Both Vegetative Organs and Seeds as Yield Components" Plants 9, no. 12: 1693. https://doi.org/10.3390/plants9121693

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