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Article

Effect of Silicate Slag Application on Wheat Grown Under Two Nitrogen Rates

by
Brandon White
1,
Brenda S. Tubana
1,*,
Tapasya Babu
2,
Henry Mascagni
3,
Flavia Agostinho
1,
Lawrence E. Datnoff
4 and
Steve Harrison
1
1
School of Plant, Environmental, and Soil Sciences, Louisiana State University AgCenter, Baton Rouge, LA 70803, USA
2
Compass Minerals Innovation Center, Stilwell, KS 66085, USA
3
Northeast Research Station, Louisiana State University AgCenter, St. Joseph, LA 71366, USA
4
Department of Plant Pathology & Crop Physiology, Louisiana State University AgCenter, Baton Rouge, LA 70803, USA
*
Author to whom correspondence should be addressed.
Plants 2017, 6(4), 47; https://doi.org/10.3390/plants6040047
Submission received: 1 September 2017 / Revised: 23 September 2017 / Accepted: 9 October 2017 / Published: 11 October 2017
(This article belongs to the Special Issue Practical Use of Si to Influence Plant Production)
Browse Figures
Versions Notes

Abstract

:
Field studies were established on the alluvial floodplain soils in Louisiana, from 2013 to 2015, to evaluate the effect of silicate slag applications on productivity of wheat (Triticum aestivum), under sufficient and high nitrogen (N) application rates. Treatments were arranged in a randomized complete block design, with four replications consisting of twelve treatments: a factorial combination of two N (101 and 145 kg N ha−1) and five silicate slag rates (0, 1, 2, 4.5, and 9 Mg ha−1), and two control plots (with and without lime). Nitrogen had a greater impact on wheat productivity than silicate slag application. Wheat grain yield reached over 7000 kg ha−1 with applications of 145 kg N, and 9 Mg silicate slag per ha for soil having Si level <20 mg kg−1. Yield increases due to N or Si were attributed to the increase in number of spike m−2 and grain number spike−1. Silicate slag application effectively raised soil pH, and availability of several plant-essential nutrients, including plant-available N (nitrate, NO3−), demonstrating the benefits of slag application are beyond increasing plant-available Si. The benefits of silicate slag application were clearly observed in wheat supplied with high N, and on soil with low plant-available Si.

1. Introduction

Wheat is one of the most important crops worldwide, with global production exceeding that of all other crops [1]. More nourishment has been received from wheat for the world’s population than any other food grain [2]. The United States is one of the largest global producers of wheat, and ranked third in planted area during the first 10 years of the 2000s [3]. Wheat production area occupies about 60,000 ha in Louisiana with an estimated total value of $75 million in 2014 [4]. The high annual rainfall and high temperatures in Louisiana challenge wheat productivity through high pest and disease pressure, poor N utilization, and short grain fill periods [5]. Silicon (Si) is a beneficial plant nutrient that has been shown to improve yields in a variety of crops, especially members of Poaceae family [6]. Although wheat may accumulate close to 4% Si in plant tissue, research on this crop using Si lags far behind other crops, such as rice (Oryza sativa) and sugarcane (Saccharum officinarum) [7,8].
Silicon is prevalent in the soil, but primarily exists as silica (SiO2), which is not available for plant uptake. Silicon must be taken up by plant roots in the form of mono-silicic acid (H4SiO4), and the natural dissolution of SiO2 to H4SiO4 in the soil is slow [9]. Upon uptake, Si is deposited as amorphous silica (SiO2·nH2O) or opal phytoliths in cell lumens, cell walls, and intercellular spaces [9,10]. Once it is deposited, SiO2 is not redistributed within the plant [6]. The strengthening of these protective layers and the increase in overall structural integrity is one hypothesis for a number of benefits associated with Si uptake in plants. Silicon has been shown to increase resistance to multiple biotic and abiotic stresses, such as lodging, disease, and pest damage [7,11,12]. Positive responses of plant growth parameters to Si fertilization have been observed. Ma et al. (1989) reported increases in the number of panicles, spikelets per panicle, and decreases in the number of blank spikelets when Si was applied [13]. Increases in grain weight were also observed, as well as plant height and longer spikes in wheat [14,15]. These and other benefits of Si fertilization may contribute to yield increases.
Highly weathered, leached or organic soils, such as Histosols, are commonly deficient of available Si [16]. Soils planted to Si-accumulating crops can also diminish Si levels, furthering the potential responses to Si fertilization [17,18]. Silicate slags are common sources of Si, and are by-products from the steel manufacturing industry as well as from elemental phosphorus (P) production [19,20]. Silicon fertilization has become a common practice contributing to higher yields in crops such as rice and sugarcane [6]. The use of slags is widespread in Japan for degraded paddy soils in rice production [20]. Yoshida (1981) reported that yield increases of 10% are common in these and similar areas, and when leaf blast is severe, yield increases up to 30% were observed [21]. Using silicate slags, Korndorfer et al. (2001) reported yield increases in 19 out of 28 field experiments in rice production in the Everglades Agricultural Area in south Florida [22]. In a study conducted by Raid et al. (1992), sugar yield of cane applied with 6.7 Mg ha−1 were between 17.2 and 21.8% higher than the untreated cane for two successive cropping years [23]. Korndorfer et al. (2001) established 4.5 Mg ha−1 as an optimum rate for rice production, but responses in yield have been observed at up to 15 Mg ha−1 [22]. While higher rates are sometimes needed to reach greater yield increases, this is not always the case. Ma and Takahashi (2002) reported somewhat lower rates of 1.5 to 2 Mg ha−1 as common for many areas in Japan [19]. Like with other plant nutrients, fertilizer application rates can vary depending on several factors, including soil and crop type, as well as existing soil nutrient status.
Since N is the most limiting plant nutrient in non-leguminous crop production systems, and therefore, the most often applied among other nutrients, it is important to understand the relationship between Si and N. To prevent deficiencies, producers typically apply N in excess of the crop requirement. Excessive applications of N fertilizer can also result from not using effective N decision tools. Overuse of N fertilizers in agriculture is a non-point source of pollution to surface and groundwater systems [24]. However, excessive N application can also result in lodging, and increases in pest and disease damage [25,26,27], eventually reducing yield and income. These effects could be potentially minimized by the use of Si. Silicon has been reported to raise the optimum level of N in rice. Ho et al. (1980) reported that due to a synergistic effect, the application of Si has the potential to raise the optimum N rate use efficiency, thus enhancing productivity of existing lowland rice fields. Silicon has been reported to raise the optimum level of N in rice [28]. The interaction between Si and N fertilizer has been evaluated in crops like rice and sugarcane [12,29]. A study of the effects of N and Si nutrition on the susceptibility of sugarcane to the African sugarcane borer (Eldana saccharina) by Meyer and Keeping (2005) not only showed that Si reduced the susceptibility to the pest across multiple N rates, but that tissue N/Si ratios were more correlated with resistance to E. saccharina than N or Si alone [12]. A study by Wallace in 1989 found that as the N supply to a monocot species increased, the uptake of Si decreased [30].
While the benefits of Si in wheat have been documented, the combined effects of N and Si fertilization have not been evaluated, particularly in Louisiana. Therefore, this study was conducted to determine the impact of silicate slag application on grain yield of wheat under sufficient and high N application rates. The changes on N and Si uptake, biomass yield, soil pH, and plant-essential nutrient content of the soil, were also documented.

