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Article

CIRCADIAN CLOCK-ASSOCIATED1 Delays Flowering by Directly Inhibiting the Transcription of BcSOC1 in Pak-choi

by
Ying He
1,2,
Dong Xiao
1,
Cheng Jiang
1,2,
Yiran Li
1,2 and
Xilin Hou
1,2,*
1
National Key Laboratory of Crop Genetics & Germplasm Innovation and Utilization, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of China, Nanjing Agricultural University, Nanjing 210095, China
2
Nanjing Suman Plasma Engineering Research Institute Co., Ltd., Nanjing 211162, China
*
Author to whom correspondence should be addressed.
Plants 2024, 13(16), 2190; https://doi.org/10.3390/plants13162190
Submission received: 1 June 2024 / Revised: 2 August 2024 / Accepted: 5 August 2024 / Published: 8 August 2024
(This article belongs to the Special Issue Floral Biology 3.0)

Abstract

Flowering is critical to the success of plant propagation. The MYB family transcription factor CIRCADIAN CLOCK-ASSOCIATED1 (CCA1) is an essential component of the core loop of the circadian clock and plays a crucial role in regulating plant flowering time. In this study, we found that photoperiod affects the expression pattern and expression level of BcCCA1, which is delayed flowering time under short-day conditions in Pak-choi [Brassica campestris (syn. Brassica rapa) ssp. chinensis]. We detected overexpression and silencing of BcCCA1 in Pak-choi, resulting in delayed and promoted flowering time, respectively. Furthermore, we also discovered that FLOWERING LOCUS C (BcFLC) and SUPPRESSOR OF CONSTANS1 (BcSOC1) were expressed significantly differently in BcCCA1 overexpression and silencing plants compared with control plants. Therefore, we further investigated the interaction relationship between BcCCA1, BcFLC, and BcSOC1, and the results showed that BcCCA1 and BcFLC as a complex interacted with each other. Moreover, both BcCCA1 and BcFLC can directly bind to the promoter of BcSOC1 and repress its transcription, and BcCCA1 can form a complex with BcFLC to enhance the transcriptional inhibition of BcSOC1 by BcFLC. This study reveals a new mechanism by which the circadian clock regulates flowering time.
Keywords: circadian clock; flowering time; BcCCA1; BcFLC; BcSOC1; Pak-choi circadian clock; flowering time; BcCCA1; BcFLC; BcSOC1; Pak-choi

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MDPI and ACS Style

He, Y.; Xiao, D.; Jiang, C.; Li, Y.; Hou, X. CIRCADIAN CLOCK-ASSOCIATED1 Delays Flowering by Directly Inhibiting the Transcription of BcSOC1 in Pak-choi. Plants 2024, 13, 2190. https://doi.org/10.3390/plants13162190

AMA Style

He Y, Xiao D, Jiang C, Li Y, Hou X. CIRCADIAN CLOCK-ASSOCIATED1 Delays Flowering by Directly Inhibiting the Transcription of BcSOC1 in Pak-choi. Plants. 2024; 13(16):2190. https://doi.org/10.3390/plants13162190

Chicago/Turabian Style

He, Ying, Dong Xiao, Cheng Jiang, Yiran Li, and Xilin Hou. 2024. "CIRCADIAN CLOCK-ASSOCIATED1 Delays Flowering by Directly Inhibiting the Transcription of BcSOC1 in Pak-choi" Plants 13, no. 16: 2190. https://doi.org/10.3390/plants13162190

APA Style

He, Y., Xiao, D., Jiang, C., Li, Y., & Hou, X. (2024). CIRCADIAN CLOCK-ASSOCIATED1 Delays Flowering by Directly Inhibiting the Transcription of BcSOC1 in Pak-choi. Plants, 13(16), 2190. https://doi.org/10.3390/plants13162190

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