RNA-Seq Time Series of Vitis vinifera Bud Development Reveals Correlation of Expression Patterns with the Local Temperature Profile

Pucker, Boas; Schwandner, Anna; Becker, Sarah; Hausmann, Ludger; Viehöver, Prisca; Töpfer, Reinhard; Weisshaar, Bernd; Holtgräwe, Daniela

doi:10.3390/plants9111548

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RNA-Seq Time Series of Vitis vinifera Bud Development Reveals Correlation of Expression Patterns with the Local Temperature Profile

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Genetics and Genomics of Plants, Faculty of Biology and Center for Biotechnology (CeBiTec), Bielefeld University, 33615 Bielefeld, Germany

Evolution and Diversity, Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK

Julius Kühn-Institut (JKI), Institute for Grapevine Breeding Geilweilerhof, 76833 Siebeldingen, Germany

Author to whom correspondence should be addressed.

Plants 2020, 9(11), 1548; https://doi.org/10.3390/plants9111548

Received: 17 October 2020 / Revised: 8 November 2020 / Accepted: 9 November 2020 / Published: 12 November 2020

(This article belongs to the Section Plant Molecular Biology)

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Abstract

Plants display sophisticated mechanisms to tolerate challenging environmental conditions and need to manage their ontogenesis in parallel. Here, we set out to generate an RNA-Seq time series dataset throughout grapevine (Vitis vinifera) early bud development. The expression of the developmental regulator VviAP1 served as an indicator of the progression of development. We investigated the impact of changing temperatures on gene expression levels during the time series and detected a correlation between increased temperatures and a high expression level of genes encoding heat-shock proteins. The dataset also allowed the exemplary investigation of expression patterns of genes from three transcription factor (TF) gene families, namely MADS-box, WRKY, and R2R3-MYB genes. Inspection of the expression profiles from all three TF gene families indicated that a switch in the developmental program takes place in July which coincides with increased expression of the bud dormancy marker gene VviDRM1. View Full-Text

Keywords: grapevine; ontogenesis; gene expression; AP1; DRM1; MYB; WRKY; MADS-box; HSP; heat-shock genes; qRT-PCR reference genes

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MDPI and ACS Style

Pucker, B.; Schwandner, A.; Becker, S.; Hausmann, L.; Viehöver, P.; Töpfer, R.; Weisshaar, B.; Holtgräwe, D. RNA-Seq Time Series of Vitis vinifera Bud Development Reveals Correlation of Expression Patterns with the Local Temperature Profile. Plants 2020, 9, 1548. https://doi.org/10.3390/plants9111548

AMA Style

Pucker B, Schwandner A, Becker S, Hausmann L, Viehöver P, Töpfer R, Weisshaar B, Holtgräwe D. RNA-Seq Time Series of Vitis vinifera Bud Development Reveals Correlation of Expression Patterns with the Local Temperature Profile. Plants. 2020; 9(11):1548. https://doi.org/10.3390/plants9111548

Chicago/Turabian Style

Pucker, Boas, Anna Schwandner, Sarah Becker, Ludger Hausmann, Prisca Viehöver, Reinhard Töpfer, Bernd Weisshaar, and Daniela Holtgräwe. 2020. "RNA-Seq Time Series of Vitis vinifera Bud Development Reveals Correlation of Expression Patterns with the Local Temperature Profile" Plants 9, no. 11: 1548. https://doi.org/10.3390/plants9111548

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RNA-Seq Time Series of Vitis vinifera Bud Development Reveals Correlation of Expression Patterns with the Local Temperature Profile

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