Metabolic Patterns of Flavonoid and Its Key Gene Expression Characteristics of Five Cultivars of Tulipa gesneriana during Flower Development
Abstract
:1. Introduction
2. Results
2.1. Preliminary Identification of Anthocyanidin Types in Tulips
2.2. Carotenoid Content in Tulips
2.3. Qualitative Analysis of Flavonoid in Tepals of Five Tulip Cultivars
2.3.1. Identification of the Components of Anthoxanthins
2.3.2. Identification of the Components of Anthocyanins
2.4. Metabolic Patterns of Flavonoid in Tepals of Five Tulip Cultivars
2.4.1. Metabolic Patterns of Anthoxanthins
2.4.2. Metabolic Patterns of Anthocyanins
2.4.3. Metabolic Patterns of Total Flavonoids
2.5. Expression of Key Structural Genes in the Flavonoid Synthesis Pathway
3. Discussion
3.1. Flavonoid Is the Key Metabolite Regulating Tulip Flower Colors
3.2. Key Structural Genes in the Flavonoid Pathways Regulating the Formation of Specific Flavonoids, thereby Affecting Flower Color in Tulips
4. Materials and Methods
4.1. Plant Materials
4.2. Methods
4.2.1. Color Reactions of Tepals in Five Tulips
4.2.2. Extraction and Determination of Carotenoid in Five Tulips
4.2.3. Extraction and Determination of Flavonoids in Five Tulips
4.2.4. Expression Analysis of Key Structural Genes in the Flavonoid Synthesis Pathway
4.2.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Cultivar | Petroleum Enter | 10% HCI | NH3·H2O | Pigment Type |
---|---|---|---|---|
PM | Colorless | Colorless | Light-yellow | Flavonols |
SL | Yellow | Colorless | Light-yellow | Carotenoids and flavonols |
OE | Light-yellow | Light-yellow | Yellow | Carotenoids, anthocyanin, and flavonols |
PD | Yellow | Orange | Yellow | Carotenoids, anthocyanin, and flavonols |
QN | Colorless | Red | Dark-yellow | Anthocyanin and flavonols |
No. | RT (min) | Molecular Ions (m/z) | Fragment Ions (m/z) | Identified Components | Cultivars | Reference for Identification |
---|---|---|---|---|---|---|
F1 | 3.96 | 610 | 301 | Quercetin-3-O-neohesperidoside | SL, PD, QN | [18] |
F2 | 4.41 | 461 | 285 | Kaempferol-3-glucuronide | PM, SL, OE, PD, QN | [19,20] |
F3 | 5.13 | 755 | 591,301 | Quercetin-3-galactose-sambubiose | PM, QN | [19] |
F4 | 5.24 | 447 | 285 | Kaempferol-3-O-galactoside | OE | [21] |
F5 | 5.29 | 625 | 301 | Quercetin 3- diglucoside | PM, PD | [22] |
F6 | 5.43 | 433 | 271 | Naringenin 3-glucoside | PM, SL | [18,19] |
F7 | 5.53 | 609 | 301 | Quercetin derivative | SL, QN | [20,22] |
F8 | 5.7 | 609 | 301 | Quercetin derivative | PM, SL, PD | [22] |
F9 | 5.82 | 609 | 301 | Quercetin derivative | QN | [22] |
F10 | 5.91 | 609 | 301 | Rutin | PM, SL, OE, PD, QN | Std * |
F11 | 6.01 | 431 | 269 | Apigenin 7-glucoside | PM, SL, PD, QN | [19] |
F12 | 6.18 | 463 | 301 | Quercetin 3-glucoside | PM, SL, PD, QN | [22] |
F13 | 6.38 | 463 | 302 | Hesperetin 7-O-glucoside | SL, OE | [23] |
F14 | 6.52 | 577 | 269 | Apigenin-7-rutinoside | SL, OE, PD | [18] |
F15 | 6.64 | 593 | 270 | Baicalin-7-diglucoside | PM, SL, OE, PD, QN | [18] |
F16 | 6.83 | 623 | 315 | Isorhamnetin-3-rutinoside | PM, SL, OE, PD, QN | [20,21,23] |
F17 | 6.94 | 447 | 285 | Kaempferol-3-O-glucoside | PM, SL, OE, PD, QN | [22,23] |
No. | RT (min) | Molecular Ions (m/z) | Fragment Ions (m/z) | Tentative Identification | Cultivars | Reference for Identification |
---|---|---|---|---|---|---|
A1 | 5.34 | 611 | 303 | Delphinidin 3-rutinoside | QN | [16,24] |
A2 | 5.81 | 595 | 448, 287 | Cyanidin 3-rhamnoside 5-glucoside | QN | [16] |
A3 | 6.27 | 579 | 271 | Pelargonidin 3-rutinoside | OE, PD, QN | [16] |
A4 | 7.65 | 271 | / | Pelargonidin | PD | [16] |
A5 | 8.45 | 465 | 303 | Delphinidin3-O-glucoside | QN | [16] |
A6 | 8.66 | 435 | 303 | Delphinidin 3-arabinoside | QN | [24] |
A7 | 9.14 | 433 | 271 | Pelargonidin 3-glucoside | PM, SL, OE, PD | [16,24] |
A8 | 9.23 | 611 | 464, 303 | Delphinidin 3-rhamnoside 5-glucoside | OE, PD QN | [24] |
A9 | 9.49 | 419 | 287 | Cyanidin 7-arabinoside | SL, QN | [16,24] |
A10 | 9.56 | 449 | 287 | Cyanidin 3-rutinoside | PM, SL, QN | Std * |
A11 | 9.69 | 417 | 271 | Pelargonidin 3-rhamnoside | SL, OE, PD | [16] |
A12 | 10.07 | 595 | 287 | Cyanidin 3-pelargonidin | PM, SL, OE, PD, QN | [24] |
A13 | 10.25 | 625 | 317 | Petunidin 3-rutinoside | PM | [16,24] |
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Li, S.; Chen, J.; Guo, X.; Li, X.; Shen, Q.; Fu, X.; Tang, D. Metabolic Patterns of Flavonoid and Its Key Gene Expression Characteristics of Five Cultivars of Tulipa gesneriana during Flower Development. Plants 2024, 13, 459. https://doi.org/10.3390/plants13030459
Li S, Chen J, Guo X, Li X, Shen Q, Fu X, Tang D. Metabolic Patterns of Flavonoid and Its Key Gene Expression Characteristics of Five Cultivars of Tulipa gesneriana during Flower Development. Plants. 2024; 13(3):459. https://doi.org/10.3390/plants13030459
Chicago/Turabian StyleLi, Shu, Jing Chen, Xueying Guo, Xin Li, Qiang Shen, Xueqing Fu, and Dongqin Tang. 2024. "Metabolic Patterns of Flavonoid and Its Key Gene Expression Characteristics of Five Cultivars of Tulipa gesneriana during Flower Development" Plants 13, no. 3: 459. https://doi.org/10.3390/plants13030459