Genetic Structure of Cultivated Varieties of Radicchio (Cichorium intybus L.): A Comparison between F1 Hybrids and Synthetics
Abstract
:1. Introduction
2. Results
2.1. Genetic Structure of Commercial Open Pollinated (OP) Varieties, F1 Hybrids and their F2 Progenies
2.2. Genomic Comparison among Three Years Production of An F1 Hybrid Variety
3. Discussion
3.1. Genetic Structure of A Core Collection of OP, F1 and F2 Populations
3.2. Genomic Comparison among Three Different Production Years of A Commercial F1 Hybrid
4. Materials and Methods
4.1. Plant Materials and DNA Isolation
4.2. Genotyping by SSR Markers
4.3. Genetic Structure of Populations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Accession ID | No. of Individuals | Population Type | Varietal Cycles (d) |
---|---|---|---|
OP-1 | 30 | OP | 70 |
OP-2 | 30 | OP | 110 |
F1-A | 30 | F1 | 70 |
F1-B | 30 | F1 | 110 |
F1-C | 18 | F1 | 100 |
F1-D | 18 | F1 | 70 |
F2-C | 30 | F2 | 100 |
F2-D | 30 | F2 | 70 |
ID | OPs | F1s | F2s | |||
---|---|---|---|---|---|---|
Na | Ne | Na | Ne | Na | Ne | |
Mean | 4.5 | 2.6 | 2.3 | 1.8 | 2.8 | 1.9 |
St. Dev. | 2.4 | 1.2 | 0.9 | 0.6 | 0.9 | 0.5 |
ID | LG | Motif | Primer Sequence and Tail | Multiplex | |
---|---|---|---|---|---|
M1.1 | 1 | (GA)40 | F | [PAN3]CCAACGGATACCAAGGTGTT | 1 |
R | AACCGCACGGGTTCTATG | ||||
M2.4 | 2 | (GA)25 | F | [M13]CCGCTCTCTCATCACTCCTC | 1 |
R | GCTCGAAAATCGGCTACAAC | ||||
M2.5 | 2 | (CT)5CC(CT)13 TT(CT)5 | F | [PAN1]GTGCCGGTCTTCAGGTTACA | 1 |
R | CGCCTACCGATTACGATTGA | ||||
M3.7 | 3 | (CT)22 | F | TTCGAGTCTTGCCTTAATTGTT | 1 |
R | [PAN1]CAGACGACCTTACGGCAACT | ||||
M4.10a | 4 | (CT)22 | F | [PAN2]CATCACCTTCACGAAAAGCA | 1 |
R | CGAAGACCATCCATCACCA | ||||
M4.10b | 4 | (CT)21CATA(CA)5CT(CA)5 | F | [M13] CCATTATTGGGCAGCA | 1 |
R | CACCAACGAACTCCTTACAAAG | ||||
M4.11a | 4 | (CT)12N5(CA)11 | F | [PAN3] GAAGGAACCTATGAACCAACCACTCA | 1 |
R | GTTTTGAGCCTGAGCCAGA | ||||
M5.15 | 5 | (CT)11N7(CAA)5 | F | AGCACGACTCTGCTGTCTTTT | 1 |
R | [PAN1]CGAGCCATGTTAGGGTTTGT | ||||
M8.22 | 8 | (CA)5AA(CA)9 | F | [PAN1]TCGTCATCAGAAACAAAGCAA | 1 |
R | CAAAGAAGGCACTCTTGTCG | ||||
M9.26 | 9 | (GATA)3N19(GA)9 | F | [PAN2]CCTACACTCGGCCACCTACT | 1 |
R | TCGACGGTATAACAACACCTG | ||||
M3.8 | 3 | (CT)16 | F | [PAN1]AGGAAGCGGTGTCATCTGT | 2 |
R | CGCCCACATATTCATTCTCA | ||||
M6.16 | 6 | (CT)12TT(CT)15TT(CT)2TT(CT)4 | F | [PAN1]TATTGCATTGTTGTTCCTTG | 2 |
R | TATTTAGAAGAGGGAAATAGATG | ||||
M7.19 | 7 | (CT)18 | F | ATGTCGGAGCAAAATCGTTC | 2 |
R | [PAN1]CATGTTCCCGCTCATGAATA | ||||
M1.2 | 7 | (CT)19 | F | CCGGCAGAATTTTTAGGG | 2 |
