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Article

Drip Fertigation Enhances the Responses of Grain Yield and Quality to Nitrogen Topdressing Rate in Irrigated Winter Wheat in North China

College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China
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Authors to whom correspondence should be addressed.
Plants 2024, 13(11), 1439; https://doi.org/10.3390/plants13111439
Submission received: 20 April 2024 / Revised: 20 May 2024 / Accepted: 20 May 2024 / Published: 22 May 2024
(This article belongs to the Section Crop Physiology and Crop Production)

Abstract

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Conventional water and nitrogen (N) management practice in north China, comprising flood irrigation and N fertilizer broadcast (FB), limits sustainable wheat production. Drip fertigation (DF) has been widely adopted in wheat production in recent years and has effectively improved yields. However, the responses of the yield and quality to the N topdressing rate (NTR) under DF are still unclear. This study determined the responses of the wheat yield and quality to NTR under DF, as well as assessing whether DF could synergistically increase the yield and quality. A field experiment was conducted in north China for two seasons (2021–2023) using a split-plot design with three replicates. The main plot used the management practice (FB and DF) and the sub-plot had N treatment (no N applied, and NTRs of 0, 40, 80, 120, and 160 kg ha−1 with 150 kg N ha−1 as basal fertilizer, denoted as N0, T0, T40, T80, T120, and T160, respectively). Our results showed that high and saturated wheat yields (12.08 and 11.46 t ha−1) were obtained under DF at T80, and the highest yields were produced at T160 (11.71 and 11.30 t ha−1) under FB. Compared with FB, the greatest yield increase of 10.4–12.6% was achieved at T80 under DF. A higher spike number due to the increased effective stem percentage and a greater grain weight because of enhanced post-anthesis biomass production (BPpost) explained the improved yield under DF. The enhanced post-anthesis radiation use efficiency (RUE) led to the greater BPpost under DF. The enhanced specific leaf N, antioxidant capacity, and stomatal conductance under DF explained the higher light-saturated photosynthesis rate of flag leaves, which partly led to the increased post-anthesis RUE. NTR higher than 80 kg ha−1 did not enhance the yield, but it significantly improved the gliadin and glutelin contents, thereby leading to a higher total protein content, better gluten characteristics, and superior processing quality. Therefore, drip fertigation is a practical strategy for increasing both yield and quality with reduced water input and appropriate N input in irrigated winter wheat in north China. Applying 80 kg ha−1 of NTR under drip irrigation produces a high yield, but further gain in grain quality needs a higher NTR.

1. Introduction

Wheat (Triticum aestivum L.) plays a vital role in food security in China and worldwide, and the area planted with wheat throughout the world and in China specifically comprises approximately 17% and 24% of the total area planted with cereal crops, respectively [1]. In north China, the total winter wheat planting area and yield in Henan, Shandong, Hebei, Shaanxi, and Shanxi provinces account for 57% and 62% of the total in China, respectively [2]. In this region, precipitation during the growing season of winter wheat could only provide 25–30% of the water requirements [3,4]. Thus, it is important to apply supplementary irrigation to ensure that the water supply is sufficient for high and stable winter wheat production. The dominant irrigation method used in wheat production in north China is conventional flood irrigation. However, the large amounts of water consumed in the conventional irrigation method lead to the inefficient utilization of limited water resources [5], which is not conducive to sustainable food production [6,7].
The application of nitrogen (N) fertilizer is also critical for the growth of wheat plants and yield formation. The leaves are fewer in number and/or smaller when the N supply is insufficient, which inhibits the canopy radiation capture and photosynthesis capacity, thereby reducing the crop grain yield and quality [8,9,10]. Appropriate N management determines increases in the wheat grain yield and quality improvements [11]. Under the conventional flood irrigation method, N fertilizer is usually broadcast on the soil surface or buried in the topsoil. After irrigation with a large amount of water, a considerable proportion of the N infiltrates into the deep layer soil, where N fertilizer cannot be absorbed sufficiently by the limited roots [12,13,14]. Water and N fertilizer management practices are characterized by flood irrigation and N fertilizer broadcast (FB), which limits the efficient use of water and N fertilizer, and it also leads to a high risk of nitrate leaching [15,16]. Therefore, it is necessary to improve water and N management practices to increase crop productivity and the efficiency of resource use, as well as to reduce negative ecological impacts.
An effective management solution is drip fertigation (DF), which can supply water and fertilizer simultaneously to the crop root zone by controlling a drip irrigation system in a timely and precise manner according to the water and nutrient requirements of crops [17,18,19]. DF has been widely used in vegetable and fruit production [20], and it has recently become increasingly applied in cereal crop production in areas with water scarcity [12,21,22]. Numerous studies have shown that DF can achieve the combined goals of reducing water usage, increasing N use efficiency, and obtaining high yields. DF can significantly increase crop yields by 10–22% compared with traditional irrigation and fertilization practices [23,24,25]. Lu et al. [14] found that compared with FB, DF increased the wheat yield, water productivity, N fertilizer use efficiency, and net income by 43–56%, 44–54%, 47–111%, and 88–100% compared with FB, respectively. Li et al. [26] analyzed 1033 publications and concluded that compared with FB, DF could increase the crop yield, water productivity, and N fertilizer use efficiency by 12%, 26%, and 34%, respectively. These studies mostly focused on the crop yield, water evapotranspiration and productivity, and N uptake and utilization, whereas few considered biomass production, translocation, and partitioning, which primarily determine the crop yield performance.
Crop yield is dependent on both biomass production and partitioning (i.e., harvest index). The grain yields from modern wheat varieties are mainly improved by increasing the accumulation of biomass, and it is difficult to further increase the harvest index by genetic improvement or enhanced cultivation [27,28]. According to crop growth analysis, two physiological traits comprising the canopy radiation interception and radiation use efficiency (RUE) have key roles in determining the crop biomass [29,30]. The speed of canopy development and closure, as well as the architecture of the canopy, determine the interception of radiation. In particular, the leaf area index (LAI) plays an important role in determining the capacity of radiation capture [31,32]. The RUE reflects the efficiency of converting the radiation interception into biomass, regarding the fundamental bottleneck that hinders improvements in wheat yields [33]. Moreover, RUE is sensitive to the leaf N conditions, especially the N content per unit leaf area (SLN, g N m2) [34,35,36]. Biomass increases can be achieved by improving one or both parameters. However, little information is available about the responses of canopy radiation interception and RUE to different water and N management practices.
Due to plant absorption and N losses by nitrate leaching and ammonia volatilization during the long winter dormancy period, the residual basal N fertilizer typically cannot meet the requirements for rapid plant growth and development. Therefore, the application of N fertilizer topdressing in the spring is widely adopted in irrigated winter wheat production. Wen et al. [37] showed that an appropriate N topdressing rate (NTR) can increase the effective stem percentage, spike number, and grain weight, and thus the grain yield. Zhao et al. [38] and Ma et al. [39] showed that the flag leaf chlorophyll content and net photosynthesis rate, total biomass, harvest index, and yield increased as the NTR increased. In addition, these studies showed that the protein content, wet gluten content, dough development, and stability time improved as NTR increased [40]. Guo et al. [41] found that the protein content, wet gluten content, and dough stability time increased continuously as the NTR increased from 0 to 180 kg ha−1, although the peak yield was achieved at 150 kg ha−1. These previous studies were all conducted under FB, whereas little information is available about how different NTRs under DF might affect the grain yield and quality. Thus, it is necessary to obtain this information in order to optimize N management strategies under DF to facilitate yield and quality improvements.
Therefore, in the present study, based on a field experiment conducted for two years, we investigated the effects of different management practices (FB and DF) and NTR on the radiation interception and RUE, biomass, yield, and quality of winter wheat in the southern Shanxi province of north China. The purposes of this study were (1) to identify the responses of the yield and quality to NTR under different management practices and (2) to clarify the underlying physiological mechanisms related to the responses according to radiation interception and RUE. We hypothesized that DF would improve both the yield and quality by strengthening their responses to NRT, and that increasing RUE would explain the more sensitive response of the yield under DF.