2. Results

The N, Si, and N × Si interaction effects on grain yield, N uptake, and Si uptake for all three site-years are reported in Table 1. Straw yield was only impacted by N, and only at Central Research Station in Ben Hur (BH) in 2014. A significant N × Si interaction effect on straw yield was observed at Northeast Research Station (NERS) in 2014. Nitrogen had a significant effect on grain yield at all three site-years, while a Si effect was only observed at BH in 2014 (p < 0.05). There was no significant interaction effect detected between N and Si on grain yield. Nitrogen had no recorded effect on straw N uptake except at BH in 2014, whereas for grain N uptake, both N and Si effects were significant at two of the three site-years. There was no noticeable effect of N × Si on Si uptake (straw, grain and total), except at NERS in 2014.
Trend analysis between grain yield and silicate slag rates for each level of N for each site-year is shown in Figure 1. This was also done to grain yield response of wheat with increasing silicate slag rate for each level of N. Higher grain yields were recorded for plots which received 145 kg N ha−1 at NERS in 2014 and BH in 2014. At BH in 2014, grain yield linearly increased with silicate slag rate, reaching >7000 kg ha−1, level with the application of 9 Mg ha−1 silicate slag, whereas at NERS in 2013, the application of silicate slag at 2 Mg ha−1 improved yield, but for plots with 101 kg N ha−1. At NERS in 2014, grain yield between 101 and 145 kg N ha−1 treatments were similar. The application of 2 Mg ha−1 silicate slag to plots which received 101 kg N ha−1 resulted in a significant increase in grain yield by >500 kg ha−1 (Figure 1). The results from the analysis of variance (ANOVA) on yield components showed that the impact of N, Si, and N × Si interaction was not consistently observed for all the three site-years (Table 2, Table 3 and Table 4). A general trend was that wheat applied with higher N rate tended to have higher number of spikes m−2. At NERS in 2013 and in 2014, the interaction effect of N and Si was significant (p = 0.08) if the level of confidence was set at 0.1. Here, the optimal silicate slag rate to attain the highest spike count was different for each N rate, i.e., 9 Mg ha−1 for 145 kg N ha−1, and between 2 to 4.5 Mg ha−1 for 101 kg N ha−1 (Table 2 and Table 3). Similarly, the effect of Si on grain count per spike was only observed in plots applied with 145 kg N ha−1. Overall, the higher number of spike m−2 and grain count per spike contributed to differences in grain yield observed in this trial. The average spike length and width did not respond to N and Si treatments. The number of grain per spike tended to be higher in wheat applied with 145 kg ha−1, than those with 101 kg N ha−1. The grain analysis showed that N consistently affected %N and %protein in grain with 145 kg N ha−1 having higher values than those applied with only 101 kg N ha−1. In addition, Si treatment impacted %N and %protein at NERS in 2014 and BH in 2014. Plots treated with silicate slag generally had higher N and protein content than plots without silicate slag. On the other hand, the lack of N and Si impact on Si content of grain was consistently observed across site-years.
Silicate slag application influenced soil pH and nutrient content more than the N application (Table 5, Table 6 and Table 7). Both soil pH and soil Si content based on 0.5 M acetic acid extraction procedure were increased with silicate slag application. Increasing silicate slag rate increased soil Si, with the highest increased by as much as 80 mg kg−1 (>150%) with the application rate of 9 Mg ha−1. Increases by at least 0.3 unit of pH were observed with a minimum of 2 Mg ha−1 application of silicate slag. With a 9 Mg ha−1 application rate of silicate slag, the highest increase in pH was 0.9 unit (Table 5 and Table 7). The application of silicate slag made a positive impact on the extracted amount of calcium (Ca), magnesium (Mg), S, manganese (Mn), Zn, and even copper (Cu) at NERS in 2014, based on Mehlich-3 extraction procedure. On the other hand, the amount of iron (Fe) extracted from the soil was reduced with an increasing rate of silicate slag (Table 7). Between ammonium (NH4+) and NO3−, silicate slag rate increased the amount of NO3− extracted from the soil in all three site-years. Nitrogen treatment had an effect on the NH4+ and NO3− content, being higher in plots which received 145 kg ha−1 than those with 101 kg N ha−1 application, but only at NERS in 2014 and BH in 2014.

