Bioinformatics Analysis of the Panax ginseng Cyclophilin Gene and Its Anti-Phytophthora cactorum Activity

Zhao, Yu; Lu, Jiahong; Wang, Yuming; Hao, Kaiwen; Liu, Zhimei; Hui, Ge; Sun, Tianxia

doi:10.3390/plants13192731

Open AccessArticle

Bioinformatics Analysis of the Panax ginseng Cyclophilin Gene and Its Anti-Phytophthora cactorum Activity

by

Yu Zhao

^†,

Jiahong Lu

^†,

Yuming Wang

,

Kaiwen Hao

,

Zhimei Liu

,

Ge Hui

^* and

Tianxia Sun

^*

Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Plants 2024, 13(19), 2731; https://doi.org/10.3390/plants13192731

Submission received: 28 August 2024 / Revised: 9 September 2024 / Accepted: 18 September 2024 / Published: 29 September 2024

(This article belongs to the Special Issue Bioinformatics and Functional Genomics in Modern Plant Science)

Download

Browse Figures

Versions Notes

Abstract

In this paper, Panax ginseng cyclophilin (PgCyP) was successfully obtained through a genetic engineering technique. A bioinformatics method was used to analyze the physicochemical properties and structure of PgCyP. The results showed that PgCyP belongs to the cyclophilin gene family. The protein encoded by the PgCyP gene contains the active site of PPIase (R62, F67, and H133) and a binding site for cyclosporine A (W128). The relative molecular weight of PgCyP is 187.11 bp; its theoretical isoelectric point is 7.67, and it encodes 174 amino acids. The promoter region of PgCyP mainly contains the low-temperature environmental stress response (LTR) element, abscisic acid-responsive cis-acting element (ABRE), and light-responsive cis-acting element (G-Box). PgCyP includes a total of nine phosphorylation sites, comprising four serine phosphorylation sites, three threonine phosphorylation sites, and two tyrosine phosphorylation sites. PgCyP was recombined and expressed in vitro, and its recombinant expression was investigated. Furthermore, it was found that the recombinant PgCyP protein could effectively inhibit the germination of Phytophthora cactorum spores and the normal growth of Phytophthora cactorum mycelia in vitro. Further experiments on the roots of susceptible Arabidopsis thaliana showed that the PgCyP protein could improve the resistance of arabidopsis to Phytophthora cactorum. The findings of this study provide a basis for the use of the PgCyP protein as a new type of green biopesticide.

Keywords: Panax ginseng; cyclophilin; Phytophthora cactorum; bioinformatics; novel pesticides

Share and Cite

MDPI and ACS Style

Zhao, Y.; Lu, J.; Wang, Y.; Hao, K.; Liu, Z.; Hui, G.; Sun, T. Bioinformatics Analysis of the Panax ginseng Cyclophilin Gene and Its Anti-Phytophthora cactorum Activity. Plants 2024, 13, 2731. https://doi.org/10.3390/plants13192731

AMA Style

Zhao Y, Lu J, Wang Y, Hao K, Liu Z, Hui G, Sun T. Bioinformatics Analysis of the Panax ginseng Cyclophilin Gene and Its Anti-Phytophthora cactorum Activity. Plants. 2024; 13(19):2731. https://doi.org/10.3390/plants13192731

Chicago/Turabian Style

Zhao, Yu, Jiahong Lu, Yuming Wang, Kaiwen Hao, Zhimei Liu, Ge Hui, and Tianxia Sun. 2024. "Bioinformatics Analysis of the Panax ginseng Cyclophilin Gene and Its Anti-Phytophthora cactorum Activity" Plants 13, no. 19: 2731. https://doi.org/10.3390/plants13192731

APA Style

Zhao, Y., Lu, J., Wang, Y., Hao, K., Liu, Z., Hui, G., & Sun, T. (2024). Bioinformatics Analysis of the Panax ginseng Cyclophilin Gene and Its Anti-Phytophthora cactorum Activity. Plants, 13(19), 2731. https://doi.org/10.3390/plants13192731

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Bioinformatics Analysis of the Panax ginseng Cyclophilin Gene and Its Anti-Phytophthora cactorum Activity

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI