Investigating the Side-Effects of Neem-Derived Pesticides on Commercial Entomopathogenic and Slug-Parasitic Nematode Products Under Laboratory Conditions
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Preparation of Neem Leaf Extract (NLE)
4.2. Preparation of Azadirachtin (NA)
4.3. Entomopathogenic (EPN) and Slug-Parasitic (SPN) Nematodes
4.4. Experimental Setup
4.5. Data Elaboration and Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
- Mondal, D.; Mondal, T. A review of efficacy of Azadirachta indica A. Juss based biopesticides: An Indian perspective. Res. J. Recent Sci. 2012, 1, 94–99. [Google Scholar]
- Mahmoud, M.F. Combining the botanical insecticides NSK extract, NeemAzal T 5%, Neemix 4.5% and the entomopathogenic nematode Steinernema feltiae Cross N 33 to control the peach fruit fly, Bactrocera zonata (Saunders). Plant Protect. Sci 2007, 43, 19–25. [Google Scholar] [CrossRef]
- Otieno, J.A.; Pallmann, P.; Poehling, H.-M. The combined effect of soil-applied azadirachtin with entomopathogens for integrated management of western flower thrips. J. Appl. Entomol. 2015, 140, 174–186. [Google Scholar] [CrossRef]
- Schmutterer, H. Potential of azadirachtin-containing pesticides for integrated pest control in developing and industrialized countries. J. Insect Physiol. 1988, 34, 713–719. [Google Scholar] [CrossRef]
- Akhtar, M. Nematicidal potential of the neem tree Azadirachta indica (A. Juss). Integrated Pest Manag. Rev. 2000, 5, 57–66. [Google Scholar] [CrossRef]
- Trifonova, Z.; Atanasov, A. Control of potato cyst nematode Globodera rostochiensis with some plant extracts and neem products. Bulg. J. Agric. Sci. 2011, 17, 623–627. [Google Scholar]
- Silva, J.C.T.; Oliveira, R.D.L.; Jham, G.N.; Aguiar, N.D.C. Effect of neem seed extracts on the development of the Soybean Cysts Nematode. Trop. Plant Pathol. 2008, 33, 171–179. [Google Scholar] [CrossRef] [Green Version]
- Javed, N.; Gowen, S.R.; El-Hassan, S.A.; Inam-ul-Haq, M.; Shahina, F.; Pembroke, B. Efficacy of neem (Azadirachta indica) formulations on biology of root-knot nematodes (Meloidogyne javanica) on tomato. Crop Prot. 2008, 27, 36–43. [Google Scholar] [CrossRef]
- Meyer, J.; Ebssa, L.; Poehling, H.M. Effects of NeemAzal-U on survival, host infestation and reproduction of entomopathogenic and plant-parasitic nematodes: Heterorhabditis bacteriophora and Meloidogyne incognita. J. Plant Dis. Prot. 2012, 119, 142–151. [Google Scholar] [CrossRef]
- Saravanapriya, B.; Sivakumar, M. Management of root knot nematode Meloidogyne incognita on tomato with botanicals. Nat. Prod. Rad. 2005, 4, 158–161. [Google Scholar]
- Langat, J.K.; Kimenju, J.W.; Mutua, G.K.; Muiru, W.M.; Otieno, W. Response of free-living nematodes to treatments targeting plant parasitic nematodes in carnation. Asian J. Plant Sci. 2008, 7, 467–472. [Google Scholar] [CrossRef]
- Barzman, M.; Bàrberi, P.; Birch, N.; Boonekamp, P.; Dachbrodt-Saaydeh, S.; Graf, B.; Hommel, B.; Jensen, J.E.; Kiss, J.; Kudsk, P.; et al. Eight principles of integrated pest management. Agron. Sustain. Dev. 2015, 35, 1199. [Google Scholar] [CrossRef]
- Askary, T.H.; Abd-Elgawad, M.M.M. Beneficial nematodes in agroecosystems: A global perspective. In Biocontrol Agents: Entomopathogenic and Slug Parasitic Nematodes; Abd-Elgawad, M.M.M., Askary, T.H., Coupland, J., Eds.; CAB International: Wallingford, UK, 2017; pp. 3–25. [Google Scholar]
- Jagdale, G.B.; Kamoun, S.; Grewal, P.S. Entomopathogenic nematodes induce components of systemic resistance in plants: Biochemical and molecular evidence. Biol. Control 2009, 51, 102–109. [Google Scholar] [CrossRef]
- Wilson, M.J.; Glen, D.M.; George, S.K. The rhabditid nematode Phasmarhabditis hermaphrodita as a potential biological control agent for slugs. Biocontrol Sci. Technol. 1993, 3, 503–511. [Google Scholar] [CrossRef]
- Askary, T.H.; Khan, A.A.; Waliullah, M.I.S.; Banday, S.A.; Iqbal, U.; Mir, M.M. Slug pest management through nematodes in agricultural and horticultural crops. In Nematodes: Morphology, Functions and Management Strategies; Boeri, F., Chung, J.A., Eds.; Nova Publishers: New York, NY, USA, 2012; pp. 197–211. [Google Scholar]
- Lacey, L.A.; Georgis, R. Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial production. J. Nematol. 2012, 44, 218–225. [Google Scholar]
- Stark, J.D. Entomopathogenic nematodes (Rhabditida: Steinernematidae): Toxicity of neem. Ecotoxicology 1996, 89, 68–73. [Google Scholar] [CrossRef]