3. Discussion

Results on grain yield from site-years at NERS in 2013 and BH in 2014 demonstrated that N had a higher impact on wheat grain yield than Si. This finding is not unexpected, since N is the most limiting nutrient in crop production, and it is common to obtain observable grain yield responses to N application rather than Si. Heavy rainfall was recorded around the time of N application, which could have resulted in loss of the N applied from the soil profile through leaching and a denitrification process. At NERS in 2014, the total amount of rain received was ~500 mm for the months of January and February. At NERS in 2013, an average of 180 mm rainfall was received in March and April following 100 mm rain received in February. This could partly explain the greater grain yield response to N observed at BH in 2014 (Figure 1). Cereal crops and sugarcane recovery of applied N fertilizers is low, ranging from only 20% to 40%, with ~65% presumably lost from the crop–soil systems [31,32,33,34]. In the present study, the NO3− content of the soil was consistently lower than NH4+ across the site-years; this is associated with the mobility of NO3− in the soil, being 5 to 10 times faster than NH4+ and other N forms, such as amino acids [35].
The high rainfall and subsequent leaching could have also led to the loss of applied Si from the plant root zones. This might also explain the lack of wheat grain yield response to Si application, even though the soil Si level was considered to be low (36 mg kg−1). The severe and frequent soil erosion and sediment transportation in areas owing to high rainfall and coarse texture of soil might also have led to desilication, and thus, a relatively low soil Si [36]. Among the site-years, BH in 2014 had the lowest initial soil Si level (17 mg kg−1); this soil Si level is below the initial critical soil Si level established for soils of Louisiana [37]. The effect of Si on yield was significant, with higher yields observed at the highest silicate slag rate at 9 Mg ha−1 for both N rates (Figure 1). It is notable that grain yield was the highest (~7000 kg ha−1) from plots which received 145 kg N ha−1 and 9 Mg ha−1 silicate slag, suggesting the important role of Si in minimizing the yield decline factors, like lodging and pest and disease damage that may have been caused by excessive N application [29]. Korndorfer et al. (2001) reported yield increases in rice with Si fertilization in several different field experiments [22]. Similar yield increases have also been reported for rice production in Japan [21] and in wheat [15,38]. While 9 Mg ha−1 was the highest yielding silicate slag rate for both N rates at BH in 2014, it is important to note that this high rate may limit the economic return and feasibility of application of silicate slag. Slag materials are by-products and relatively inexpensive compared to other common fertilizers, but transportation costs are expensive for fields that are quite far from slag manufacturers (or suppliers). Also, the amount of yield increase should be weighed out carefully to justify the cost of applying high rates of silicate slag. In addition, soils which have high pH may encounter nutrient-related problems as silicate slag is a potent liming agent [38], and at this rate, might cause precipitation of micronutrients, thus causing a deficiency in crops.
The pH, 0.5 M acetic acid extractable Si, soil NO3− and NH4+, and Mehlich-3-extractable nutrients of soil sampled at harvest, are summarized in Table 5, Table 6 and Table 7 for each site-year. Silicate slag has a high liming potential [38], thus effective at increasing soil pH. As a by-product, silicate slag contains other elements, such as Ca, Mg, Mn, Fe, and S. The soil analysis showed that all of these nutrients, except for Fe, increased with the application of silicate slag. Silicon rates significantly increased the amount of Ca, Mg, Mn, and S in the soil, but this was expected because the silicate slag material used in these trials contains these elements. While the significant increases in S at NERS in 2013 and BH in 2014 can be attributed to the direct result of addition of silicate slag due to the presence of S in the material, the increase in pH may have also influenced the increase in soil S extracted. The soil pH at NERS in 2013 and BH in 2014 was increased by 0.9 units of pH with silicate slag application, whereas the soil pH of NERS in 2014 reached a maximum of only 5.5 (acidic). The increase in soil pH can considerably reduce the adsorption of sulfate ions (SO42−) because adsorption of sulfate occurs on positively charged sites in a soil matrix that are pH dependent, and becomes negligible at pH values greater than 6 [39,40]. Similarly, the increase in addition of silicate slag could have resulted in an increase of the SiO32− ion competition for the anion sorption sites in soil. The reduction in pH dependent anion sorption sites in soils of NERS in 2013 and BH in 2014 due to the rise in pH up to ~7, combined with an increase in SiO32− ion competition for these sites in soil, could have drastically reduced the sorption of SO42− at these sites. Management practices, such as liming and phosphate additions, are known to release some of the adsorbed SO42− [41]. Therefore, increase in plant available S in soil could not only have been a direct but also an indirect result from the addition of silicate slag as well. On the other hand, the reduction in Mehlich-3 extractable Fe content of the soil at NERS in 2014 may likely be associated with Fe precipitation due to the increased soil pH. While Zn is not a component of the silicate slag material, its availability increased as Si rates increased for all three site-years (p < 0.05). These results contradict what was expected, as Zn availability decreases with increasing soil pH [42]. However, Zn availability still increased in these soils despite the rise in soil pH observed as silicate slag rates increased. These results disagree with those by Saleh et al. (2013) and Cunha et al. (2008), where Si applications lead to decreases in extractable Zn [43,44]. This finding could simply be due to pH, since in this study, it was never above 7. Although soil pH governs the speciation of Zn in solution, Zn2+ (plant available form) predominates at pH values below 7.7, and ZnOH+ is the main species at pH above 7.7, and the neutral species Zn(OH)2 is dominant only at pH above 9 [45]. The increased availability of Zn in soil could also be due to the increase in concentration of Ca and Mg in soil, consequent with the addition of slag. Zhu and Alva (1993) studied the relative effect of Ca, Mg, and K on the adsorption of Zn and Cu [46]. Adsorption of both Cu and Zn decreased with an increase in concentrations of either Ca or Mg. This reduction in adsorption was attributed to increased competition for exchange sites with increased ionic strength. They also reported that this inhibitory effect of added cations on metal adsorption was greater for Zn than Cu.
The percent organic matter of BH in 2014 was lower among the three site-years studied with NERS in 2013, NERS in 2014, and BH in 2014 having 2.3%, 2.1%, and 1.5% organic matter, respectively. It seems that in the soils of NERS 2013 and 2014, the solution concentration of Al was reduced by the sorptive properties of organic matter or the formation of Al ion complexes by organic matter (organic acids) [47,48,49,50,51,52,53,54]. Also, the initial concentration of Ca, Mg, and K, at NERS in 2013 and 2014, were greater than BH 2014, with only 1833, 481, and 146 mg kg−1 of Ca, Mg, and K, respectively. Zołotajkin et al. (2011) documented the presence of cations which compete with Al for exchange sites as an additional factor that might govern the concentration of exchangeable Al in the soil [55]. Similarly, it might be the presence of cations which compete with Al for exchange sites that resulted in lower concentration of exchangeable Al at NERS in 2013 and 2014. With comparatively lower concentration of these cations at BH in 2014 soil, it is likely that Al occupied the exchange sites over these basic cations. These basic cations, which are then present in soil solution, are easily lost from the surface soil due to various processes like leaching, runoff, and plant uptake over the period of crop growth. Whereas in the case of NERS in 2013 and 2014, the relatively higher concentration of these basic cations that compete for exchange sites with Al could have kept them in the surface soil for longer duration and at a higher concentration. However, this conclusion needs to be confirmed by further research.
With respect to agronomic responses, the application of N resulted in more consistent and significant changes in grain yield, yield components, and grain composition than silicate slag application. Increases in grain yield in plots which received higher N rate (145 kg N ha−1) were associated with higher number of spikes m−2 combined with higher numbers of grain per spike. The higher supply of N also improved %N and %protein content of grain. Previous studies showed similar effects on wheat yield components and quality [56,57]. However, there was no associated improvement on average spike width and length, or 1000 g weight with increased N rate. In fact, there was a tendency for these components to be lower in plots with 145 kg N ha−1 than plots with 101 kg N ha−1. This is consistent with the report of Barbottin et al. (2005) on the higher N remobilization observed in wheat under low N supply compared to high N supply [58]. On the impact of Si, it was only at BH in 2014 where the application of silicate slag application made a significant impact on grain yield (Figure 1), and this increase can be attributed to an increase in number of spikes m−2 (Table 4). The improvement in grain yield can also be tied up with the increase in soil NO3− content, along with other essential nutrients, when silicate slag was applied. Between N and silicate slag, this study demonstrated the stronger influence of silicate slag application on maintaining an elevated level of NO3− in the soil, as opposed to N application, and also, improvement in the levels of some plant-essential nutrients and soil pH.

