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Volume 13
Issue 7
10.3390/plants13070968
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Article

Unveiling the Biological Function of Phyllostachys edulis FBA6 (PeFBA6) through the Identification of the Fructose-1,6-Bisphosphate Aldolase Gene

by
Tiankuo Li
1,2,
Hui Li
1,2,
Chenglei Zhu
1,2,
Kebin Yang
1,2,
Zeming Lin
1,2,
Jiangfei Wang
1,2 and
Zhimin Gao
1,2,*
1
Key Laboratory of State Forestry and Grassland Administration/Beijing on Bamboo and Rattan Science and Technology, Beijing 100102, China
2
Institute of Gene Science and Industrialization for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing 100102, China
*
Author to whom correspondence should be addressed.
Plants 2024, 13(7), 968; https://doi.org/10.3390/plants13070968
Submission received: 4 February 2024 / Revised: 22 March 2024 / Accepted: 26 March 2024 / Published: 27 March 2024
(This article belongs to the Section Plant Molecular Biology)
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Abstract

Fructose-1,6-bisphosphate aldolase (FBA) is a pivotal enzyme in various metabolic pathways, including glycolysis, gluconeogenesis, and the Calvin cycle. It plays a critical role in CO2 fixation. Building on previous studies on the FBA gene family in Moso bamboo, our study revealed the biological function of PeFBA6. To identify CSN5 candidate genes, this study conducted a yeast two-hybrid library screening experiment. Subsequently, the interaction between CSN5 and PeFBA6 was verified using yeast two-hybrid and LCI experiments. This investigation uncovered evidence that FBA may undergo deubiquitination to maintain glycolytic stability. To further assess the function of PeFBA6, it was overexpressed in rice. Various parameters were determined, including the light response curve, CO2 response curve, and the levels of glucose, fructose, sucrose, and starch in the leaves of overexpressing rice. The results demonstrated that overexpressed rice exhibited a higher saturation light intensity, net photosynthetic rate, maximum carboxylation rate, respiration rate, and increased levels of glucose, fructose, and starch than wild-type rice. These findings indicated that PeFBA6 not only enhanced the photoprotection ability of rice but also improved the photosynthetic carbon metabolism. Overall, this study enhanced our understanding of the function of FBA and revealed the biological function of PeFBA6, thereby providing a foundation for the development of excellent carbon fixation bamboo varieties through breeding.
Keywords: Phyllostachys edulis; fructose 1,6-bisphosphate aldolase gene; phylogenetic analysis; protein interaction Phyllostachys edulis; fructose 1,6-bisphosphate aldolase gene; phylogenetic analysis; protein interaction

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MDPI and ACS Style

Li, T.; Li, H.; Zhu, C.; Yang, K.; Lin, Z.; Wang, J.; Gao, Z. Unveiling the Biological Function of Phyllostachys edulis FBA6 (PeFBA6) through the Identification of the Fructose-1,6-Bisphosphate Aldolase Gene. Plants 2024, 13, 968. https://doi.org/10.3390/plants13070968

AMA Style

Li T, Li H, Zhu C, Yang K, Lin Z, Wang J, Gao Z. Unveiling the Biological Function of Phyllostachys edulis FBA6 (PeFBA6) through the Identification of the Fructose-1,6-Bisphosphate Aldolase Gene. Plants. 2024; 13(7):968. https://doi.org/10.3390/plants13070968

Chicago/Turabian Style

Li, Tiankuo, Hui Li, Chenglei Zhu, Kebin Yang, Zeming Lin, Jiangfei Wang, and Zhimin Gao. 2024. "Unveiling the Biological Function of Phyllostachys edulis FBA6 (PeFBA6) through the Identification of the Fructose-1,6-Bisphosphate Aldolase Gene" Plants 13, no. 7: 968. https://doi.org/10.3390/plants13070968

APA Style

Li, T., Li, H., Zhu, C., Yang, K., Lin, Z., Wang, J., & Gao, Z. (2024). Unveiling the Biological Function of Phyllostachys edulis FBA6 (PeFBA6) through the Identification of the Fructose-1,6-Bisphosphate Aldolase Gene. Plants, 13(7), 968. https://doi.org/10.3390/plants13070968

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