Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (Vigna vexillata) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance
Abstract
:1. Introduction
2. Results and Discussion
2.1. Anatomical and Morphological Changes of Zombi Pea Varieties Subjected to Waterlogging Stress
2.2. De Novo Transcriptome Analysis
2.3. Differential Gene Expression, Functional Enrichment, and Comparative Transcriptome Analyses
2.4. Waterlogging Resulted in Stronger Induction of Glycolysis and Fermentative Genes in “Bali” than in “A408”
2.5. Waterlogging Resulted in Stronger Induction of Ethylene Synthesis, Perception and Responsive Genes in “Bali” than in “A408”
2.6. Auxin Metabolism and Auxin-Regulated Transcription Factor Genes were Predominantly Induced in the Roots of Waterlogging-Tolerant Zombi Pea
2.7. Differential Expression of Abscisic Acid and Gibberellic Acid Metabolic Genes
2.8. Differential Expression of Transport Genes
2.9. Differential Expression of Plant Cell Wall-Related Genes
2.10. Validation of Transcriptome Data by Quantitative Real-Time PCR
3. Material and Methods
3.1. Plant Material and Stress Treatment
3.2. Analysis of Leaf Chlorophyll Content
3.3. Root Anatomical and Morphological Analysis
3.4. Analysis of Total Soluble Carbohydrate Content
3.5. RNA Extraction, Library Preparation, and Sequencing
3.6. De Novo Assembly and Annotation
3.7. Differential Gene Expression Analysis
3.8. Quantitative Real-Time PCR
4. Conclusions
Supplementary Materials
Author contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Butsayawarapat, P.; Juntawong, P.; Khamsuk, O.; Somta, P. Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (Vigna vexillata) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance. Plants 2019, 8, 264. https://doi.org/10.3390/plants8080264
Butsayawarapat P, Juntawong P, Khamsuk O, Somta P. Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (Vigna vexillata) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance. Plants. 2019; 8(8):264. https://doi.org/10.3390/plants8080264
Chicago/Turabian StyleButsayawarapat, Pimprapai, Piyada Juntawong, Ornusa Khamsuk, and Prakit Somta. 2019. "Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (Vigna vexillata) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance" Plants 8, no. 8: 264. https://doi.org/10.3390/plants8080264