4. Materials and Methods

This study was conducted from 2012 to 2014 with a total of three site-years. The first site-year was established in 2012 at St. Joseph (Latitude 31°56′42.6″ N; Longitude 91°13′34″ W), Louisiana on a Commerce silt loam soil (fine-silty, mixed, superactive, nonacid, thermic Fluvaquentic Endoaquepts). In 2013, this study was established at two locations: (1) NERS, St. Joseph, Louisiana (Latitude 31°56′41.0″ N; Longitude 91°13′25.8″ W) on a field with Sharkey–Tunica–Newellton complex (very fine, smectitic, thermic Chromic Epiaquerts) and Commerce silt loam soil types and (2) BH (Latitude 30°21′40.4″ N; Longitude 91°10′01.9″ W) near Louisiana State University campus in Baton Rouge on a Cancienne silt loam soil (fine-silty, mixed, superactive, nonacid, hyperthermic Fluvaquentic Epiaquepts). All of these soils are found on the alluvial floodplain along the Mississippi River. Figure 2 summarizes the cumulative growing degree days and monthly rainfall distribution during the cropping season for each site-year. Nitrogen fertilizer was applied in February, and among the site-years, Ben Hur in 2014 received the highest amount of rain. In addition, the rate of accumulation of positive growing degree days was slower in this site-year compared to NERS 2013 and 2014.
All three site-years were established using a dryland, conventional tillage system. Table 8 provides information on the locations, site-year ID and major field activities for the three site-years. Initial soil data for all three site-years is summarized in Table 9. The treatment structure was a two-way factorial (two N rates x five silicate slag rates) in a randomized complete block design with four replications. Each replication consisted of two checks (no N and Si applied), without lime and with lime at 4.5 Mg ha−1. The lime treatment was incorporated into the treatment structure to differentiate treatment effects from a pH effect, since slags are a potent liming agent. Two N rates of 101 and 145 kg ha−1 were used for this study using urea (46% N) as the N source. The 101 kg N ha−1 rate is the standard rate for wheat production in Louisiana, and the 145 kg N ha−1 rate is considered a high application rate, a rate sometimes used by farmers in the state. For each N rate, there were five silicate slag (Plant Tuff®, 12% Si, Dearborn, MI, USA) rates of 0, 1, 2, 4.5, and 9 Mg ha−1, which were equivalent to 0, 120, 240, 540, and 1080 kg Si ha−1, respectively. Phosphorus and potassium (K) fertilizer were applied to fields when necessary, according to the test results and recommendations of the LSU AgCenter Soil Testing and Plant Analysis Laboratory, to ensure neither nutrient was limiting in the soil. Silicate slag is the source of Si used for this study, which is a byproduct from steel industries containing 12% Si. Other known components of the slag material used in this study include Ca (23%), Mg (7%), and S (0.5%), among others. Both slag and lime treatments were broadcast applied by hand to individual plots in November of each year. Treatments were then incorporated into the soil to a depth of about 7.5 cm. Winter wheat variety Terral 8525 was drill seeded at the rate of 101 kg ha−1 for NERS in 2013 and 2014, and 113 kg ha−1 for BH in 2014 within, a week of slag and lime applications. The two N treatments were applied around Feekes growth stage (GS) 4 [59]. Urea (46%N) was broadcast applied by hand to the corresponding plot assignment. Recommended weed management practices from the LSU AgCenter were followed.
Soil samples were taken at harvest, oven-dried (Despatch LBB series; model number LBB2-18-1) at 55 °C for about 4–5 days, and then ground using a Humboldt soil grinder, and passed through a 2 mm sieve for later analysis. Extractable Si was determined by extracting soil samples with 0.5 M acetic acid (10 mL extractant and 1 g soil shaken for 1 h), followed by a modified molybdenum blue colorimetry (MBC) procedure, as outlined by Korndorfer et al. (2001) [22]. Soil inorganic N was determined by KCl extraction, followed by spectrophotometric measurement using an automated flow injection system (Lachat QuickChem 8500 series 2), similar to the method described by Keeney and Nelson (1982) [60]. Soil pH was also determined in a 1:1 ratio of soil:deionized (DI) water suspension using a pH meter. A wide range of extractable nutrients in the soil were determined by Mehlich-3 Procedure [61]. Two (2) grams of soil was extracted with 20 mL of Mehlich-3 solution (dilute acid–fluoride–EDTA solution, pH 2.5), and the extract was then analyzed using inductively coupled plasma (ICP)–optical emission spectroscopy (OEM), for several essential nutrients as well as some heavy metals (Spectro Ciros CCD ICP analyzer, SPECTRO Analytical Instruments, Kleve, Germany).
All plots were harvested using plot combine harvesters, and grain subsamples taken simultaneously were measured for moisture content, test weight, and total weight. Grain moisture content was adjusted to 12%, and yield was calculated in kg per ha−1.
The results on the analysis of variance (ANOVA) using PROC MIXED in SAS 9.3 on the effect of site-year, N, and Si, showed significant effect of site-year and the three-way interaction (site-year × N × Si) for most of the measured plant variables (not shown). Thus, the ANOVA was performed for each site-year to determine the effects of N, Si, and N × Si interactions on all measured parameters (SAS, 2012). The fixed effects were N, Si, and their interaction, while replication was assigned as a random effect. Treatments 1 (control) and 2 (check lime) were deleted, and the program was run as a complete factorial, in order to determine significant differences in Si treatments at each N rate. Trend analysis between grain yield and silica slag rates for each N rate of each site-year was conducted. For any significant effect at level <0.10, mean separation using LSD followed with identify treatment differences. Means of all measured parameters for all N × Si combinations were reported and compared, to identify the combination(s) which attained the optimal agronomic responses.

5. Conclusions

Nitrogen more consistently impacted wheat grain yield in comparison to silicate slag application across site-years. While increasing numerical trends of grain yield were observed for all Si treatment levels when compared to control treatments, significant increases were observed for 2 and 9 Mg ha−1 silicate slag rates for BH in 2014 only. NERS in 2013 initial soil Si levels were higher than both sites in 2014, and higher soil Si levels were recorded for soil samples collected at harvest. Nitrogen and Si both had effects on the availability of certain essential nutrients and heavy metals. The applications of silicate slag increased the 0.5 M acetic acid extractable Si and soil pH. Silicate slag applications showed effects on extractable Ca, Mg, S, Zn, NO3−, and Si in all three site-years. Silicate slag is a byproduct and contains some of the elements that were analyzed in the soil, and may partially explain some of the increases in availability observed. Although the other nutrients present in the slag were not found limiting in the soil, it is possible that the increased availability of other nutrients, whether direct effects from slag composition or indirect effects from interactions and pH, could have contributed to increases in grain yields. The application of silicate slag can influence the nitrification rate and NO3− availability in soils from the N fertilizers by way of influencing soil pH. Because of the effectiveness of silicate slag at increasing soil Si and soil pH, it could prove even more beneficial if used as a lime treatment in place of traditional CaCO3. The inconsistencies observed in responses to Si treatments could be due to varying physicochemical properties of soils belonging to different series, generally identified as three major groups in a calibration study conducted for establishing fertilizer guidelines. More research will need to be conducted to further calibrate application rates for silicate slag for use in wheat production in Louisiana.

Acknowledgments

Published with the approval of the Director of the Louisiana Agricultural Experiment Station as publication 2017-306-31424. This research was funded in part by USDA-NIFA and Edward C. Levy Co.

