Glycoside Hydrolase Activities in Cell Walls of Sclerenchyma Cells in the Inflorescence Stems of Arabidopsis thaliana Visualized in Situ
Abstract
:1. Introduction
2. Results
2.1. Biosynthesis of Different Fluorogenic Substrates
2.2. Optimization of in Situ Reaction Conditions for the Different Fluorogenic Substrates
Reaction Times | Substrates | Concentration (M) | pH | ||||
---|---|---|---|---|---|---|---|
5.0 | 5.5 | 6.0 | 6.5 | 7.0 | |||
20 min | Glc-β-Res | 9.3 × 10−5 | ++ | ++ | +++ | +++ | ++ |
Glc-Glc-β-Res | 9.3 × 10−5 | ++ | ++ | +++ | +++ | ++ | |
Gal-β-Res | 4.7 × 10−5 | ++ | +++ | +++ | ++++ | ++ | |
60 min | Glc-S-Glc-β-Res | 9.3 × 10−4 | - | - | + | + | ++ |
XXXG-β-Res | 9.3 × 10−4 | - | - | + | ++ | + |
2.3. In Situ Enzymatic Activity Distribution Observed in Real Time at the Tissue Level in Inflorescence Stems of Arabidopsis thaliana
2.4. In Situ Enzymatic Activity Observed in Real Time on the Cell Level in Interfascicular Fibers of Inflorescence Stems in Arabidopsis thaliana
3. Discussion
4. Experimental Section
4.1. Substrates Synthesis
4.2. Material and Growth Conditions
4.3. Optimization of the Enzymatic Reaction Conditions
4.3.1. The Reaction Time Optimization
4.3.2. pH Optimization
4.3.3. Substrate Concentration Optimization
5. Conclusions
Supplementary Files
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Banasiak, A.; Ibatullin, F.M.; Brumer, H.; Mellerowicz, E.J. Glycoside Hydrolase Activities in Cell Walls of Sclerenchyma Cells in the Inflorescence Stems of Arabidopsis thaliana Visualized in Situ. Plants 2014, 3, 513-525. https://doi.org/10.3390/plants3040513
Banasiak A, Ibatullin FM, Brumer H, Mellerowicz EJ. Glycoside Hydrolase Activities in Cell Walls of Sclerenchyma Cells in the Inflorescence Stems of Arabidopsis thaliana Visualized in Situ. Plants. 2014; 3(4):513-525. https://doi.org/10.3390/plants3040513
Chicago/Turabian StyleBanasiak, Alicja, Farid M. Ibatullin, Harry Brumer, and Ewa J. Mellerowicz. 2014. "Glycoside Hydrolase Activities in Cell Walls of Sclerenchyma Cells in the Inflorescence Stems of Arabidopsis thaliana Visualized in Situ" Plants 3, no. 4: 513-525. https://doi.org/10.3390/plants3040513