Relocation of Sr48 to Chromosome 2D Using an Alternative Mapping Population and Development of a Closely Linked Marker Using Diverse Molecular Technologies
, Jianbo Li 1, Mandeep Randhawa 1,4, Peng Zhang 1, Kerrie Forrest 5, Urmil Bansal 1,* and Harbans Bariana 1,6,*Round 1
Reviewer 1 Report
In my opinion, the manuscript entitled “Relocation of Sr48 to chromosome 2D using an alternative mapping population and development of a closely linked marker using diverse molecular technologies” is well written can be accepted in the present form. All applied methods are used according to high scientific standards.
Please pay attention that the format of the graphs and tables should be clear and unambiguous.
Reviewer 2 Report
This study is an important contribution to wheat breeding and genetics research. The researchers were able to map the Sr48 gene, which provides resistance to the devastating Ug99 stem rust disease, to chromosome 2DS of the Arina wheat variety using a DArTseq map and identify closely linked markers that can be used for marker-assisted selection of Sr48. Additionally, the study provided insights into the chromosomal translocation that led to the pseudo-linkage between Sr48 and Yr1 in the Arina/Forno population. The development of sunKASP_239 marker for Sr48 can potentially benefit wheat breeding programs globally. Overall, this study provides valuable information and tools that can be used to enhance wheat production and mitigate the threat of Ug99 stem rust disease.
I think the manuscript can be accepted.
