Next Article in Journal
S-Nitrosoglutathione Reductase—The Master Regulator of Protein S-Nitrosation in Plant NO Signaling
Next Article in Special Issue
Arabidopsis Natural Accessions Display Adaptations in Inflorescence Growth and Vascular Anatomy to Withstand High Salinity during Reproductive Growth
Previous Article in Journal
A Spotlight on Rad52 in Cyanidiophytina (Rhodophyta): A Relic in Algal Heritage
Previous Article in Special Issue
Sieve Plate Pores in the Phloem and the Unknowns of Their Formation
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Plants 2019, 8(2), 47; https://doi.org/10.3390/plants8020047

Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles

1
Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky Str., 2/31, 420111 Kazan, Russia
2
Laboratory of Extreme Biology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kremlyovskaya Str., 18, 420021 Kazan, Russia
3
Department of Plant Physiology and Biochemistry, Faculty of Biology, Saint Petersburg State University, Universiteskaya emb., 7/9, 199034 Saint Petersburg, Russia
4
Department of Genetics and Biotechnology, Faculty of Biology, Saint Petersburg State University, Universiteskaya emb., 7/9, 199034 Saint Petersburg, Russia
*
Author to whom correspondence should be addressed.
Received: 13 December 2018 / Revised: 13 February 2019 / Accepted: 14 February 2019 / Published: 19 February 2019
(This article belongs to the Special Issue Plant Phloem Development)
  |  
PDF [2390 KB, uploaded 19 February 2019]
  |  

Abstract

Phloem fibers are important elements of plant architecture and the target product of many fiber crops. A key stage in fiber development is intrusive elongation, the mechanisms of which are largely unknown. Integrated analysis of miRNA and mRNA expression profiles in intrusivelygrowing fibers obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known flax miRNA from 23 gene families and the potential targets of differentially expressed miRNAs. A comparison of the expression between phloem fibers at different developmental stages, and parenchyma and xylem tissues demonstrated that members of miR159, miR166, miR167, miR319, miR396 families were down-regulated in intrusively growing fibers. Some putative target genes of these miRNA families, such as those putatively encoding growth-regulating factors, an argonaute family protein, and a homeobox-leucine zipper family protein were up-regulated in elongating fibers. miR160, miR169, miR390, and miR394 showed increased expression. Changes in the expression levels of miRNAs and their target genes did not match expectations for the majority of predicted target genes. Taken together, poorly understood intrusive fiber elongation, the key process of phloem fiber development, was characterized from a miRNA-target point of view, giving new insights into its regulation. View Full-Text
Keywords: phloem fibers; intrusive growth; miRNA; transcriptome; laser microdissection; flax phloem fibers; intrusive growth; miRNA; transcriptome; laser microdissection; flax
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Gorshkov, O.; Chernova, T.; Mokshina, N.; Gogoleva, N.; Suslov, D.; Tkachenko, A.; Gorshkova, T. Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles. Plants 2019, 8, 47.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top