2. Results

2.1. Yield and Yield-Related Attributes

Averaged across N treatments, compared with FB, the grain yield increased significantly by 5.4–5.9% under DF (Figure 1). Under FB, the yield increased significantly and continuously as NTR increased from 0 to 160 kg ha−1. Under DF, the yields increased significantly and continuously as NTR increased from 0 to 80 kg ha−1, but no significant differences in the yields were found between T80, T120, and T160. There were no significant differences in the yields between management practices at N0, T0, or T160 in both seasons. Compared with FB, the yields at T40, T80, and T120 under DF increased significantly by 7.4–9.2%, 10.4–12.6%, and 6.5–6.9%, respectively. In addition, the yield at T80 under DF was significantly higher and almost equivalent to the yields at T120 and T160 under FB.
The spike number, grains per spike, and grain weight were all significantly affected by management practice, N treatment, and their interactions, except for grains per spike, which was not significantly affected by the management practice (Table 1). Averaged across N treatments, compared with FB, the spike number and grain weight increased significantly by 5.0–5.4% and 4.0–4.3% under DF, respectively, thereby resulting in higher yields under DF. As NTR increased, the spike number and grains per spike tended to increase, but the grain weight decreased. The spike number and grains per spike did not significantly increase when NTR increased from 80 to 160 kg ha−1 under DF, but significant increases were observed under FB. Similar to the yield performance, the spike number at T80 under DF was not lower than that at T160 under FB.
Rather than the maximum stem number, the higher productive stem percentage (by 6.3–6.8%) could explain the increased spike number under DF (Table 1). There were no significant differences in LAI at anthesis under the two management practices in both seasons. Under both management practices, LAI at anthesis increased significantly as NTR increased from 0 to 80 kg ha−1, but no significant increase was found as NTR increased from 80 to 160 kg ha−1.

2.2. Biomass Accumulation

Averaged across N treatments, compared with FB, the total biomass increased significantly by 5.8–6.5% under DF (Figure 2). The increase in the total biomass was due to the simultaneous improvements in BPpre and BPpost (by 3.6–4.8% and 9.6–9.8%, respectively). Similar to the trend in the yield, BPpre, BPpost, and the total biomass all increased as NTR increased from 0 to 160 kg ha−1 under FB, but significant increases were only found from 0 to 80 kg ha−1 and the biomass was unchanged from 80 to 160 kg ha−1 under DF.
Compared with FB, BPpre improved significantly at T40, T80, and T120 by 5.6–7.9%, 7.4–8.5%, and 4.0–5.4% under DF, respectively (Figure 2), where the corresponding percentages for BPpost were 11.3–11.4%, 15.7–16.7%, and 10.1–10.9%, and the corresponding percentages for the total biomass were 7.6–9.1%, 10.8–11.0%, and 6.2–7.3%. In particular, BPpost was 7.7–9.9% higher at T160 under DF compared with FB.

2.3. NSC Accumulation and Biomass Translocation and Partitioning

The NSC accumulation at anthesis, Btrans, NSC output rate, and the contribution rate of Btrans to the yield were all affected significantly by the management practice, N treatment, and their interaction, except that the management practice had no significant effect on NSC accumulation or Btrans (Table 2). Averaged across N treatments, compared with FB, the NSC output rate and contribution rate of Btrans to the yield were 10.1–11.1% and 9.1–13.7% lower under DF, respectively. HI was not significantly affected by the management practice. Under both management practices, small differences in HI were observed among all NTRs (0–160 kg ha−1) in both seasons.
There was no significant relationship between the grain yield and Btrans (Figure 3B, p > 0.05) in either year, whereas the grain yield was significantly and positively correlated with BPpost (Figure 3A, p < 0.01). BPpost was not correlated with Btrans (Figure 3C, p > 0.05). BPpost was significantly and negatively correlated with the NSC output rate (Figure 3D, p < 0.01).

2.4. PAR Interception and Use Efficiency

Averaged across N treatments, the pre-anthesis, post-anthesis, and total ISR did not differ significantly between the two management practices (Figure 4). The pre-anthesis and total ISR increased significantly as NTR increased from 0 to 80 kg ha−1 under both management practices. No significant differences were found under DF as NTR increased from 80 to 160 kg ha−1, but the pre-anthesis ISR improved significantly at T160 compared at T80 under FB. Moreover, the pre-anthesis ISR was 5.1–6.3% higher at T80 under DF than FB.
Averaged across N treatments, the pre-anthesis RUE did not differ significantly between the two management practices, but the post-anthesis and seasonal RUE were 9.5–9.6% and 4.1–4.6% higher under DF than FB, respectively (Figure 5). As NTR increased, the pre-anthesis RUE tended to increase slightly under both management practices in both years. Under FB, the post-anthesis and seasonal RUE tended to increase continuously as NTR increased from 0 to 160 kg ha−1. However, the post-anthesis and seasonal RUE did not differ significantly among T80, T120, and T160 under DF. Compared with FB, the post-anthesis RUE values were 11.0–11.6%, 16.0–16.9%, 10.7–11.3%, and 7.8–9.7% higher at T40, T80, T120, and T160 under DF, respectively. Compared with FB, the seasonal RUE values were 5.2–6.7%, 6.9–7.8%, and 4.7–5.6% higher at T40, T80, and T120 under DF, respectively.

2.5. Photosynthetic Characteristics of Flag Leaves after Anthesis

The Amax and Gs of flag leaves after anthesis decreased during the grain filling period (from 10 to 30 DAA) under all N treatments, management practices, and seasons, whereas Ci increased (Figure 6 and Figure 7). Amax and Gs increased as NTR increased from 0 to 160 kg ha−1 under FB, but there was no obvious change from 80 to 160 kg ha−1 under DF. However, Ci decreased as NTR increased from 0 to 160 kg ha−1 under FB, although obvious decreases were only found from 0 to 80 kg ha−1 under DF. There was no significant difference in Amax and Gs at anthesis between management practices under any N treatment in either season. The difference percentages between management practices in Amax and Gs became larger over time. At 10, 20, and 30 DAA, the Amax values under DF were 4.7–11.0%, 7.1–16.2%, and 21.5–36.7% higher at NTRs of 40–160 kg ha−1 than for FB, respectively. For Gs, the corresponding increase percentages were 3.1–10.4%, 8.8–16.0%, and 11.4–25.6%. However, for Ci, corresponding decrease percentages were 3.0–9.6%, 6.5–13.3%, and 9.9–16.4%.