Author Contributions

B.W., L.E.D. and B.S.T. conceived and designed the experiments; B.W., B.S.T., S.H., T.B. and H.M. performed the experiments; B.W., T.B. and B.S.T. analyzed the data and wrote the manuscript; L.D., F.A. and B.S.T. prepared the manuscript in accordance to Plant’s format.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Briggle, L.W.; Curtis, B.C. Wheat worldwide. In Wheat and Wheat Improvement; Heyne, E.G., Ed.; American Society of Agronomy, Crop Science Society of America, Soil Science Society of America: Madison, WI, USA, 1987; pp. 1–32. [Google Scholar]
  2. Reitz, L.P. World distribution and importance of wheat. In Wheat and Wheat Improvement; Quisenberry, K.S., Reitz, L.P., Eds.; American Society of Agronomy: Madison, WI, USA, 1967; pp. 1–18. [Google Scholar]
  3. United States Department of Agriculture-Economic Research Service. Wheat/Background. 2014. Available online: http://www.ers.usda.gov/topics/crops/wheat/background.aspx (accessed on 18 February 2015).
  4. Westra, J.; Niu, H. Louisiana Summary of Agriculture and Natural Resources; Publication No. 2393; LSU AgCenter: Baton Rouge, LA, USA, 2014. [Google Scholar]
  5. Mascagni, H.J., Jr.; Harrison, S.A.; Russin, J.S.; Desta, H.M.; Colyer, P.D.; Habetz, R.J.; Hallmark, W.B.; Moore, S.H.; Rabb, J.L.; Hutchinson, R.L.; et al. Nitrogen and fungicide effects on winter wheat produced in the Louisiana Gulf Coast region. J. Plant Nutr. 1997, 20, 1375–1390. [Google Scholar] [CrossRef]
  6. Epstein, E. Silicon. Ann. Rev. Plant Physiol. Plant Mol. Biol. 1999, 50, 641–664. [Google Scholar] [CrossRef] [PubMed]
  7. Ma, J.F.; Miyake, Y.; Takahashi, E. Silicon as a beneficial element for crop plants. In Silicon in Agriculture; Datnoff, L.E., Snyder, G.H., Korndörfer, G.H., Eds.; Elsevier: Amsterdam, The Netherlands, 2001; pp. 17–39. [Google Scholar]
  8. Rafi, M.M.; Epstein, E. Silicon absorption by wheat (Triticum. aestivum L.). Plant Soil 1999, 211, 223–230. [Google Scholar] [CrossRef]
  9. Raven, J.A. The transport and function of silicon in plants. Biol. Rev. 1983, 58, 179–207. [Google Scholar] [CrossRef]
  10. Marschner, H.; Oberle, H.; Cakmak, I.; Romheld, V. Growth enhancement by silicon in cucumber plants (Cucumis. sativus) depends on imbalance on phosphorus and zinc supply. Plant Soil 1990, 124, 211–219. [Google Scholar] [CrossRef]
  11. Fallah, A. Silicon effect on lodging parameters of rice plants under hydroponic culture. Int. J. AgriSci. 2012, 2, 630–634. [Google Scholar]
  12. Meyer, J.H.; Keeping, M.G. The impact of nitrogen and silicon nutrition on the resistance of sugarcane varieties to Eldana saccharina (Lepidoptera: Pyralidae). Proc. S. Afr. Sugar Technol. Assoc. 2005, 79, 363–367. [Google Scholar]
  13. Ma, J.F.; Nishimura, K.; Takahashi, E. Effect of silicon on the growth of rice plant at different growth stages. Soil Sci. Plant Nutr. 1989, 35, 347–356. [Google Scholar] [CrossRef]
  14. Balasta, M.L.F.C.; Perez, C.M.; Juliano, B.O.; Villareal, C.P.; Lott, J.N.A.; Roxas, D.B. Effects of silica level on some properties of Oryza sativa straw and hull. Can. J. Bot. 1989, 67, 2356–2363. [Google Scholar] [CrossRef]
  15. Abro, S.A.; Qureshi, R.; Soomro, F.M.; Mirbahar, A.A.; Jakhar, G.S. Effects of silicon levels on growth and yield of wheat in silty loam soil. Pak. J. Bot. 2009, 41, 1385–1390. [Google Scholar]
  16. Foy, C.D. Soil chemical factors limiting plant root growth. Adv. Soil Sci. 1992, 19, 97–149. [Google Scholar]
  17. Meyer, J.H.; Keeping, M.G. Past, present and future research of the role of silicon for sugarcane in southern Africa. In Silicon in Agriculture; Datnoff, L.E., Snyder, G.H., Korndörfer, G.H., Eds.; Elsevier: Amsterdam, The Netherlands, 2001; pp. 257–275. [Google Scholar]
  18. Savant, N.K.; Datnoff, L.E.; Snyder, G.H. Depletion of plant-available silicon in soils: A possible cause of declining rice yields. Commun. Soil Sci. Plant Anal. 1997, 28, 1245–1252. [Google Scholar] [CrossRef]
  19. Ma, J.F.; Takahashi, E. Soil, Fertilizer, and Plant Silicon Research in Japan; Elsevier: Amsterdam, The Netherlands, 2002. [Google Scholar]
  20. Alvarez, J.; Snyder, G.H.; Anderson, D.L.; Jones, D.B. Economics of calcium silicate slag application in a rice-sugarcane rotation in the Everglades. Agric. Syst. 1988, 28, 179–188. [Google Scholar] [CrossRef]
  21. Yoshida, S. Fundamentals of Rice Crop Science; International Rice Research Institute: Los Banos, Laguna, Philippines, 1981. [Google Scholar]
  22. Korndörfer, G.H.; Snyder, G.H.; Ulloa, M.; Powell, G.; Datnoff, L.E. Calibration of soil and plant silicon analysis for rice production. J. Plant Nutr. 2001, 24, 1071–1084. [Google Scholar] [CrossRef]
  23. Raid, R.N.; Anderson, D.L.; Ulloa, M.F. Influence of cultivar and amendment of soil with calcium silicate slag on foliar disease development and yield of sugarcane. Crop Prot. 1992, 11, 84–88. [Google Scholar] [CrossRef]
  24. Carpenter, S.R.; Caraco, N.F.; Correll, D.L.; Smith, V.H. Non-point pollution of surface waters with phosphorus and nitrogen. Ecol. Appl. 1998, 8, 559–568. [Google Scholar] [CrossRef]
  25. Berry, P.M.; Griffin, J.M.; Sylvester-Bradley, R.; Scott, R.K.; Spink, J.H.; Baker, C.J.; Clare, R.W. Controlling plant form through husbandry to minimize lodging in wheat. Field Crops Res. 2000, 67, 59–81. [Google Scholar] [CrossRef]
  26. Miller, B.S.; Robinson, R.J.; Johnson, J.A.; Jones, E.T.; Ponnaiya, B.W.X. Studies on the relation between silica in wheat plants and resistance to Hessian fly attack. J. Econ. Entomol. 1960, 53, 995–999. [Google Scholar] [CrossRef]
  27. Slaton, N.A.; Cartwright, R.D.; Meng, J.; Gbur, E.E.; Norman, R.J. Sheath blight severity and rice yield as affected by nitrogen fertilizer rate, application method, and fungicide. Agron. J. 2003, 95, 1489–1496. [Google Scholar] [CrossRef]