- Krishnayya, P.V.; Grewal, P.S. Effect of neem and selected fungicides on viability and virulence of the entomopathogenic nematode Steinernema feltiae. Biocontrol Sci. Technol. 2002, 12, 259–266. [Google Scholar] [CrossRef]
- Laznik, Ž.; Trdan, S. The influence of insecticides on the viability of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) under laboratory conditions. Pest Manag. Sci. 2013, 70, 784–789. [Google Scholar] [CrossRef]
- West, A.J.; Luntz, A.J.M. The influence of azadirachtin on the feeding behaviour of cereal aphids and slugs. Entomol. Exp. Appl. 1992, 62, 75–79. [Google Scholar] [CrossRef]
- Ploomi, A.; Jõgar, K.; Metspalu, L.; Hiiesaar, K.; Loorits, L.; Sibul, I.; Kivimägi, I.; Luik, A. The toxicity of Neem to the snail Arianta arbustorum. Sodininkystė ir Daržininkystė 2009, 28, 153–158. [Google Scholar]
- Laznik, Ž.; Vidrih, M.; Trdan, S. The effects of different fungicides on the viability of entomopathogenic nematodes Steinernema feltiae (Filipjev), S. carpocapsae Weiser, and Heterorhabditis downesi Stock, Griffin & Burnell (Nematoda: Rhabditida) under laboratory conditions. Chil. J. Agric. Res. 2012, 72, 62–67. [Google Scholar]
- Abdel-Razek, A.S.; Gowen, S. The integrated effect of the nematode-bacteria complex and neem plant extracts against Plutella xylostella (L.) larvae (Lepidoptera: Yponomeutidae) on chinese cabbage. Arch. Phytopathol. Plant Protect. 2002, 35, 181–188. [Google Scholar] [CrossRef]
- Sadeghian, M.M.; Mortazaienezhad, F. Investigation of compounds from Azadirachta indica (neem). Asian J. Plant Sci. 2007, 6, 444–445. [Google Scholar] [CrossRef]
- Mojumder, V.; Kamra, A.; Dureja, P. Effect of neem extracts on activity and mortality of second-stage juveniles of Meloidogyne incognita. Nematol. Medit. 2002, 30, 83–84. [Google Scholar]
- NIIR Board of Consultants & Engineers. HandBook on Neem & Allied Products; Asia Pacific Press: Delhi, India, 2004; p. 478. [Google Scholar]
- Chen, L.; Han, B.; Peng, L. Bioactivity of the neem seed kernel supercritical extracts towards Tribolium castaneum (Herbst). J. Food Agric. Environ. 2012, 10, 1511–1514. [Google Scholar] [CrossRef]
- Doshi, P.; Mészárosné Póss, A.; Tóth, F.; Szalai, M.; Turóczi, G. Effect of neem-derived plant protection products on the isopod species Porcellionides pruinosus (Brandt, 1833). ZooKeys 2018, 801, 415–425. [Google Scholar] [CrossRef]
- Nébih. Available online: https://novenyvedoszer.nebih.gov.hu/Engedelykereso/DocumentHandler.ashx?documentId=8a8082a8463a37810146f77dbaba6f26&documentName=NeemAzalTS_mod_kiskult_20140429.pdf (accessed on 5 August 2019).
- Ciancio, A. Observations on the nematicidal properties of some mycotoxins. Fundam. Appl. Nematol. 1995, 18, 451–454. [Google Scholar]
- Kiss, L.V.; Hrács, K.; Nagy, P.I.; Seres, A. Effects of zinc oxide nanoparticles on Panagrellus redivivus (Nematoda) and Folsomia candida (Collembola) in various test media. Int. J. Environ. Res. 2018, 12, 233–243. [Google Scholar] [CrossRef]
- Hammer, Ø.; Harper, D.A.T.; Ryan, P.D. PAST: Paleontological statistics software package for education and data analysis. Palaeontol. Electron. 2001, 4, 1–9. [Google Scholar] [CrossRef]
Species | LC10 | LC50 |
---|---|---|
H. bacteriophora | 0.179% | 0.217% |
Ph. hermaphrodita | 0.132% | 0.366% |
S. carpocapsae | 0.293% | 0.330% |
S. feltiae | 0.172% | 0.480% |
S. kraussei | 0.185% | 0.313% |
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Petrikovszki, R.; Doshi, P.; Turóczi, G.; Tóth, F.; Nagy, P. Investigating the Side-Effects of Neem-Derived Pesticides on Commercial Entomopathogenic and Slug-Parasitic Nematode Products Under Laboratory Conditions. Plants 2019, 8, 281. https://doi.org/10.3390/plants8080281
Petrikovszki R, Doshi P, Turóczi G, Tóth F, Nagy P. Investigating the Side-Effects of Neem-Derived Pesticides on Commercial Entomopathogenic and Slug-Parasitic Nematode Products Under Laboratory Conditions. Plants. 2019; 8(8):281. https://doi.org/10.3390/plants8080281
Chicago/Turabian StylePetrikovszki, Renáta, Pratik Doshi, György Turóczi, Ferenc Tóth, and Péter Nagy. 2019. "Investigating the Side-Effects of Neem-Derived Pesticides on Commercial Entomopathogenic and Slug-Parasitic Nematode Products Under Laboratory Conditions" Plants 8, no. 8: 281. https://doi.org/10.3390/plants8080281