2.6. SLN Contents of Flag Leaf after Anthesis

In the same way as Amax, the SLN contents of flag leaves after anthesis decreased over time under all N treatments, management practices, and seasons (Figure 8). The continuous improvements in the SLN contents were observed under both practices as NTR increased. The difference percentages in SLN also became larger over time. The SLN contents were 4.9–5.8%, 5.5–6.8%, 6.9–9.4%, and 8.8–11.7% higher at NTR of 40–160 kg ha−1 at anthesis, and 10, 20, and 30 DAA under DF than FB, respectively.

2.7. CAT and SOD Activities and MDA Contents of Flag Leaves after Anthesis

The CAT and SOD activities in the flag leaves decreased over time under both management practices in both seasons (Figures S1 and S2). At anthesis, there were no differences in the CAT or SOD activities between the two management practices under any N treatments. As time went by, the DF demonstrated larger and larger advantages in the CAT and SOD activities. At 30 DAA, the CAT and SOD activities were 10.7–15.3% and 7.5–13.0% higher at NTRs of 40–160 kg ha−1 under DF than FB, respectively.
The MDA contents of the flag leaves increased under both management practices in the two seasons (Figures S1 and S2). No significant difference was found in the MDA contents between management practices either at anthesis or 10 DAA under any N treatment or in any season. The MDA contents were 4.8–6.4% and 5.3–8.4% higher at 20 and 30 DAA at NTRs of 40–160 kg ha−1 under DF than FB, respectively.

2.8. Grain Protein Content and Protein Components

In both seasons, the gliadin, glutenin, and total protein content were significantly affected by the management practice, N treatment, and their interaction, but the albumin plus globulin was not affected by any factor (Table 3). Averaged across N treatments, the gliadin, glutenin, and total protein contents were 5.4–6.1%, 4.8–5.0%, and 3.9–4.1% higher under DF than FB, respectively. As NTR increased from 0 to 160 kg ha−1, the gliadin, glutenin, and total protein contents all tended to increase under both management practices and in both seasons. Under FB, no significant improvements were observed as NTR increased from 80 to 160 kg ha−1. By contrast, the gliadin, glutenin, and total protein contents were 9.6–10.9%, 7.9–8.5%, and 6.6–7.7% higher at T160 than those at T80 under DF, respectively. Furthermore, the gliadin, glutenin, and total protein contents were 7.4–12.6%, 6.8–10.4%, and 5.4–8.8% higher at NTRs of 120 and 160 kg ha−1 under DF than FB, respectively.

2.9. Grain Processing Quality

Averaged across N treatments, the wet gluten content and gluten index were 4.1–4.7% and 4.4–4.5% higher under DF than FB, respectively (Figure 9A–D). As NTR increased, the wet gluten content and gluten index increased continuously under both practices, but the increasing trends were stronger under DF. Under FB, the wet gluten content and gluten index were 15.8–16.6% and 11.1–11.4% higher at T160 than T0, respectively, but the corresponding percentages under DF were 26.6–28.9% and 21.0–21.1%. At NTRs of 120 and 160 kg ha−1, the wet gluten content and gluten index were 5.3–10.7% and 5.7–9.5% higher under DF than those under FB, respectively.
Averaged across N treatments, the dough development time and stability time were 10.3–12.0% and 10.3–13.0% higher under DF than FB, respectively (Figure 9E–H). As NTR increased, both the development time and stability time increased continuously under DF, whereas the values did not increase when NTR exceeded 80 kg ha−1 under FB. Under DF, the dough development time and stability time were 29.5–31.3% and 30.2–30.6% higher at T160 than T80, respectively. At NTRs of 120 and 160 kg ha−1, the dough development time and stability time were 14.4–31.1% and 13.7–31.7% higher under DF than FB, respectively.

