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Volume 13
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Article

Comparative Proteomic Analysis of Wild and Cultivated Amaranth Species Seeds by 2-DE and ESI-MS/MS

by
Esaú Bojórquez-Velázquez
1,2,
Jesus Alejandro Zamora-Briseño
2,
Alberto Barrera-Pacheco
1,
Eduardo Espitia-Rangel
3,
Alfredo Herrera-Estrella
4 and
Ana Paulina Barba de la Rosa
1,*
1
Instituto Potosino de Investigación Científica y Tecnológica A. C., San Luis Potosí 78216, Mexico
2
Red de Estudios Moleculares Avanzados, Campus III, Instituto de Ecología A. C., Xalapa 91073, Mexico
3
Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Texcoco 56250, Mexico
4
Unidad de Genómica Avanzada, CINVESTAV, Irapuato 36821, Mexico
*
Author to whom correspondence should be addressed.
Plants 2024, 13(19), 2728; https://doi.org/10.3390/plants13192728
Submission received: 8 August 2024 / Revised: 18 September 2024 / Accepted: 26 September 2024 / Published: 29 September 2024
(This article belongs to the Special Issue Plant Proteomics 2024)
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Abstract

Amaranth is a promising staple food that produces seeds with excellent nutritional quality. Although cultivated species intended for grain production have interesting agronomic traits, relatively little is known about wild species, which can prosper in diverse environments and could be a rich genetic source for crop improvement. This work focuses on the proteomic comparison between the seeds of wild and cultivated amaranth species using polarity-based protein extraction and two-dimensional gel electrophoresis. Differentially accumulated proteins (DAPs) showed changes in granule-bound starch synthases and a wide range of 11S globulin isoforms. The electrophoretic profile of these proteins suggests that they may contain significant phosphorylation as post-translational modifications (PTMs), which were confirmed via immunodetection. These PTMs may impact the physicochemical functionality of storage proteins, with potential implications for seed agronomic traits and food system applications. Low-abundant DAPs with highly variable accumulation patterns are also discussed; these were involved in diverse molecular processes, such as genic regulation, lipid storage, and stress response.
Keywords: Amaranthus; proteomics; two-dimensional gel electrophoresis; seed storage proteins; GBSSI Amaranthus; proteomics; two-dimensional gel electrophoresis; seed storage proteins; GBSSI

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MDPI and ACS Style

Bojórquez-Velázquez, E.; Zamora-Briseño, J.A.; Barrera-Pacheco, A.; Espitia-Rangel, E.; Herrera-Estrella, A.; Barba de la Rosa, A.P. Comparative Proteomic Analysis of Wild and Cultivated Amaranth Species Seeds by 2-DE and ESI-MS/MS. Plants 2024, 13, 2728. https://doi.org/10.3390/plants13192728

AMA Style

Bojórquez-Velázquez E, Zamora-Briseño JA, Barrera-Pacheco A, Espitia-Rangel E, Herrera-Estrella A, Barba de la Rosa AP. Comparative Proteomic Analysis of Wild and Cultivated Amaranth Species Seeds by 2-DE and ESI-MS/MS. Plants. 2024; 13(19):2728. https://doi.org/10.3390/plants13192728

Chicago/Turabian Style

Bojórquez-Velázquez, Esaú, Jesus Alejandro Zamora-Briseño, Alberto Barrera-Pacheco, Eduardo Espitia-Rangel, Alfredo Herrera-Estrella, and Ana Paulina Barba de la Rosa. 2024. "Comparative Proteomic Analysis of Wild and Cultivated Amaranth Species Seeds by 2-DE and ESI-MS/MS" Plants 13, no. 19: 2728. https://doi.org/10.3390/plants13192728

APA Style

Bojórquez-Velázquez, E., Zamora-Briseño, J. A., Barrera-Pacheco, A., Espitia-Rangel, E., Herrera-Estrella, A., & Barba de la Rosa, A. P. (2024). Comparative Proteomic Analysis of Wild and Cultivated Amaranth Species Seeds by 2-DE and ESI-MS/MS. Plants, 13(19), 2728. https://doi.org/10.3390/plants13192728

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