AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Expression Pattern of AtSIBP1
2.2. Identification of Arabidopsis sibp1 Mutant and AtSIBP1 Overexpression Lines
2.3. Tissue Expression Pattern of AtSIBP1 and Subcellular Localization of AtSIBP1-eGFP Fusion Protein
2.4. AtSIBP1 Acts as a Positive Regulator in Response to Salt Stress
2.5. Disruption or Overexpressing of AtSIBP1 Altered the Expression of Stress Induced Genes
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Generation of Transgenic Plants
4.3. Subcellular Localization Assay
4.4. Phenotype Analysis
4.5. GUS and DAB Staining
4.6. Analysis of Gene Expression
4.7. Sequence Analysis of AtSIBP1
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Wan, X.; Peng, L.; Xiong, J.; Li, X.; Wang, J.; Li, X.; Yang, Y. AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana. Plants 2019, 8, 573. https://doi.org/10.3390/plants8120573
Wan X, Peng L, Xiong J, Li X, Wang J, Li X, Yang Y. AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana. Plants. 2019; 8(12):573. https://doi.org/10.3390/plants8120573
Chicago/Turabian StyleWan, Xia, Lu Peng, Jie Xiong, Xiaoyi Li, Jianmei Wang, Xufeng Li, and Yi Yang. 2019. "AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana" Plants 8, no. 12: 573. https://doi.org/10.3390/plants8120573