3. Discussion

Nitrogen fertilizer topdressing is essential for improving wheat productivity and grain quality. Enhancing the responses of yield and quality to NTR may be one of the most effective and meaningful ways to ensure food security without very high nitrogen fertilizer input. In the present study, the average grain yield was 5.4–5.9% higher under DF compared with FB, and similar results were obtained in previous studies [14,24,26]. Importantly, our results showed that the response of the wheat yield was more sensitive to NTR under DF than FB. High yield was achieved at T80 under DF, but no obvious peak in the yield was observed as NTR increased from 0 to 160 kg ha−1 under FB. In addition, N topdressing at 80 kg ha−1 under DF produced an equivalent yield to that at N topdressing at 160 kg ha−1 under FB in both seasons (12.08 vs. 11.71 t ha−1 in 2021−2022, and 11.46 vs. 11.30 t ha−1 in 2022−2023). These results suggest that DF can strengthen the response of the grain yield to NTR to obtain high yields without requiring high N topdressing inputs to facilitate more sustainable agriculture.
Overall, the yield improvements under DF were attributed to the increases in the number of spikes per 1 m2 and grain weight. The improvement in the number of spikes per 1 m−2 under DF was due to the increase in the productive stem percentage rather than the maximum stem number, which was mainly due to conducting N topdressing in the jointing stage when the stem number had already reached the maximum value, before declining due to the fierce competition for nutrients, water, and space. The N supply plays a critical role in stem development or death [32,42,43]. It has been widely reported that DF can provide a higher available N concentration in the topsoil layer for absorption by the roots and subsequent utilization by the shoots [17,44,45]. The greater available N supply under DF could allow a higher proportion of stems to develop into productive spikes. In terms of grain production, both BPpost and Btrans determine the grain weight, and thus the grain yield [46,47]. We found that Btrans did not differ under DF and FB, but BPpost was 9.6–9.8% higher under DF. Thus, we mainly attributed the higher grain weight and yield to the higher BPpost values in both seasons. Btrans depends on the total NSC accumulation at anthesis and the NSC output rate. We found no significant increase in the total NSC accumulation under DF but a significant decrease in the NSC output rate. In addition, the NSC output rate was significantly and negatively correlated with BPpost in each year, as also shown in previous studies [48,49,50]. These results suggest that the increase in BPpost under DF was important for increasing the grain yield, but it also limited the translocation of NSC reserves into the filling grain and restricted further yield improvements. It is desirable to develop management practices or breed new cultivars that will break the negative relationship between BPpost and the NSC output to further enhance wheat yields.
The crop yield is determined by the biomass and HI. However, biomass increases have contributed more to yield improvements than HI in recent decades [27,28,51]. We found that DF increased both the pre-anthesis and post-anthesis biomass to achieve a higher total biomass, but HI was not affected. The increase in the total biomass under DF was mainly dependent on BPpost (9.6–9.8%) rather than BPpre (3.6–4.8%). The notably larger increase in BPpost under DF was attributed to the higher RUE rather than ISR. When N topdressing was conducted, the post-anthesis RUE was 7.8–16.9% higher under DF than FB. Furthermore, the post-anthesis RUE under DF at T80 was also significantly higher than or equivalent to that at T160, which produced the highest RUE under FB. These results suggest that the more sensitive response of the post-anthesis RUE mainly contributed to the greater response of the yield to NTR under DF.
Flag leaf assimilates make the most important contribution to the grain yield [52,53,54], and thus the photosynthetic capacity of flag leaves is generally used as an indicator of canopy productivity [55,56]. We found that DF significantly improved Amax, which was the result of the greater Gs of flag leaves at NTRs of 40–160 kg ha−1 from 10 to 30 DAA, whereas reduced Ci was also observed. It was reasonable to conclude that the enhanced Amax drove the improved RUE and biomass production after anthesis. The greater leaf Gs under DF allowed more CO2 to be transported into the leaf. Even so, the Ci of flag leaves under DF was lower under DF. These results indicated that DF not only strengthened CO2 assimilating capacity but also expanded the ability to obtain CO2, which was in line with Wang et al. [57]. Importantly, the values of Amax, Gs, and Ci at T80 under DF were equivalent to those at T160 under FB. This indicated that DF combined with moderate NTR could achieve high Amax and produce more photosynthetic products. Sinclair and Horie [58] reported that RUE was closely linked to the leaf carbon exchange rate (i.e., Amax), and the sensitivity of Amax to SLN is considered a potentially important source of variations in RUE. In the present study, from 10 to 30 DAA, DF consistently and significantly increased SLN at NTRs of 40–160 kg N ha−1. In addition, DF increased the CAT and SOD activities, which optimized leaf oxidation processes and protected the cells from oxidative damage, as well as reductions in the MDA content, thus improving the antioxidant capacity (Figures S2 and S3). These results indicate that DF produced a higher photosynthetic capacity in flag leaves by maintaining a better leaf N status and delaying leaf senescence after anthesis, thereby increasing the post-anthesis RUE of winter wheat.
Many studies have shown that the grain protein composition and contents and processing quality are strongly influenced by irrigation and fertilization practices [59,60,61]. The average grain protein content was significantly higher under DF than FB due to the improved availability of exogenous N and the reduced irrigation amount (decreasing from 60 to 40 mm per irrigation dose), as recognized by Coventry et al. [62] and Tari [63]. Importantly, increasing NTR was beneficial for enhancing the protein content and processing quality, as also shown by Zhao et al. [38], Ma et al. [40], and Guo et al. [41], but their responses to NTR differed between DF and FB. As NTR increased from 80 to 160 kg ha−1, the improvements in the total protein contents under DF (15.2–15.3%) were clearly greater than under FB (7.2–8.0%). The increases in the total protein content were attributed to increases in the gliadin and glutenin contents rather than the albumin and globulin contents, possibly because the accumulation of albumin and globulin depended mainly on the genotype, and the exogenous N supply had little effect [64,65]. The synthesis and accumulation of gliadin and glutenin mostly occur in the middle and late grain filling stages, and they are associated with the flag leaf N status and synthesis of free amino acids, which are determined by the available soil N supply [66]. In the present study, the SLN of flag leaves after anthesis was higher at T120 and T160 than T80 under DF. However, the higher SLN did not enhance Amax, RUE, and the biomass production after anthesis, and further increased the yield compared with T80 under DF. It is reasonable to consider that the SLN reached a critical value at T80 and that the extra gains in SLN were mainly attributable to leaf N reserves and enhanced grain protein synthesis rather than the promotion of the photosynthetic capacity [52,67,68]. Gliadin and glutelin, constituting the main body of gluten, influence the processing quality of wheat grains [69,70]. Therefore, the responses of the processing quality traits to NRT were like those of the gliadin and glutenin contents in each season. In particular, the wet gluten content, gluten index, dough development, and stability time were 9.4–31.6% higher at T160 than T80 under DF, but only 2.3–7.1% higher under FB. Thus, DF enhanced the responses of the yield and quality to NTR compared with FB. The high yield was achieved at N topdressing at only 80 kg ha−1 under DF, but further increasing the N topdressing input improved the grain protein content and processing quality.

4. Materials and Methods

4.1. Site Description

During the 2021–2022 and 2022–2023 winter wheat growing seasons, a field experiment was conducted at Gucheng Village (35°43′ N, 111°44′ E), Yicheng County, Shanxi Province, China. The study site has a typical semi-arid warm temperate continental monsoon climate, and winter wheat and summer maize rotation is the main planting system. Experiments in both growing seasons were conducted in the same field and the former crop was summer maize with normal and uniform management. Five replicate topsoil (0–30 cm layer) samples were randomly collected for the determination of the soil’s basic nutrients before sowing in 2021 and 2022. The soil type was silty clay loam in the experimental site, and the organic matter, total N, alkaline N, Olsen P, available K, and pH were 19.11–20.07 g kg−1, 0.91–1.01 g kg−1, 47.25–51.21 mg kg−1, 19.71–20.44 mg kg−1, 168.22–171.17 mg kg−1, and 8.43–8.49, respectively.
In both growing seasons, climate parameters including the daily maximum temperature, minimum temperature, precipitation, and incident solar radiation were collected during the growing period from sowing to maturity by a weather station (AWS 800, Campbell Scientific, Inc., Logan, UT, USA) located about 50 m from the experimental field. Averaged over the last two decades, the seasonal average daily mean temperature, total precipitation, and total incident solar radiation during the winter wheat growing seasons (from 15 October to 20 June) were 9.5 °C, 182.4 mm, and 2803 MJ m−2, respectively. The seasonal average daily mean temperature, total precipitation, and incident solar radiation were 10.2 and 9.1 °C, 152.8 and 214.7 mm, and 2792 and 2814 MJ m−2 in the growing seasons in 2021–2022 and 2022–2023, respectively (Figure 10). The two experimental seasons did not display obvious differences compared to the seasons of the last two decades.