  28. Ho, D.Y.; Zhang, H.L.; Zhang, X.P. The silicon supplying ability of some important paddy soils of South China. In Proceedings of the Symposium on Paddy Soil, Naying, China, 19–24 October 1980; pp. 19–24. [Google Scholar]
  29. Mauad, M.; Crusciol, C.A.C.; Filho, H.G.; Correa, J.C. Nitrogen and silicon fertilization of upland rice. Scientia Agricola 2003, 60, 761–765. [Google Scholar] [CrossRef]
  30. Wallace, A. Relationships among nitrogen, silicon, and heavy metal uptake by plants. Soil Sci. 1989, 147, 457–460. [Google Scholar] [CrossRef]
  31. Kingston, G.; Anink, M.C.; Allen, D. Acquisition of nitrogen by ratoon crops of sugarcane as influenced by waterlogging and split applications. Proc. Conf. Aust. Soc. Sugar Cane Technol. 2008, 30, 202–211. [Google Scholar]
  32. Franco, H.; Otto, R.; Faroni, C.; Vitti, A.; Oliveira, E.; Trivelin, P.O. Nitrogen in sugarcane derived from fertiliser under Brazilian field conditions. Field Crops Res. 2011, 121, 29–41. [Google Scholar] [CrossRef]
  33. Meyer, J.; Schmann, A.; Wood, R.; Nixon, D.; Van Den Berg, M. Recent advances to nitrogen use efficiency of sugarcane in the South African sugar industry. Proc. Int. Soc. Sugar Cane Technol. 2007, 26, 238–246. [Google Scholar]
  34. Raun, W.R.; Johnson, G.V. Improving nitrogen use efficiency for cereal production. Agron. J. 1999, 91, 357–363. [Google Scholar] [CrossRef]
  35. Owen, A.G.; Jones, D.J. Competition for amino acids between wheat roots and rhizosphere microorganisms and the role of amino acids in plant N acquisition. Soil Biol. Biochem. 2001, 33, 651–657. [Google Scholar] [CrossRef]
  36. White, A.F. Chemical weathering rates of silicate minerals in soils. Rev. Min. Geochem. 1995, 31, 407–461. [Google Scholar]
  37. Tubana, B.S.; Martins, M.; Dalen, M.; Kanke, Y.; Shahrtash, M.; White, B.; Babu, T.; Mascagni, H. Impact of silicate slag fertilization on wheat yield and grain protein content. In Proceedings of the ASA-CSSA-SSSA International Annual Meetings, Phoenix, AZ, USA, 6–9 November 2016. [Google Scholar]
  38. Chand, S.; Paul, B.; Kumar, M. An overview of use of Linz-Donawitz (LD) steel slag in agriculture. Curr. World Environ. 2015, 10, 975–984. [Google Scholar] [CrossRef]
  39. Tabatabai, M.A. Sulfur. In Methods of Soil Analysis, Part II, 2nd ed.; Chemical and Microbiological Properties; Page, A.L., Miller, R.H., Keeney, D.R., Eds.; American Society of Agronomy: Madison, WI, USA, 1982; pp. 501–538. [Google Scholar]
  40. Tabatabai, M.A. Physicochemical Fate of Sulfate in Soils. J. Air Waste Manag. Assoc. 1987, 37, 34–38. [Google Scholar] [CrossRef]
  41. Chao, M.L.; Thomas, G.W. A suggested mechanism for sulphate adsorption by soils. Soil Sci. Soc. Am. Proc. 1963, 27, 281–283. [Google Scholar]
  42. Havlin, J.L.; Beaton, J.D.; Tisdale, S.L.; Nelson, W.L. Soil Fertility and Fertilizers: An Introduction to Nutrient Management; Pearson Prentice Hall: Upper Saddle River, NJ, USA, 2005. [Google Scholar]
  43. Cunha, K.; Nascimento, C.; Silva, A.J. Silicon alleviates the toxicity of cadmium and zinc for maize (Zea mays L.) grown on a contaminated soil. J. Plant Nutr. Soil Sci. 2008, 171, 849–853. [Google Scholar] [CrossRef]
  44. Saleh, J.; Najafi, N.; Oustan, S.; Aliasgharzad, N.; Ghassemi-Golezani, K. Effects of silicon, salinity and water logging on the extractable Zn, Cu, K and Na in a sandy loam soil. Int. J. Agric. Res. Rev. 2013, 3, 56–64. [Google Scholar]
  45. Alloway, B.J. Zinc in Soils and Crop Nutrition, 2nd ed.; International Zinc Association and International Fertilizer Association: Brussels, Belgium; Paris, France, 2008. [Google Scholar]
  46. Zhu, B.; Alva, A.K. Differential adsorption of trace metals by soils as influenced by exchangeable cations and ionic strength. Soil Sci. 1993, 155, 61–66. [Google Scholar] [CrossRef]
  47. Kotowski, M.; Pawłowski, L.; Zhu, X. Aluminum in the Environment; Wydawnictwo Politechniki Lubelskiej: Lublin, Poland, 1995. (In Polish) [Google Scholar]
  48. Thomas, G.W. The relationship between organic matter content and exchangeable aluminum in acid soils. Soil Sci. Soc. Am. J. 1976, 39, 591. [Google Scholar] [CrossRef]
  49. Bloom, P.R.; McBridge, M.B.; Weaver, R.M. Aluminum organic matter in acid soil: Buffering and solution aluminum activity. Soil Sci. Soc. Am. J. 1979, 43, 488–493. [Google Scholar] [CrossRef]
  50. Lundstrom, U.S. The role of organic acids in the soil solution chemistry of a podzolized soil. J. Soil Sci. 1993, 44, 121–133. [Google Scholar] [CrossRef]
  51. Wesselink, L.; Mulder, J. Modelling Al-solubility controls in an acid forest soil, Solling, Germany. Ecol. Model. 1995, 83, 109–117. [Google Scholar] [CrossRef]
  52. Wit, H.A.; Kotowski, M.; Mulder, J. Modelling aluminium and organic matter solubility in the forest floor using WHAM. Soil Sci. Soc. Am. J. 1999, 63, 1141–1148. [Google Scholar] [CrossRef]
  53. Berggren, D.; Muller, J. The role of organic master in controlling aluminum solubility in acidic mineral horizons. Geochim. Cosmochim. Acta 1995, 59, 4167–4180. [Google Scholar] [CrossRef]
  54. Mulder, J.; Wit, H.A.; Boonen, H.W.J.; Bakken, L.R. Increased levels of aluminum in forest soils: Effects on the stores of soil organic carbon. Water Air Soil Pollut. 2001, 130, 989–994. [Google Scholar] [CrossRef]
  55. Zołotajkin, M.; Ciba, J.; Kluczka, J.; Skwira, M.; Smoliński, A. Exchangeable and Bioavailable Aluminum in the Mountain Forest Soil of Barania Góra Range (Silesian Beskids, Poland). Water Air Soil Pollut. 2011, 216, 571–580. [Google Scholar] [CrossRef] [PubMed]
  56. Ali, L.Q.; Qamar, M.; Ali, M. Effect of different doses of nitrogen fertilizer on the yield of wheat. Int. J. Agric. Biol. 2003, 5, 438–439. [Google Scholar]
