MtWOX2 and MtWOX9-1 Effects on the Embryogenic Callus Transcriptome in Medicago truncatula

Krasnoperova, Elizaveta Y.; Tvorogova, Varvara E.; Smirnov, Kirill V.; Efremova, Elena P.; Potsenkovskaia, Elina A.; Artemiuk, Anastasia M.; Konstantinov, Zakhar S.; Simonova, Veronika Y.; Brynchikova, Anna V.; Yakovleva, Daria V.; Pavlova, Daria B.; Lutova, Ludmila A.

doi:10.3390/plants13010102

Open AccessArticle

MtWOX2 and MtWOX9-1 Effects on the Embryogenic Callus Transcriptome in Medicago truncatula

by

Elizaveta Y. Krasnoperova

¹

,

Varvara E. Tvorogova

^1,2,3,*

,

Kirill V. Smirnov

⁴

,

Elena P. Efremova

¹

,

Elina A. Potsenkovskaia

^1,2,3

,

Anastasia M. Artemiuk

¹

,

Zakhar S. Konstantinov

²

,

Veronika Y. Simonova

²

,

Anna V. Brynchikova

²,

Daria V. Yakovleva

¹,

Daria B. Pavlova

¹ and

Ludmila A. Lutova

^1,2

¹

Department of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Emb, 199034 St. Petersburg, Russia

²

Plant Biology and Biotechnology Department, Sirius University of Science and Technology, 1 Olympic Avenue, 354340 Sochi, Russia

³

Center for Genetic Technologies, N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 42 Bolshaya Morskaya Street, 190000 St. Petersburg, Russia

⁴

All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chausse 3, Pushkin, 196608 St. Petersburg, Russia

^*

Author to whom correspondence should be addressed.

Plants 2024, 13(1), 102; https://doi.org/10.3390/plants13010102

Submission received: 1 December 2023 / Revised: 19 December 2023 / Accepted: 27 December 2023 / Published: 28 December 2023

(This article belongs to the Special Issue Plant Meristems:The Cradle of Life)

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Abstract

WOX family transcription factors are well-known regulators of plant development, controlling cell proliferation and differentiation in diverse organs and tissues. Several WOX genes have been shown to participate in regeneration processes which take place in plant cell cultures in vitro, but the effects of most of them on tissue culture development have not been discovered yet. In this study, we evaluated the effects of MtWOX2 gene overexpression on the embryogenic callus development and transcriptomic state in Medicago truncatula. According to our results, overexpression of MtWOX2 leads to an increase in callus weight. Furthermore, transcriptomic changes in MtWOX2 overexpressing calli are, to a large extent, opposite to the changes caused by overexpression of MtWOX9-1, a somatic embryogenesis stimulator. These results add new information about the mechanisms of interaction between different WOX genes and can be useful for the search of new regeneration regulators.

Keywords: transcription factors; WOX; callus; somatic embryogenesis

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MDPI and ACS Style

Krasnoperova, E.Y.; Tvorogova, V.E.; Smirnov, K.V.; Efremova, E.P.; Potsenkovskaia, E.A.; Artemiuk, A.M.; Konstantinov, Z.S.; Simonova, V.Y.; Brynchikova, A.V.; Yakovleva, D.V.; et al. MtWOX2 and MtWOX9-1 Effects on the Embryogenic Callus Transcriptome in Medicago truncatula. Plants 2024, 13, 102. https://doi.org/10.3390/plants13010102

AMA Style

Krasnoperova EY, Tvorogova VE, Smirnov KV, Efremova EP, Potsenkovskaia EA, Artemiuk AM, Konstantinov ZS, Simonova VY, Brynchikova AV, Yakovleva DV, et al. MtWOX2 and MtWOX9-1 Effects on the Embryogenic Callus Transcriptome in Medicago truncatula. Plants. 2024; 13(1):102. https://doi.org/10.3390/plants13010102

Chicago/Turabian Style

Krasnoperova, Elizaveta Y., Varvara E. Tvorogova, Kirill V. Smirnov, Elena P. Efremova, Elina A. Potsenkovskaia, Anastasia M. Artemiuk, Zakhar S. Konstantinov, Veronika Y. Simonova, Anna V. Brynchikova, Daria V. Yakovleva, and et al. 2024. "MtWOX2 and MtWOX9-1 Effects on the Embryogenic Callus Transcriptome in Medicago truncatula" Plants 13, no. 1: 102. https://doi.org/10.3390/plants13010102

APA Style

Krasnoperova, E. Y., Tvorogova, V. E., Smirnov, K. V., Efremova, E. P., Potsenkovskaia, E. A., Artemiuk, A. M., Konstantinov, Z. S., Simonova, V. Y., Brynchikova, A. V., Yakovleva, D. V., Pavlova, D. B., & Lutova, L. A. (2024). MtWOX2 and MtWOX9-1 Effects on the Embryogenic Callus Transcriptome in Medicago truncatula. Plants, 13(1), 102. https://doi.org/10.3390/plants13010102

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MtWOX2 and MtWOX9-1 Effects on the Embryogenic Callus Transcriptome in Medicago truncatula

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