4.2. Experimental Design and Field Management

A newly released and high-yielding variety (Yannong1212) of winter wheat (Triticum aestivum L.) was sown during the two consecutive wheat growing seasons. Seeds were planted by using a wide-range sowing machine (2BMYF-10/5; Yuncheng Gongli Co., Ltd., Heze, China). The sowing belt was 10 cm with row spacing of 25 cm. The sowing machine has been widely used in Shanxi province. Wheat crops were sown on 4 November 2021 and 22 October 2022. The plant densities were 400 and 300 plants m−2 in 2021–2022 and 2022–2023, respectively. In 2021, heavy rain during early October led to late winter wheat sowing, and thus the higher seed sowing rate in the first season aimed to compensate for the yield loss due to delayed sowing.
The treatments were laid out in a split-plot design. The main plot used management practices, where the practices comprised FB and DF. Both practices used flood irrigation with 60 mm of water before the wintering stage. In the 2021–2022 growing season, FB was irrigated with 60 mm in the jointing stage, booting stage, and filling stage, whereas DF was irrigated with 40 mm. In general, winter wheat has a water requirement of 400–500 mm throughout its entire growth period, but our experimental site has an average annual precipitation of only 182.4 mm. Local farmers usually irrigated 3–4 times to meet the water requirement, each time with about 60 mm. DF is a water-saving and efficient technology, so our experiment set the DF treatment to reduce the irrigation amount by 1/3 on the basis of farmers. In the 2022–2023 growing season, due to the high precipitation in May, no irrigation was applied in the filling stage under either practice. The sub-plot was N treatment, where the six N treatments comprised N0 (no N applied), T0 (no N topdressing), T40 (N topdressing at 40 kg ha−1), T80 (N topdressing at 80 kg ha−1), T120 (N topdressing at 120 kg ha−1), and T160 (N topdressing at 160 kg ha−1). Except for N0, the other N topdressing treatments received 150 kg N ha−1 (urea, 46% N), 150 kg P2O5 ha−1 (calcium superphosphate, 16% P2O5), and 90 kg K2O ha−1 (potassium chloride, 52% K2O) before sowing, and each N topdressing treatment (urea, 46% N) was conducted at the jointing stage. Each plot had a length of 10.0 m and width of 2.0 m. Twelve (2 × 6) treatments were tested and each experimental treatment was replicated three times. The specific water and N management practices during the experimental periods are shown in Table S1.
A drip irrigation system was installed after wheat emergence. The drip tapes (Φ16 mm) were arranged 50 cm apart, with one drip line serving two rows of winter wheat, and the dripper spacing was 30 cm. The drippers discharged 2.2 L h−1 at a working pressure of 0.10–0.15 MPa. A flow meter was placed in each plot to monitor the amount of irrigation water released. At the jointing stage, the weighed urea was dissolved into the fertilizer tank, a Venturi fertilizer applicator was used to suck the fertilizer into the pipeline, and then it was dripped into the field with drip tapes. The same amount of water was added to the fertilization tanks in each plot.

4.3. Sampling and Measurements

4.3.1. Grain Yield and Yield Components

At maturity, wheat spikes were cut from an area of 3.0 m2 (four typical rows with a length of 3.00 m) in the center of each plot to record the number of effective spikes that produced more than 5 grains per spike. After threshing the harvested spikes, the grains’ moisture content was measured using a digital moisture tester (PM8188A, Kett Electric Laboratory, Tokyo, Japan). The grain yield was corrected to 13.0% standard moisture content. Three 50.00 g sub-samples were weighed from the grain samples harvested in each plot to count the number of grains and to calculate the grain weight, which was also corrected to a 13.0% moisture content. The number of grains per spike was calculated as follows.
Grains   per   spike = Grain   yield Grain   weight × spike   number

4.3.2. Biomass

At anthesis and maturity, the plants with a length of 0.5 m (0.125 m2) were randomly selected in the central row of each plot for sampling. The fresh samples initially kept for 0.5 h in an oven, and were then dried at 80 °C until they reached a constant weight. The plant samples taken at maturity were divided into grains and straws. The dry weight at anthesis was the pre-anthesis biomass production (BPpre), and the total biomass at maturity was the total dry weight of grain and straw. The post-anthesis biomass production (BPpost) and biomass translocation (Btrans) were calculated as follows.
BP post   kg   ha 1 = Total   biomass BP pre
B trans   kg   ha 1 = Grain   biomass BP post
The concentration of non-structural carbohydrates (NSC; soluble sugars and starch) was determined in straw at anthesis according to Yoshida [71]. Aliquots of the soluble sugar and starch extract were assayed by anthrone reagent colorimetry using a spectrophotometer (L8, Shanghai Yidian Analytical Instrument Co., Ltd., Shanghai, China) at a wavelength of 620 nm. The starch content was calculated by multiplying the glucose content by a conversion factor of 0.9 [72].
NSC   concentration   mg / g = soluble   sugars   content + starch   content
NSC   accumulation   at   anthesis   t   ha 1 = NSC   concentration × BP pre
NSC   output   rate   % = B trans NSC   concentration × 100
The harvest index (HI) was calculated as follows.
HI   % = Yield × 0.87 Total   biomass × 100

4.3.3. Maximum Number of Stems and LAI

At the jointing stage, 1 m2 (a length of 1 m) of plants are selected from the center of each plot to count the total number of main stems and tillers (i.e., the maximum number of stems). The ratio of the spike number at maturity relative to the maximum number of stems at jointing is the percentage of effective stems. At anthesis, the wheat plants were sampled in the center of the plot with a length of 0.5 m (0.125 m2). All green leaves were separated and tiled on transparent polyethylene film to measure LAI using a leaf area meter (LI-3100C, LI-COR, Lincoln, NE, USA). LAI was calculated as follows.
LAI   m 2   m 2 = Sampled   leaf   aera Sampled   land   area

4.3.4. SLN

After measuring the LAI, the leaves were dried in an oven at 80 °C to constant weight and then crushed for measurement. The leaf N concentration (N mass per unit dry weight, mg g−1) was determined using an elemental analyzer (Rapid N Exceed, Elementar, Langenselbold, Germany). SLN (g m−2) was calculated as follows.
SLN   g   m 2 = Leaf   N   concentration × Leaf   mass Sampled   leaf   aera

4.3.5. Light Interception and Radiation Use Efficiency

The canopy PAR interception ratio was measured at an interval of 10–15 days during the growing season. The measurements were conducted between 1100 and 1300 h using a linear PAR ceptometer (AccuPAR LP-80, Decagon Devices Inc., Pullman, WA, USA). In each plot, the light bar was placed vertically to the rows and slightly above the soil surface, and then the transmitted PAR intensity was measured. After the measurement of the transmitted PAR intensity, the PAR intensity was immediately recorded above the canopy. Six pairs of PAR intensity measurements were recorded. The canopy PAR interception ratio (PARI) was calculated as follows.
PARI   % = PAR   intensity   above   canopy PAR   intensity   slightly   above   the   soil   surface   PAR   intensity   above   canopy × 100
The intercepted solar radiation (ISR) during the growth period was calculated using the average canopy PARI and accumulated incident solar radiation during the growth period, as follows.
ISR   MJ   m 2 = PARI   at   the   beginning + PARI   at   the   end   of   the   period 2 × incident   solar   radiation
The ISR during the entire growing season was the sum of the ISR values during each growth period. The RUE during one period was calculated as follows.
RUE   g   MJ 1 = Biomass ISR

4.3.6. Photosynthetic Characteristics

Light-saturated photosynthetic rate (Amax), stomatal conductance (Gs), and intercellular CO2 concentration (Ci) were measured for flag leaves using a Li-Cor LI-6400XT Portable Photosynthesis System (Licor Biosciences, Lincoln, NE, USA) at anthesis, 10 days after anthesis (10 DAA), 20 DAA, and 30 DAA. The instrument was adjusted for humidity at 50–60% within the cuvette and the settings used for obtaining the Amax readings were PAR intensity = 1500 μmol m−2 s−1, CO2 concentration in sample chamber = 360 μL L−1, and gas flow rate = 400 μmol s−1. The measurements were taken between 10:00 and 15:00 [73].

4.3.7. Catalase (CAT) and Superoxide Dismutase (SOD) Activities, and Malondialdehyde (MDA) Contents

Twenty flag leaves were sampled from each plot at anthesis, 10 DAA, 20 DAA, and 30 DAA. Fresh samples were immediately immersed in liquid N and then stored in an ultra-low temperature refrigerator at −80 °C until biochemical determination. Each fresh flag leaf was used to determine the antioxidant enzyme activities and MDA content. We used the methods described by Wang and Huang [74] to measure the CAT activity, SOD activity, and MDA content. The CAT activity was determined based on the consumption of H2O2 at 240 nm during 3 min. The SOD activity was evaluated based on its ability to inhibit the photoreduction of nitroblue tetrazolium. The MDA content was determined based on the thiobarbituric acid reaction [75].

