A Cytosolic Protein Kinase STY46 in Arabidopsis thaliana Is Involved in Plant Growth and Abiotic Stress Response
Abstract
1. Introduction
2. Results
2.1. In Silico Sequence and Expression Analysis of STY46
2.2. Expression Analysis of STY46 in Arabidopsis thaliana Rosettes
2.3. Generation of STY46 Transgenic Plants
2.4. Characterization of STY46 Transgenic Plants under Control Conditions
2.5. Characterization of STY46 Transgenic Plants under Stress
2.6. 14CO2 Partitioning and Allocation in Stress-Treated Plant Tissues
3. Discussion
3.1. Expression Analysis of STY46 under Stressed Conditions
3.2. STY46 Has a Role in Regulating Growth of Arabidopsis Source and Sink Tissues
3.3. The Role of STY46 in Abiotic Stress Response
4. Materials and Methods
4.1. Analysis of STY46 T-DNA Mutant Lines
4.2. Generation of STY46 Overexpressing Transgenic Arabidopsis Lines
4.3. Stress Treatments
4.4. Genomic DNA Extraction
4.5. Quantitative Real-Time Reverse Transcript-PCR
4.6. Carbohydrate Analysis
4.7. 14CO2 Pulse-Chase Labeling and Fractionation of 14CO2-Labelled Plant Tissue
4.8. Photosynthesis Measurements
4.9. Assay of Rosette Size and Biomass
4.10. Assay of Seed Size and Seed Weight
4.11. Total Protein Extraction and Quantification
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Dong, S.; Zhang, F.; Beckles, D.M. A Cytosolic Protein Kinase STY46 in Arabidopsis thaliana Is Involved in Plant Growth and Abiotic Stress Response. Plants 2020, 9, 57. https://doi.org/10.3390/plants9010057
Dong S, Zhang F, Beckles DM. A Cytosolic Protein Kinase STY46 in Arabidopsis thaliana Is Involved in Plant Growth and Abiotic Stress Response. Plants. 2020; 9(1):57. https://doi.org/10.3390/plants9010057
Chicago/Turabian StyleDong, Shaoyun, Fenglan Zhang, and Diane M. Beckles. 2020. "A Cytosolic Protein Kinase STY46 in Arabidopsis thaliana Is Involved in Plant Growth and Abiotic Stress Response" Plants 9, no. 1: 57. https://doi.org/10.3390/plants9010057
APA StyleDong, S., Zhang, F., & Beckles, D. M. (2020). A Cytosolic Protein Kinase STY46 in Arabidopsis thaliana Is Involved in Plant Growth and Abiotic Stress Response. Plants, 9(1), 57. https://doi.org/10.3390/plants9010057