Anticholinesterase Activity of Methanolic Extract of Amorpha fruticosa Flowers and Isolation of Rotenoids and Putrescine and Spermidine Derivatives
Abstract
:1. Introduction
2. Results
2.1. Isolation and Identification of Chemical Compounds and Quantification of Compound 5
2.2. Inhibition of Acetylcholinesterase (AChE) and Butyrylcholinesterase (BuChE)
2.3. Molecular Docking
3. Discussion
4. Materials and Methods
4.1. General Experimental Procedures
4.2. Plant Material
4.3. Extraction and Isolation
4.4. Quantification of Compound 5 by HPLC-DAD
4.5. Anticholinesterase Activity
4.6. Molecular Docking
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compound | % Inhibition of AChE | % Inhibition of BuChE |
---|---|---|
1 | 9.1 ± 3.2 | 8.1 ± 5.9 |
2 | 1.1 ± 0.7 | 4.8 ± 3.3 |
3 | 14.1 ± 3.4 | 4.5 ± 2.5 |
4 | 26.6 ± 2.5 | 19.1 ± 2.1 |
5 | 47.9 ± 2.1 | 43.8 ± 1.9 |
6 | 17.9 ± 5.9 | 23.8 ± 5.2 |
7 | 37.9 ± 5.0 | 25.6 ± 5.6 |
8 | N.I. | 37.6 ± 2.7 |
9 | N.I. | 23.6 ± 2.6 |
10 | 22.4 ± 4.9 | 22.8 ± 5.9 |
galantamine | 82.1 ± 0.9 | 62.8 ± 0.3 |
Compound | Binding Affinity to AChE [Kcal/mol] | Binding Affinity to BuChE [Kcal/mol] |
---|---|---|
trans-trans-trans | −10.8 | −9.4 |
trans-trans-cis | −10.4 | −9.6 |
trans-cis-trans | −10.8 | −10.4 |
trans-cis-cis | −11.3 | −10.3 |
cis-trans-trans | −11.3 | −10.2 |
cis-trans-cis | −11.3 | −9.5 |
cis-cis-trans | −11.4 | −9.9 |
cis-cis-cis | −11.2 | −10.0 |
trans-trans | −9.8 | −8.4 |
trans-cis | −9.6 | −9.6 |
cis-trans | −10.0 | −8.7 |
cis-cis | −10.1 | −8.5 |
Galantamine | −10.5 | −8.6 |
Acetylcholine | −5.1 | −4.5 |
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Jankovská, D.; Jurčová, N.; Kubínová, R.; Václavík, J.; Švajdlenka, E.; Mascellani, A.; Maršík, P.; Bouzková, K.; Malaník, M. Anticholinesterase Activity of Methanolic Extract of Amorpha fruticosa Flowers and Isolation of Rotenoids and Putrescine and Spermidine Derivatives. Plants 2024, 13, 1181. https://doi.org/10.3390/plants13091181
Jankovská D, Jurčová N, Kubínová R, Václavík J, Švajdlenka E, Mascellani A, Maršík P, Bouzková K, Malaník M. Anticholinesterase Activity of Methanolic Extract of Amorpha fruticosa Flowers and Isolation of Rotenoids and Putrescine and Spermidine Derivatives. Plants. 2024; 13(9):1181. https://doi.org/10.3390/plants13091181
Chicago/Turabian StyleJankovská, Dagmar, Nikol Jurčová, Renata Kubínová, Jiří Václavík, Emil Švajdlenka, Anna Mascellani, Petr Maršík, Kateřina Bouzková, and Milan Malaník. 2024. "Anticholinesterase Activity of Methanolic Extract of Amorpha fruticosa Flowers and Isolation of Rotenoids and Putrescine and Spermidine Derivatives" Plants 13, no. 9: 1181. https://doi.org/10.3390/plants13091181