4.3.8. Protein Content of Grains and Protein Components

Osborne protein fractions were extracted from wholemeal flour using a sequential extraction procedure according to a previous study [76]. First, one gram of wholemeal flour was weighed to extract the structural or metabolic proteins (albumin and globulin) with a mixed solution (0.067 mol L−1 HKNaPO4, pH 7.6, containing 0.4 mol L−1 NaCl, 0.067 mol L−1 Na2HPO4/KH2PO4) in a plastic centrifuge tube. The supernatant was collected as the albumin and globulin fraction after two rounds of oscillation and centrifugation. The residue in the tube was extracted three times with 70% ethanol to obtain the wholemeal flour gliadin. The upper residue was extracted with another mixed solution to obtain the final fraction of glutenin using the same procedure described above, which was repeated twice. The concentration of the wholemeal flour protein fraction was determined by using the Biuret method at 480 nm (UV-2450, Shimadzu, Kyoto, Japan).

4.3.9. Grain Quality

The wet gluten content and gluten index were determined by using a 2200 gluten washing instrument (Perten, Alpnach, Switzerland) according to standards GB/T 14608-1993 [77] and LS/T 6102-1995 [78]. The farinograph parameters (dough development time and stability time) were determined by using a FarinoGraph-E (Brabender, Duisburg, Germany) according to standard GB/T 14614-2019 [79].

4.4. Data Analysis

Statistical data analyses were performed with Statistix 9.0 (Analytical Software, Tallahassee, FL, USA). Two-way analysis of variance (ANOVA) was conducted using a mixed general linear model in each year. Treatment means were compared with the least significant difference test (α = 0.05). Differences in yields and yield-related traits under DF and FB at the same NTR were assessed using Student’s t-test (α = 0.05). Pearson’s correlation coefficients were used to analyze the relationships between traits. Plotting was performed using SigmaPlot 12.5 (Systat Software Inc., Point Richmond, CA, USA) software.

5. Conclusions

This field experiment conducted over two seasons confirmed our hypothesis that DF would enhance the responses of the yield and quality to NRT. The higher flag leaf photosynthetic capacity after anthesis due to the enhanced SLN and antioxidant capacity under DF improved the post-anthesis RUE and biomass production, thereby improving the wheat yield. The medium NTR of 80 kg ha−1 produced the highest yield under DF, but further increasing NTR enhanced the protein content, gluten characteristics, and processing quality. These findings may help to optimize N fertilizer management strategies for winter wheat production with drip fertigation in north China.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/plants13111439/s1, Table S1: Irrigation amount and nitrogen fertilization rate at different growing stages of winter wheat in the 2021–2022 and 2022–2023 growing seasons; Figure S1: The CAT and SOD activities and MDA contents of flag leaves after anthesis in the 2021–2022 growing season; Figure S2: The CAT and SOD activities and MDA contents of flag leaves after anthesis in the 2022–2023 growing season.

Author Contributions

Conceptualization, Y.W. and Z.G.; methodology, J.T. and Y.X.; software, J.T. and Y.L.; validation, J.T., Y.X. and W.L. (Wen Li); formal analysis, J.T.; investigation, J.T.; resources, J.T.; data curation, J.T.; writing—original draft preparation, J.T.; writing—review and editing, J.X., W.L. (Wen Lin), M.S., Y.W. and Z.G.; supervision, Y.W. and Z.G.; project administration, Z.G.; funding acquisition, J.T., Y.W. and Z.G. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Ministerial and Provincial Co-Innovation Centre for Endemic Crop Production with High-quality and Efficiency in Loess Plateau (SBGJXTZX-38), the Shanxi Research Fund for Outstanding Doctor (SXYBKY2020005), the Shanxi Agricultural University Scientific Research Fund (2020BQ41), the Shanxi University Technological Innovations Plan (2021L171), the Modern Agriculture Industry Technology System Construction (CARS-03-01-24), and the Shanxi Graduate Research Innovation Project (2022Y313).

Data Availability Statement

Data are contained within the article.

Acknowledgments

The authors would like to thank Zhonghua Chen for providing comments and modifications to this manuscript.

Conflicts of Interest

The authors declare no conflicts of interest.

