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Open AccessFeature PaperArticle

Effects of Jasmonate on Ethylene Function during the Development of Tomato Stamens

Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, D06120 Halle (Saale), Germany
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Author to whom correspondence should be addressed.
Plants 2019, 8(8), 277; https://doi.org/10.3390/plants8080277
Received: 28 June 2019 / Revised: 16 July 2019 / Accepted: 6 August 2019 / Published: 9 August 2019
(This article belongs to the Special Issue The Jasmonate Pathway: New Actors, Mechanisms and Impacts)
The phenotype of the tomato mutant jasmonate-insensitive1-1 (jai1-1) mutated in the JA-Ile co-receptor COI1 demonstrates JA function in flower development, since it is female-sterile. In addition, jai1-1 exhibits a premature anther dehydration and pollen release, being in contrast to a delayed anther dehiscence in the JA-insensitive Arabidopsis mutant coi1-1. The double mutant jai1-1 Never ripe (jai1-1 Nr), which is in addition insensitive to ethylene (ET), showed a rescue of the jai1-1 phenotype regarding pollen release. This suggests that JA inhibits a premature rise in ET to prevent premature stamen desiccation. To elucidate the interplay of JA and ET in more detail, stamen development in jai1-1 Nr was compared to wild type, jai1-1 and Nr regarding water content, pollen vitality, hormone levels, and accumulation of phenylpropanoids and transcripts encoding known JA- and ET-regulated genes. For the latter, RT-qPCR based on nanofluidic arrays was employed. The data showed that additional prominent phenotypic features of jai1-1, such as diminished water content and pollen vitality, and accumulation of phenylpropanoids were at least partially rescued by the ET-insensitivity. Hormone levels and accumulation of transcripts were not affected. The data revealed that strictly JA-regulated processes cannot be rescued by ET-insensitivity, thereby emphasizing a rather minor role of ET in JA-regulated stamen development. View Full-Text
Keywords: ethylene; gene expression; jasmonate; nanofluidic arrays; phenylpropanoids; pollen vitality; Solanum lycopersicum (tomato); stamen development ethylene; gene expression; jasmonate; nanofluidic arrays; phenylpropanoids; pollen vitality; Solanum lycopersicum (tomato); stamen development
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Schubert, R.; Grunewald, S.; von Sivers, L.; Hause, B. Effects of Jasmonate on Ethylene Function during the Development of Tomato Stamens. Plants 2019, 8, 277.

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