  57. Nakano, H.; Morita, S.; Kusuda, O. Effect of nitrogen application rate and timing on grain yield and protein content of the bread wheat cultivar ‘Minaminokaori’ in Southwestern Japan. Plant Prod. Sci. 2008, 11, 151–157. [Google Scholar] [CrossRef]
  58. Barbottin, A.; Lecomte, C.; Bouchard, C.; Jeuffroy, M.H. Nitrogen remobilization during grain filling in wheat: Genotypic and environmental effects. Crop Sci. 2005, 45, 1141–1150. [Google Scholar] [CrossRef]
  59. Large, E.C. Growth stages in cereals: Illustration of Feekes scale. Plant Pathol. 1954, 3, 128–129. [Google Scholar] [CrossRef]
  60. Keeney, D.R.; Nelson, D.W. Nitrogen-Inorganic forms. Methods Soil Anal. 1982, 9, 643–698. [Google Scholar]
  61. Mehlich, A. Mehlich 3 soil test extractant: A modification of Mehlich 2 extractant. Commun. Soil Sci. Plant Anal. 1984, 15, 1409–1416. [Google Scholar] [CrossRef]
Plants 06 00047 g001 550
Figure 1. Grain yield of wheat applied with varying rates of silicate slag under sufficient and high N application rates NERS 2013, NERS 2014, and BH 2014. NS denotes no significant effect on yield; P values reported are for the regression model between yield and silicate slag for each N rate.
Figure 1. Grain yield of wheat applied with varying rates of silicate slag under sufficient and high N application rates NERS 2013, NERS 2014, and BH 2014. NS denotes no significant effect on yield; P values reported are for the regression model between yield and silicate slag for each N rate.
Plants 06 00047 g001
Plants 06 00047 g002 550
Figure 2. Cumulative growing degree days and monthly rainfall distribution from the beginning (November) and the end (June) of cropping season for each site-year.
Figure 2. Cumulative growing degree days and monthly rainfall distribution from the beginning (November) and the end (June) of cropping season for each site-year.
Plants 06 00047 g002
Table
Table 1. Analysis of variance on yield, nitrogen, and silicon uptake for straw and grain for each site-year.
Table 1. Analysis of variance on yield, nitrogen, and silicon uptake for straw and grain for each site-year.
Site-Year FactorsYieldNitrogen UptakeSilicon Uptake
StrawGrainStrawGrainStrawGrainTotal
NERS 2013
N0.9620.0400.3110.1040.3360.1710.126
Si0.1500.4170.6260.0020.2700.4660.256
N × Si0.0870.6030.8410.0710.3820.7540.389
NERS 2014
N0.2230.0020.158<0.0010.3210.2410.245
Si0.5400.7830.3030.3550.1980.1270.370
N × Si0.0610.8140.0050.966<0.0010.0420.003
BH 2014
N0.002<0.0010.003<0.0010.3510.9280.151
Si0.6190.0140.4590.0030.3520.5680.303
N × Si0.2350.5620.4800.7440.4940.0680.227
Notes: N—nitrogen, Si—silicon; NERS—Northeast Research Station in St. Joseph, Louisiana, USA; BH—Central Research Station in Ben Hur Farm near LSU Campus in Baton Rouge, Louisiana, USA.
Table
Table 2. Mean and analysis of variance of wheat yield components, grain N and Si content, and % protein for site-year NERS 2013.
Table 2. Mean and analysis of variance of wheat yield components, grain N and Si content, and % protein for site-year NERS 2013.
N kg ha−1Slag Mg ha−1YieldSpikeGrain per Spike1000-Grain gNSiProtein
kg ha−1Count per m2Weight gLength cm%
10104896606 bc1.297.2424.2 bc33.01.770.06010.3
15265600 bc1.437.4226.0 bc32.71.750.06210.2
25504634 ab1.437.6426.3 bc33.01.780.07010.4
4.55370614 bc1.407.3526.2 bc32.91.780.07710.4
95152587 bc1.287.4225.7 bc32.61.800.07210.4
mean5237 A6081.367.4125.732.8 A1.77 B0.06810.3 B
14504879570 bc1.357.4428.7 ab31.61.860.08310.8
14761648 ab1.367.3124.0 c30.21.860.06210.9
24861648 ab1.357.4725.1 bc30.21.870.07710.9
4.55209530 c1.367.3932.1 a31.11.850.06810.8
94991704 a1.277.1424.3 c29.81.950.04811.4
mean4940 B6201.347.3726.930.6 B1.88 A0.06811.0 A
Analysis of variance
Np-value0.0400.8170.4390.6160.267<0.001<0.0010.888<0.001
Sip-value0.4190.3260.0970.2820.0610.5670.4510.4900.485
N × Sip-value0.6040.0350.6200.5160.0560.7300.8590.1570.856
Notes: N—nitrogen; slag—silicate slag rate. Actual p-values are reported. Values with the same uppercase (N treatment) and lowercase (across N and Si combinations) letters are not significantly different according to least square difference LSD at 0.05 level of confidence.
Table
Table 3. Mean and analysis of variance of wheat yield components, grain N and Si content, and % protein for site-year NERS 2014.
Table 3. Mean and analysis of variance of wheat yield components, grain N and Si content, and % protein for site-year NERS 2014.
N kg ha−1Slag Mg ha−1YieldSpikeGrain per Spike1000-Grain gNSiProtein
kg ha−1Count per m2Weight gLength cm%
10106384675 ab1.496.8326.135.91.70 cd0.0239.9 cd
16517590 b1.537.0228.636.01.69 cd0.0059.9 cd
25987701 ab1.496.9824.635.61.62 e0..0329.4 e
4.56472588 b1.546.8830.936.01.65 de0.0139.6 de
96472722 a1.517.3124.336.81.65 de0.0089.6 de
mean6317 B6551.517.0126.936.11.66 B0.0169.7 B
14506931685 ab1.627.3428.635.81.76 bc0.01210.2 bc
16790660 ab1.547.1929.335.71.87 a0.05010.9 a
26971678 ab1.506.9229.535.21.75 bc0.02810.2 bc
4.57198757 a1.657.4427.235.61.79 b0.02310.4 b
96868592 b1.487.0629.736.11.75 bc0.00510.2 bc
mean6952 A6741.567.1928.935.71.78 A0.02410.4 A
Analysis of variance
Np-value0.0020.5560.3660.1340.1790.124<0.0010.347<0.001
Sip-value0.7830.7530.6720.7230.7960.1430.0230.3110.023
N × Sip-value0.8140.0800.8240.1520.2580.9540.3640.1330.367
Notes: N—nitrogen; slag—silicate slag rate. Actual p-values are reported. Values with the same uppercase (N treatment) and lowercase (across N and Si combinations) letters are not significantly different according to LSD at 0.05 level of confidence.
Table
Table 4. Mean and analysis of variance of wheat yield components, grain N and Si content, and % protein for site-year BH 2014.
Table 4. Mean and analysis of variance of wheat yield components, grain N and Si content, and % protein for site-year BH 2014.
N kg ha−1Slag Mg ha−1YieldSpikeGrain per Spike1000-Grain gNSiProtein