R | [PAN3]CAGGTCATAGGTCCATGTGAAA | ||||
M1.3 | 1 | (CT)17 | F | [PAN3]TGGAGAAAAATGAAGCAC | 2 |
R | GAATGAGTGAGAGAATGATAGGG | ||||
M5.13 | 5 | (CT)23 | F | [M13]AGGCATAAAGAGGTGTGG | 2 |
R | TCAAACATGAAAACCGCTC | ||||
M6.17 | 6 | (CA)8(CT)18 | F | CGTGTCCAAACGCAAACATTAT | 2 |
R | [PAN2]GCACAATTTTCCTACCACTTATCC | ||||
M5.14 | 5 | (TC)11 | F | [M13]AAAGTCACACATCGCATTTCCT | 2 |
R | GTAGCAGCAGCAGCCATCTT | ||||
M4.11b | 4 | (TG)5CG(TG)7 | F | [M13]GCCATTCCTTTCAAGAGCAG | 2 |
R | AACCCAAAACCGCAACAATA | ||||
M4.12 | 4 | (CT)8TT(CT)5CC(CT)3TT(CT)7 | F | GGCATCGGGATAGAAAAACA | 2 |
R | [PAN2]TCAATGCCTCAACAGAAATCC | ||||
M3.9 | 3 | (CA)12 | F | CTGCTATGGACAGTTCCAGT | 3 |
R | [PAN3]CAATTCAGTTGTGATAGACGC | ||||
M7.20 | 7 | (CT)31 | F | [PAN2]ACACTCACTCACACTCCGTAA | 3 |
R | GTCATGATGGCGTAAAAGTC | ||||
M8.23 | 8 | (CA)11(CT)9 | F | TGTAGACACACAAAATGCACA | 3 |
R | [M13]ACCGGTTGAAAACATGAAAT | ||||
M8.24 | 8 | (TC)16(CA)13 | F | [PAN2]GGTCCGTAGACTGCAGACTTTT | 3 |
R | CACCGTCCCACTTTTTAGG | ||||
M9.25 | 9 | (CA)11 | F | [M13]GTGTGGGTGTTTGAAGAGC | 3 |
R | TCAAGAACATCAACGCGTAA | ||||
M7.21 | 1 | (CT)13 | F | GGACACCGAGCTGGAGAA | 3 |
R | [PAN1]TTCCACTTTCGGGAGTTACC | ||||
M9.27 | 9 | (GA)10TAAA(GA)5 | F | GCTAAAAGAAGTGCAAGGAGA | 3 |
R | [PAN1]TGTTCTTTCAAGTGCCAA | ||||
M6.18 | 6 | (CT)16 | F | [PAN3]CTCAACGAATGCTTTGGACA | 3 |
R | CCTCGCGGTAGCTTATTGTT | ||||
M2.6 | 2 | (CT)26 | F | GGAGCAGGTAGAGTCCCATC | 3 |
R | [PAN1]CGTTTGAAAATTTATACCAAAATG |
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Patella, A.; Scariolo, F.; Palumbo, F.; Barcaccia, G. Genetic Structure of Cultivated Varieties of Radicchio (Cichorium intybus L.): A Comparison between F1 Hybrids and Synthetics. Plants 2019, 8, 213. https://doi.org/10.3390/plants8070213
Patella A, Scariolo F, Palumbo F, Barcaccia G. Genetic Structure of Cultivated Varieties of Radicchio (Cichorium intybus L.): A Comparison between F1 Hybrids and Synthetics. Plants. 2019; 8(7):213. https://doi.org/10.3390/plants8070213
Chicago/Turabian StylePatella, Alice, Francesco Scariolo, Fabio Palumbo, and Gianni Barcaccia. 2019. "Genetic Structure of Cultivated Varieties of Radicchio (Cichorium intybus L.): A Comparison between F1 Hybrids and Synthetics" Plants 8, no. 7: 213. https://doi.org/10.3390/plants8070213
APA StylePatella, A., Scariolo, F., Palumbo, F., & Barcaccia, G. (2019). Genetic Structure of Cultivated Varieties of Radicchio (Cichorium intybus L.): A Comparison between F1 Hybrids and Synthetics. Plants, 8(7), 213. https://doi.org/10.3390/plants8070213