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Genome-Wide Identification and Characterization of JAZ Protein Family in Two Petunia Progenitors

1
Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
2
Key Laboratory of Landscape Architecture, Jiangsu Province, College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China
*
Author to whom correspondence should be addressed.
Plants 2019, 8(7), 203; https://doi.org/10.3390/plants8070203
Received: 28 May 2019 / Revised: 26 June 2019 / Accepted: 28 June 2019 / Published: 3 July 2019
(This article belongs to the Special Issue The Jasmonate Pathway: New Actors, Mechanisms and Impacts)
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Abstract

Jasmonate ZIM-domain (JAZ) family proteins are the key repressors in the jasmonate signaling pathway and play crucial roles in plant development, defenses, and responses to stresses. However, our knowledge about the JAZ protein family in petunia is limited. This research respectively identified 12 and 16 JAZ proteins in two Petunia progenitors, Petunia axillaris and Petunia inflata. Phylogenetic analysis showed that the 28 proteins could be divided into four groups (Groups A–D) and further classified into six subgroups (A1, A2, B1, B3, C, and D1); members in the same subgroup shared some similarities in motif composition and sequence structure. The Ka/Ks ratios of seven paralogous pairs were less than one, suggesting the petunia JAZ family might have principally undergone purifying selection. Quantitative real-time PCR (qRT-PCR) analysis revealed that PaJAZ genes presented differential expression patterns during the development of flower bud and anther in petunia, and the expression of PaJAZ5, 9, 12 genes was generally up-regulated after MeJA treatment. Subcellular localization assays demonstrated that proteins PaJAZ5, 9, 12 were localized in nucleus. Yeast two hybrid (Y2H) elucidated most PaJAZ proteins (PaJAZ1-7, 9, 12) might interact with transcription factor MYC2. This study provides insights for further investigation of functional analysis in petunia JAZ family proteins. View Full-Text
Keywords: JAZ; jasmonate; petunia; phylogenetic analysis; qRT-PCR; anther; MYC2 JAZ; jasmonate; petunia; phylogenetic analysis; qRT-PCR; anther; MYC2
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Tian, S.; Liu, S.; Wang, Y.; Wang, K.; Yin, C.; Yue, Y.; Hu, H. Genome-Wide Identification and Characterization of JAZ Protein Family in Two Petunia Progenitors. Plants 2019, 8, 203.

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