kg ha−1Count per m2Weight gLength cm%
10105177 c5001.767.1433.436.81.47 e0.0628.6 e
15613 c4661.747.1030.637.01.48 e0.0488.6 e
25674 c5121.817.3630.437.01.49 de0.0788.7 de
4.55472 c5141.767.5029.537.61.49 de0.0588.7 de
96492 ab4581.837.4839.837.71.61 abc0.0429.4 abc
mean5685 B490 B1.787.3232.837.21.51 B0.0588.8 B
14506519 ab5531.827.3932.037.41.57 bc0.0489.2 bc
16482 b6371.797.6828.237.51.62 ab0.0529.5 ab
26594 ab6501.757.7529.037.01.55 cd0.0429.0 cd
4.56824 a5121.837.5033.438.21.58 bc0.0459.2 bc
97112 a5711.757.5030.537.41.68 a0.0509.8 a
mean6706 A585 A1.797.5630.637.51.60 A0.0489.3 A
Analysis of variance
Np-value<0.0010.0020.8360.1030.2280.136<0.0010.109<0.001
Sip-value0.0140.5570.9960.7360.2520.104<0.0010.665<0.001
N × Sip-value0.5620.2880.7400.6990.2900.5640.6140.1950.608
N—nitrogen; slag—silicate slag rate. Actual p-values are reported. Values with the same uppercase (N treatment) and lowercase (across N and Si combinations) letters are not significantly different according to LSD at 0.05 level of confidence.
Table
Table 5. Mean and analysis of variance on soil pH and nutrient content of soil samples collected at harvest, NERS 2013.
Table 5. Mean and analysis of variance on soil pH and nutrient content of soil samples collected at harvest, NERS 2013.
N kg ha−1Slag Mg ha−1SoilNutrient Content, mg kg−1
pHSiNH4NO3PCaMgSFeZn
005.6689.92.63820024948.43762.8
00 + lime6.4879.93.63423595158.63162.9
10105.7629.82.93119684927.53362.7
15.7609.63.53320795168.33542.7
26.0839.83.33121245168.33343.0
4.56.311810.24.835247256810.03313.4
96.513810.54.937244755211.83453.2
14505.4589.73.33519874798.43892.9
15.66410.24.33920704949.23753.0
26.0789.04.13522425429.03513.2
4.56.211810.04.73823435379.43503.1
9714410.57.636264559211.23153.1
Analysis of Variance
Np-value0.5190.9660.2760.5050.07650.5450.9790.6120.0940.109
Sip-value<0.001<0.0010.488<0.0010.433<0.001<0.0010.0010.095<0.001
N × Sip-value0.1420.9780.9840.6200.7550.05610.1110.6990.1080.851
Table
Table 6. Mean and analysis of variance on soil pH and nutrient content of soil samples collected at harvest, NERS 2014.
Table 6. Mean and analysis of variance on soil pH and nutrient content of soil samples collected at harvest, NERS 2014.
N kg ha−1Slag Mg ha−1SoilNutrient Content, mg kg−1
pHSiNH4NO3CaMgZn
005.13615.31.324226033.4
00 + lime5.76214.22.231076683.9
10104.93915.52.826166303.6
15.05114.22.024936143.6
25.26014.73.027746613.8
4.55.15815.03.728546664.0
95.49315.23.931327104.6
14504.84014.32.125116093.3
14.94915.43.625766323.4
24.95215.73.626756363.6
4.55.06016.84.727516514.0
95.58214.73.829886884.1
Analysis of Variance
N Effectp-value0.5040.4310.2010.9280.4330.4670.120
Si Effectp-value0.010<0.0010.0300.9190.0090.0550.001
N × Si Effectp-value0.7660.8060.3480.9290.9440.9340.779
Table
Table 7. Mean and analysis of variance on soil pH and nutrient content of soil samples collected at harvest, BH 2014.
Table 7. Mean and analysis of variance on soil pH and nutrient content of soil samples collected at harvest, BH 2014.
N kg ha−1Slag Mg ha−1SoilNutrient Content, mg kg−1
pHSiNH4NO3CaMgSCuMnZn
005.6178.11.017133067.51.8541.7
00 + lime6.1356.41.820433337.21.9571.4
10105.6296.50.617433065.11.9641.4
15.9276.41.018563226.01.9631.5
25.9306.51.018643285.71.8651.5
4.56.2386.91.119803487.51.9681.8
96.5566.81.322543839.11.9722.0
14505.6306.70.817713085.31.8631.5
15.9276.70.919093235.62.0641.8
25.8306.61.218623356.51.8701.5
4.55.9456.71.320823577.62.0672.0
96.5957.81.822153858.82.0742.1
Analysis of Variance
N Effectp-value0.6760.0660.2420.0360.4910.5090.9140.8160.4670.115
Si Effectp-value0.006<0.0010.2880.002<0.001<0.001<0.0010.0540.009<0.001
N × Si Effectp-value0.9470.0820.6840.4390.8460.9910.8890.9000.7720.738
Table
Table 8. Record of trial establishment and field activities.
Table 8. Record of trial establishment and field activities.
LocationYear †Site-YearDate
Si ApplicationPlantingN ApplicationHarvest
St. Joseph2012NERS 20139-November-20129-November-20121-February-20137-June-2013
St. Joseph2013NERS 201414-November-201316-November-201321-February-20146-June-2014
Ben Hur2013BH 20148-November-201311-November-201322-February-201422-May-2014
† Year that the trial was established.
Table
Table 9. Chemical properties and textural class of initial soil samples collected from all the sites.
Table 9. Chemical properties and textural class of initial soil samples collected from all the sites.
Site-Year Extractable Nutrients †, mg kg−1Total §, %OM , %Textural Class
Si ‡PKCaMgSCuZnNC pH
NERS 20136213840121366041333.22.50.141.22.3silt loam5.66
NERS 201449403092212625203.31.90.161.32.1silt loam5.27
BH 201448351461833481171.81.00.140.91.5silt loam6.13
† Extractable nutrients based on Mehlich-3 extraction procedure and ICP. ‡ Silicon determined by 0.5 M acetic acid extraction procedure and molybdenum blue colorimetry. § C and N content determined by dry combustion. Organic matter based on Walkley and Black method.

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White, B.; Tubana, B.S.; Babu, T.; Mascagni, H.; Agostinho, F.; Datnoff, L.E.; Harrison, S. Effect of Silicate Slag Application on Wheat Grown Under Two Nitrogen Rates. Plants 2017, 6, 47. https://doi.org/10.3390/plants6040047

AMA Style

White B, Tubana BS, Babu T, Mascagni H, Agostinho F, Datnoff LE, Harrison S. Effect of Silicate Slag Application on Wheat Grown Under Two Nitrogen Rates. Plants. 2017; 6(4):47. https://doi.org/10.3390/plants6040047

Chicago/Turabian Style

White, Brandon, Brenda S. Tubana, Tapasya Babu, Henry Mascagni, Flavia Agostinho, Lawrence E. Datnoff, and Steve Harrison. 2017. "Effect of Silicate Slag Application on Wheat Grown Under Two Nitrogen Rates" Plants 6, no. 4: 47. https://doi.org/10.3390/plants6040047

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