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Figure 1. Grain yield of winter wheat in the 2021–2022 and 2022–2023 growing seasons. The different uppercase letters indicate there is a significant difference between FB and DF averaged across all nitrogen treatments according to the least significant difference test (LSD, α = 0.05). Here, * and ** indicate there is a significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent the least significant differences under FB and DF (p < 0.05), respectively.
Figure 1. Grain yield of winter wheat in the 2021–2022 and 2022–2023 growing seasons. The different uppercase letters indicate there is a significant difference between FB and DF averaged across all nitrogen treatments according to the least significant difference test (LSD, α = 0.05). Here, * and ** indicate there is a significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent the least significant differences under FB and DF (p < 0.05), respectively.
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Figure 2. Pre-anthesis, post-anthesis, and total biomass in the 2021–2022 and 2022–2023 growing seasons. The different uppercase letters indicate there is a significant difference between FB and DF averaged across all nitrogen treatments according to the least significant difference test (LSD, α = 0.05). Here, * and ** indicate there are significant differences between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
Figure 2. Pre-anthesis, post-anthesis, and total biomass in the 2021–2022 and 2022–2023 growing seasons. The different uppercase letters indicate there is a significant difference between FB and DF averaged across all nitrogen treatments according to the least significant difference test (LSD, α = 0.05). Here, * and ** indicate there are significant differences between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
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Figure 3. Relationship between yield and post-anthesis biomass (A) or biomass translocation (B), and between biomass translocation (C) or NSC output rate (D) and post-anthesis biomass.
Figure 3. Relationship between yield and post-anthesis biomass (A) or biomass translocation (B), and between biomass translocation (C) or NSC output rate (D) and post-anthesis biomass.
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Figure 4. Pre-anthesis, post-anthesis, and total ISR in the 2021–2022 and 2022–2023 growing seasons. The different uppercase letters indicate there is a significant difference between FB and DF averaged across all nitrogen treatments according to the least significant difference test (LSD, α = 0.05). Here, * indicates that there is significant difference between FB and DF according to Student’s t test at α = 0.05. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
Figure 4. Pre-anthesis, post-anthesis, and total ISR in the 2021–2022 and 2022–2023 growing seasons. The different uppercase letters indicate there is a significant difference between FB and DF averaged across all nitrogen treatments according to the least significant difference test (LSD, α = 0.05). Here, * indicates that there is significant difference between FB and DF according to Student’s t test at α = 0.05. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
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Figure 5. Pre-anthesis, post-anthesis, and total RUE in the 2021–2022 and 2022–2023 growing seasons. The different uppercase letters indicate there is a significant difference between FB and DF averaged across all nitrogen treatments according to the least significant difference test (LSD, α = 0.05). Here, * and ** indicates there is significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
Figure 5. Pre-anthesis, post-anthesis, and total RUE in the 2021–2022 and 2022–2023 growing seasons. The different uppercase letters indicate there is a significant difference between FB and DF averaged across all nitrogen treatments according to the least significant difference test (LSD, α = 0.05). Here, * and ** indicates there is significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
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Figure 6. Light-saturated photosynthetic rate (Amax), stomatal conductance (Gs), and intercellular CO2 concentration (Ci) of flag leaves after anthesis in the 2021–2022 growing season. Here, * and ** indicate that there is significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent the least significant differences under FB and DF (α = 0.05), respectively.
Figure 6. Light-saturated photosynthetic rate (Amax), stomatal conductance (Gs), and intercellular CO2 concentration (Ci) of flag leaves after anthesis in the 2021–2022 growing season. Here, * and ** indicate that there is significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent the least significant differences under FB and DF (α = 0.05), respectively.
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Figure 7. Light-saturated photosynthetic rate (Amax), stomatal conductance (Gs), and intercellular CO2 concentration (Ci) of flag leaves after anthesis in the 2022–2023 growing season. Here, * and ** indicate that there is significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
Figure 7. Light-saturated photosynthetic rate (Amax), stomatal conductance (Gs), and intercellular CO2 concentration (Ci) of flag leaves after anthesis in the 2022–2023 growing season. Here, * and ** indicate that there is significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
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Figure 8. Specific leaf N (SLN) of flag leaves after anthesis in the 2021–2022 and 2022–2023 growing seasons. Here, * and ** indicate that there is significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
Figure 8. Specific leaf N (SLN) of flag leaves after anthesis in the 2021–2022 and 2022–2023 growing seasons. Here, * and ** indicate that there is significant difference between FB and DF according to Student’s t test at α = 0.05 and 0.01, respectively. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
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Figure 9. The wet gluten content (A,B), gluten index (C,D), dough development time (E,F) and stability time (G,H) at maturity in the 2021–2022 and 2022–2023 growing seasons. Means followed by different uppercase letters are significantly different according to the least significant difference test (LSD, α = 0.05) between two management patterns. Here, ** indicates that there is significant difference between FB and DF according to Student’s t test at α = 0.05. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
Figure 9. The wet gluten content (A,B), gluten index (C,D), dough development time (E,F) and stability time (G,H) at maturity in the 2021–2022 and 2022–2023 growing seasons. Means followed by different uppercase letters are significantly different according to the least significant difference test (LSD, α = 0.05) between two management patterns. Here, ** indicates that there is significant difference between FB and DF according to Student’s t test at α = 0.05. The blue and red vertical bars represent least significant differences under FB and DF (α = 0.05), respectively.
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Figure 10. Daily mean temperatures, solar radiation, and precipitation recorded from sowing to maturity in the 2021–2022 and 2022–2023 growing seasons. The upward arrow indicates the date of anthesis. SADMT, seasonal average daily mean temperature; TP, total precipitation; TSR, total solar radiation.
Figure 10. Daily mean temperatures, solar radiation, and precipitation recorded from sowing to maturity in the 2021–2022 and 2022–2023 growing seasons. The upward arrow indicates the date of anthesis. SADMT, seasonal average daily mean temperature; TP, total precipitation; TSR, total solar radiation.
