Influence of Sucrose and Activated Charcoal on Phytochemistry and Vegetative Growth in Zephyranthes irwiniana (Ravenna) Nic. García (Amaryllidaceae)
Abstract
:1. Introduction
2. Results
2.1. Vegetative Growth in a Greenhouse
2.2. Vegetative Growth In Vitro and Influence of Sucrose and Activated Charcoal
2.3. Ethanolic Extract
2.4. Phytochemical Profile GC–MS
2.4.1. Alpha-Terpinyl-Acetate
2.4.2. Ethyl Linoleate
2.4.3. Clionasterol
2.4.4. Lycorine
2.5. Histolocalization of Alkaloids in Leaves and Bulblets
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Germination and Cultivation in a Greenhouse
4.3. In Vitro Germination
4.4. Micropropagation of Bulblets
4.5. Seedling Growth Analysis
4.6. Zephyranthes irwiniana Extract
4.7. GC–MS Analysis
4.8. Compounds Identification
4.9. Histolocalization of Alkaloids in the Leaf and Bulb of Zephyranthes irwiniana
4.10. Statistical and Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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(gL−1) | Treatment | Leaf nº | Root nº | Leaf le. (cm) | Root le. (cm) | Fresh biom. (g) | Dry biom. (g) | |
---|---|---|---|---|---|---|---|---|
Sucrose | 30.0 | T1-T2 | 3.78 a | 5.33 a | 20.25 a | 16.83 a | 0.44 a | 0.05 a |
45.0 | T3-T4 | 3.86 a | 7.97 b | 20.33 a | 18.19 a | 0.66 b | 0.06 a | |
60.0 | T5-T6 | 4.02 a | 10.89 c | 19.53 a | 17.64 a | 0.83 c | 0.10 b | |
AC | 0.0 | T1, T3, T5 | 3.63 a | 8.89 a | 18.31 a | 14.95 a | 0.61 a | 0.06 a |
1.0 | T2, T4, T6 | 4.15 b | 7.24 b | 21.76 b | 20.16 b | 0.68 a | 0.07 a |
AC (gL−1) | Sucrose (gL−1) | Treatment | Leaf nº | Root nº | Leaf le. (cm) | Root le. (cm) | Fresh biom. (g) | Dry biom. (g) |
---|---|---|---|---|---|---|---|---|
0.0 | 30.0 | T1 | 3.67 a | 6.11 a | 18.89 a | 13.71 a | 0.47 a | 0.04 a |
45.0 | T3 | 3.61 a | 8.33 b | 18.91 a | 16.55 b | 0.64 ab | 0.06 a | |
60.0 | T5 | 3.66 a | 12.22 c | 17.13 b | 14.59 ab | 0.71 b | 0.09 b | |
1.0 | 30.0 | T2 | 3.89 a | 4.56 a | 21.60 a | 19.96 a | 0.40 a | 0.05 a |
45.0 | T4 | 4.11 a | 7.61 b | 21.74 a | 19.83 a | 0.67 b | 0.06 a | |
60.0 | T6 | 4.44 a | 9.56 b | 21.93 a | 20.69 a | 0.96 c | 0.11 b |
AC (gL−1) | Sucrose (gL−1) | Treatment | Leaf nº | Root nº | Leaf le. (cm) | Root le. (cm) | Fresh biom. (g) | Dry biom. (g) |
---|---|---|---|---|---|---|---|---|
0.0 | 30.0 | T1 | 3.67 a | 6.11 a | 18.90 a | 13.71 a | 0.47 a | 0.04 a |
1.0 | T2 | 3.89 a | 4.56 a | 21.60 b | 19.96 b | 0.40 a | 0.05 a | |
0.0 | 45.0 | T3 | 3.61 a | 8.33 a | 18.91 a | 16.55 a | 0.64 a | 0.06 a |
1.0 | T4 | 4.11 b | 7.61 a | 21.75 b | 19.83 b | 0.67 a | 0.06 a | |
0.0 | 60.0 | T5 | 3.61 a | 12.22 a | 17.13 a | 14.59 a | 0.71 a | 0.09 a |
1.0 | T6 | 4.44 b | 9.56 b | 21.93 b | 20.69 b | 0.96 b | 0.11 a |
Treat. | %TIC | RT (min) | RI (exp) | RI (Lit) | Compound | Mass Fragmentation (Relative Intensity) |
---|---|---|---|---|---|---|
T3 (root) | 40.8 | 14.9 | 1351 | 1350 ± 3 | α-Terpenyl acetate | 121 (100), 93 (62), 43 (47), 136 (39), 67 (28), 68 (22), 107 (20), 91 (17), 79 (16), 77 (15) |
T1 (leaf) | 2.9 | 15.7 | 1390 | 1396 ± 2 | Ethyl decanoate | 88 (100), 101 (51), 70 (29), 73 (39), 157 (25), 41 (25), 61 (24), 155 (22), 60 (21), 43 (20) |