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Table 1. Yield components, maximum stem number, productive stem percentage, and leaf area index (LAI) at the anthesis of winter wheat in the 2021–2022 and 2022–2023 growing seasons.
Table 1. Yield components, maximum stem number, productive stem percentage, and leaf area index (LAI) at the anthesis of winter wheat in the 2021–2022 and 2022–2023 growing seasons.
Growing SeasonManagement PatternN
Treatment
Spikes NumberGrains per SpikeGrain WeightMaximum Stem NumberProductive Stem PercentageLAI at
Anthesis
(m–2) (mg)(m–2)(%)(m2 m–2)
2021–2022FBN0435.0 e29.9 e53.9 a1356 b32.1 a4.21 d
T0517.6 d34.4 d51.3 b1985 a26.1 d5.55 c
T40555.8 c36.6 c49.9 c1973 a28.2 c6.06 b
T80590.2 b37.6 b49.3 cd1986 a29.7 b6.47 a
T120611.6 ab38.5 ab48.6 d1927 a31.7 a6.56 a
T160620.0 a39.2 a48.2 d1989 a31.2 ab6.62 a
Mean555.0 B36.0 A50.2 B1869 A29.8 B5.91 A
DFN0439.0 d29.0 d54.6 a1319 b33.3 a4.15 d
T0531.7 c33.4 c52.4 ab1965 a27.1 c5.70 c
T40589.3 b35.0 b52.8 b1912 a30.8 b6.26 b
T80634.3 a36.4 a52.4 b1927 a32.9 a6.78 a
T120649.3 a36.5 a51.5 c1909 a34.0 a6.84 a
T160653.0 a37.2 a50.2 d1974 a33.1 a6.83 a
Mean582.8 A34.6 A52.3 A1834 A31.5 A6.09 A
ANOVA
P **ns**ns**ns
N ************
P × N *****ns*ns
2022–2023FBN0460.0 e28.7 e50.9 a1499 b30.7 a4.30 e
T0552.5 d32.2 d48.1 b2190 a25.2 d5.73 d
T40586.1 c34.0 c46.9 c2213 a26.5 c6.16 c
T80611.8 b36.6 b45.5 d2197 a27.9 b6.62 a
T120637.8 ab38.0 a45.0 d2196 a29.0 ab6.74 a
T160657.4 a38.5 a44.7 d2181 a30.1 a6.90 a
Mean583.3 B34.7 A46.9 B2079 A28.2 B6.07 A
DFN0457.7 d28.8 d51.1 a1506 b30.4 b4.27 d
T0572.1 c30.8 c49.4 b2210 a25.9 d5.89 c
T40628.6 b33.2 b48.9 b2162 a29.1 c6.41 b
T80671.0 a35.2 a48.5 b2160 a31.1 ab6.85 a
T120676.5 a36.0 a47.8 c2151 a31.4 ab6.95 a
T160688.6 a36.1 a46.7 d2132 a32.3 a7.03 a
Mean615.7 A33.4 A48.7 A2053 A30.0 A6.23 A
ANOVA
P **ns**ns**ns
N ************
P × N *****ns*ns
Within a column for each growing season, means followed by different uppercase letters are significantly different according to the least significant difference test (LSD, α = 0.05) between two management patterns. Within a column for management pattern, means followed by different lowercase letters are significantly different according to the least significant difference test (LSD, α = 0.05) among six N treatments in each season. Here, * and ** indicate significance at p = 0.05 and 0.01, respectively; ns is insignificant at p = 0.05. FB and DF refer to flood irrigation and broadcast fertilizer and drip fertigation, respectively. N0, T0, T40, T80, T120, and T160 indicate the N omission and N topdressing rates of 0, 40, 80, 120 and 160 kg ha−1, respectively.
Table 2. Translocation and accumulation of pre-anthesis non-structural carbohydrate (NSC), biomass, and harvest index of winter wheat in the 2021–2022 and 2022–2023 growing seasons.
Table 2. Translocation and accumulation of pre-anthesis non-structural carbohydrate (NSC), biomass, and harvest index of winter wheat in the 2021–2022 and 2022–2023 growing seasons.
Growing SeasonManagement PatternN
Treatment
NSC Accumulation at AnthesisBiomass TranslocationNSC Output RateThe Contribution Rate of Biomass Translocation to GrainHarvest
Index
(t ha–1)(t ha–1)(%)(%)(%)
2021–2022FBN02.13 e1.47 b69.2 a24.2 a46.9 a
T03.09 d2.02 a65.3 b25.5 a46.8 a
T403.59 c2.19 a60.9 c24.7 a46.6 a
T804.07 b2.05 a50.2 d21.5 b46.1 ab
T1204.46 a1.99 a44.8 e20.0 b45.8 ab
T1604.66 a2.06 a44.1 e20.2 b45.3 b
Mean3.67 A1.96 A55.8 A22.7 A46.2 A
DFN01.97 e1.33 c67.5 a22.1 a46.1 a
T03.14 d1.92 a60.6 b23.4 a46.5 a
T403.82 c2.09 a54.6 c22.0 a46.5 a
T804.43 b1.79 ab40.3 d17.0 b45.9 a
T1204.63 ab1.83 ab39.5 d17.2 b45.9 a
T1604.77 a1.68 b35.2 e15.8 b45.3 a
Mean3.79 A1.77 B49.6 B19.6 B46.0 A
ANOVA
P ns*****ns
N *********
P × N ********ns
2022–2023FBN02.67 f1.60 b59.8 a27.3 a48.1 a
T03.42 e2.00 a58.4 a26.8 a46.5 b
T404.01 d2.03 a50.6 b24.9 b45.4 bc
T804.51 c1.96 a43.4 c22.1 c44.4 c
T1204.88 b2.11 a43.2 c22.2 c45.0 bc
T1605.17 a2.17 a41.9 c22.0 c44.8 bc
Mean4.11 A1.98 A49.5 A24.2 A45.7 A
DFN02.69 e1.52 b54.2 a25.7 a47.7 a
T03.53 d1.95 a51.6 b25.3 a46.3 b
T404.36 c2.08 a47.7 c23.4 b45.4 bc
T804.96 b1.99 a40.1 d20.0 c45.1 c
T1205.11 ab1.96 a38.3 de19.3 c44.8 c
T1605.30 a1.86 a35.0 e18.3 d44.5 c
Mean4.33 A1.89 A44.5 B22.0 B45.6 A
ANOVA
P nsns****ns
N **********
P × N ********ns
Within a column for each growing season, means followed by different uppercase letters are significantly different according to the least significant difference test (LSD, α = 0.05) between two management patterns. Within a column for management pattern, means followed by different lowercase letters are significantly different according to the least significant difference test (LSD, α = 0.05) among six N treatments in each season. Here, * and ** indicate significance at p = 0.05 and 0.01, respectively; ns is insignificant at p = 0.05. FB and DF refer to flood irrigation and broadcast fertilizer and drip fertigation, respectively. N0, T0, T40, T80, T120, and T160 indicate the N omission and N topdressing rates of 0, 40, 80, 120, and 160 kg ha−1, respectively.
Table 3. Grain protein contents and protein components of winter wheat in the 2021–2022 and 2022–2023 growing seasons.
Table 3. Grain protein contents and protein components of winter wheat in the 2021–2022 and 2022–2023 growing seasons.
Growing
Season
Management
Pattern
N
Treatment
Protein Components (%)Total Protein
(%)
Albumin + GlobulinGliadinGlutenin
2021–2022FBN03.18 a3.62 c4.86 c11.66 c
T03.22 a3.81 bc5.04 bc12.07 bc
T403.24 a4.03 ab5.26 ab12.53 ab
T803.26 a4.16 a5.39 a12.81 a
T1203.28 a4.20 a5.43 a12.91 a
T1603.27 a4.21 a5.46 a12.94 a
Mean3.25 A4.08 B5.32 B12.65 B
DFN03.22 a3.70 e4.95 d11.87 e
T03.26 a3.87 de5.05 d12.18 de
T403.25 a4.09 cd5.39 c12.73 cd
T803.27 a4.31 bc5.59 bc13.17 bc
T1203.30 a4.51 ab5.80 ab13.61 ab
T1603.28 a4.72 a6.03 a14.03 a
Mean3.27 A4.30 A5.57 A13.15 A
ANOVA
P ns******
N ns******
P × N ns******
2022–2023FBN02.95 a3.52 c4.57 c11.04 c
T03.00 a3.71 bc4.78 bc11.49 bc
T403.03 a3.88 ab5.00 ab11.91 ab
T803.05 a4.03 a5.15 a12.23 a
T1203.07 a4.07 a5.20 a12.34 a
T1603.06 a4.11 a5.24 a12.41 a
Mean3.04 A3.96 B5.07 B12.08 B
DFN02.98 a3.60 e4.66 e11.24 e
T03.00 a3.80 de4.90 de11.71 de
T403.01 a4.01 cd5.08 cd12.09 cd
T803.05 a4.18 bc5.31 bc12.54 bc
T1203.07 a4.39 b5.57 ab13.03 ab
T1603.11 a4.63 a5.76 a13.50 a
Mean3.05 A4.20 A5.33 A12.58 A
ANOVA
P ns******
N ns******
P × N ns******
Within a column for each growing season, means followed by different uppercase letters are significantly different according to the least significant difference test (LSD, α = 0.05) between two management patterns. Within a column for management pattern, means followed by different lowercase letters are significantly different according to the least significant difference test (LSD, α = 0.05) among six N treatments in each season. Here, ** is significant at p = 0.05; ns is insignificant at p = 0.05. FB and DF refer to flood irrigation and broadcast fertilizer and drip fertigation, respectively. N0, T0, T40, T80, T120, and T160 indicate the N omission and N topdressing rates of 0, 40, 80, 120, and 160 kg ha−1, respectively.
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Tong, J.; Xiong, Y.; Lu, Y.; Li, W.; Lin, W.; Xue, J.; Sun, M.; Wang, Y.; Gao, Z. Drip Fertigation Enhances the Responses of Grain Yield and Quality to Nitrogen Topdressing Rate in Irrigated Winter Wheat in North China. Plants 2024, 13, 1439. https://doi.org/10.3390/plants13111439

AMA Style

Tong J, Xiong Y, Lu Y, Li W, Lin W, Xue J, Sun M, Wang Y, Gao Z. Drip Fertigation Enhances the Responses of Grain Yield and Quality to Nitrogen Topdressing Rate in Irrigated Winter Wheat in North China. Plants. 2024; 13(11):1439. https://doi.org/10.3390/plants13111439

Chicago/Turabian Style

Tong, Jin, Yulei Xiong, Yu Lu, Wen Li, Wen Lin, Jianfu Xue, Min Sun, Yuechao Wang, and Zhiqiang Gao. 2024. "Drip Fertigation Enhances the Responses of Grain Yield and Quality to Nitrogen Topdressing Rate in Irrigated Winter Wheat in North China" Plants 13, no. 11: 1439. https://doi.org/10.3390/plants13111439

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