T2 (leaf) | 1.7 | 17.3 | 1499 | 1499 ± 8 | Eremophylene | 55 (100), 107 (97), 189 (96), 81 (88), 79 (82), 105 (73), 108 (57), 121 (55), 133 (54), 161 (41) |
T1 (bulb) | 3.5 | 18.3 | 1590 | 1595 ± 2 | Ethyl decanoate | 88 (100), 101 (38), 73 (26), 70 (23), 41 (21), 43 (20), 55 (18), 69 (14), 57 (13), 160 (12) |
T1 (leaf) | 4.7 | 21.4 | 1832 | 1837 ± 5 | Neophytadiene | 68 (100), 69 (70), 82 (63), 95 (56), 57 (53), 67 (49), 71 (44), 81 (40), 55 (38), 41 (36) |
T6 (bulb) | 3.7 | 22.7 | 1922 | 1926 ± 2 | Methyl palmitate | 74 (100), 87 (47), 43 (15), 75 (14), 143 (11), 227 (9), 83 (6), 59 (5), 129 (5), 171 (5) |
T2 (bulb) | 1.4 | 23.2 | 1956 | 1968 ± 7 | Palmitic acid | 60 (100), 43 (92), 41 (81), 55 (65), 129 (64), 69 (61), 83 (47), 213 (33), 185 (28), 256 (27) |
T6 (bulb) | 12.3 | 23.7 | 1990 | 1993 ± 3 | Ethyl palmitate | 88 (100), 70 (27), 43 (23), 41 (19), 55 (18), 57 (16), 69 (15), 89 (15), 157 (15), 241 (11) |
GH (root) | 4.0 | 25.2 | 2090 | 2092 ± 4 | Methyl linoleate | 81 (100), 67 (70), 68 (47), 41 (41), 82 (33), 96 (31), 69 (28), 79 (27), 64 (24), 109 (21) |
T1 (leaf) | 6.4 | 25.3 | 2095 | 2098 ± 3 | Methyl linolenate | 79 (100), 67 (83), 95 (56), 108 (48), 80 (48), 93 (47), 55 (43), 69 (28), 77 (28), 107 (26) |
GH (root) | 38.4 | 26.3 | 2156 | 2162 ± 6 | Ethyl linoleate | 67 (100), 81 (94), 95 (57), 82 (48), 55 (48), 79 (43), 41 (42), 68 (42), 69 (35), 109 (32) |
T5 (leaf) | 7.0 | 26.4 | 2163 | 2171 ± 13 | Ethyl linolenate | 79 (100), 95 (82), 81 (62), 108 (40), 41 (40), 44 (32), 55 (29), 93 (25), 149 (17), 119 (16) |
T5 (bulb) | 28.6 | 29.3 | 2353 | 2374 ± 25 | Octadecanamide | 59 (100), 72 (79), 41 (31), 55 (30), 69 (24), 43 (22), 83 (15), 67 (14), 81 (13), 126 (12) |
T5 (leaf) | 2.6 | 34.9 | 2734 | 2747 − N/A | Lycorine | 226 (100), 250 (62), 227 (52), 287 (23), 268 (19), 286 (16), 228 (10), 240 (6), 269 (6), 270 (5) |
GH (leaf) | 11.6 | 42.3 | 3310 | 3321 ± 31 | Clionasterol | 43 (100), 107 (88), 145 (82), 414 (77), 55 (77), 81 (75), 161 (72), 95 (72), 213 (68), 93 (66) |
Compound | Tests | Leaves | Bulbs |
---|---|---|---|
Alkaloid | Dragendorff | + | + |
Wagner | ++ | ++ |
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Dewes Neto, B.; Gomes-Copeland, K.K.P.; Silveira, D.; Gomes, S.M.; Craesmeyer, J.M.M.; de Castro Nizio, D.A.; Fagg, C.W. Influence of Sucrose and Activated Charcoal on Phytochemistry and Vegetative Growth in Zephyranthes irwiniana (Ravenna) Nic. García (Amaryllidaceae). Plants 2024, 13, 569. https://doi.org/10.3390/plants13050569
Dewes Neto B, Gomes-Copeland KKP, Silveira D, Gomes SM, Craesmeyer JMM, de Castro Nizio DA, Fagg CW. Influence of Sucrose and Activated Charcoal on Phytochemistry and Vegetative Growth in Zephyranthes irwiniana (Ravenna) Nic. García (Amaryllidaceae). Plants. 2024; 13(5):569. https://doi.org/10.3390/plants13050569
Chicago/Turabian StyleDewes Neto, Bertholdo, Kicia Karinne Pereira Gomes-Copeland, Dâmaris Silveira, Sueli Maria Gomes, Julia Marina Muller Craesmeyer, Daniela Aparecida de Castro Nizio, and Christopher William Fagg. 2024. "Influence of Sucrose and Activated Charcoal on Phytochemistry and Vegetative Growth in Zephyranthes irwiniana (Ravenna) Nic. García (Amaryllidaceae)" Plants 13, no. 5: 569. https://doi.org/10.3390/plants13050569