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Article

Two Newly Identified Colletotrichum Species Associated with Mango Anthracnose in Central Thailand

by
Chainarong Rattanakreetakul
1,
Pisut Keawmanee
1,
Santiti Bincader
2,
Orarat Mongkolporn
3,
Vipaporn Phuntumart
4,
Sotaro Chiba
5 and
Ratiya Pongpisutta
1,*
1
Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand
2
Program Plant Science, Agricultural Technology and Agro-Industry Faculty, Rajamangala University of Technology Suvarnabhumi, Phra Nakhon Si Ayutthaya 13000, Thailand
3
Department of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand
4
Department of Biological Sciences, 129 Life Sciences Building, Bowling Green State University, Bowling Green, OH 43403, USA
5
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
*
Author to whom correspondence should be addressed.
Plants 2023, 12(5), 1130; https://doi.org/10.3390/plants12051130
Submission received: 6 December 2022 / Revised: 24 February 2023 / Accepted: 26 February 2023 / Published: 2 March 2023
(This article belongs to the Section Plant Protection and Biotic Interactions)
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Abstract

Anthracnose caused by Colletotrichum spp. is one of the major problems in mango production worldwide, including Thailand. All mango cultivars are susceptible, but Nam Dok Mai See Thong (NDMST) is the most vulnerable. Through a single spore isolation method, a total of 37 isolates of Colletotrichum spp. were obtained from NDMST showing anthracnose symptoms. Identification was performed using a combination of morphology characteristics, Koch’s postulates, and phylogenetic analysis. The pathogenicity assay and Koch’s postulates on leaves and fruit confirmed that all Colletotrichum spp. tested were causal agents of mango anthracnose. Multilocus analysis using DNA sequences of internal transcribed spacer (ITS) regions, β-tubulin (TUB2), actin (ACT), and chitin synthase (CHS-1) was performed for molecular identification. Two concatenated phylogenetic trees were constructed using either two-loci of ITS and TUB2, or four-loci of ITS, TUB2, ACT, and CHS-1. Both phylogenetic trees were indistinguishable and showed that these 37 isolates belong to C. acutatum, C. asianum, C. gloeosporioides, and C. siamense. Our results indicated that using at least two loci of ITS and TUB2, were sufficient to infer Colletotrichum species complexes. Of 37 isolates, C. gloeosporioides was the most dominant species (19 isolates), followed by C. asianum (10 isolates), C. acutatum (5 isolates), and C. siamense (3 isolates). In Thailand, C. gloeosporioides and C. acutatum have been reported to cause anthracnose in mango, however, this is the first report of C. asianum and C. siamense associated with mango anthracnose in central Thailand.
Keywords: Colletotrichum; Mangifera indica L.; species identification; multilocus phylogeny Colletotrichum; Mangifera indica L.; species identification; multilocus phylogeny

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MDPI and ACS Style

Rattanakreetakul, C.; Keawmanee, P.; Bincader, S.; Mongkolporn, O.; Phuntumart, V.; Chiba, S.; Pongpisutta, R. Two Newly Identified Colletotrichum Species Associated with Mango Anthracnose in Central Thailand. Plants 2023, 12, 1130. https://doi.org/10.3390/plants12051130

AMA Style

Rattanakreetakul C, Keawmanee P, Bincader S, Mongkolporn O, Phuntumart V, Chiba S, Pongpisutta R. Two Newly Identified Colletotrichum Species Associated with Mango Anthracnose in Central Thailand. Plants. 2023; 12(5):1130. https://doi.org/10.3390/plants12051130

Chicago/Turabian Style

Rattanakreetakul, Chainarong, Pisut Keawmanee, Santiti Bincader, Orarat Mongkolporn, Vipaporn Phuntumart, Sotaro Chiba, and Ratiya Pongpisutta. 2023. "Two Newly Identified Colletotrichum Species Associated with Mango Anthracnose in Central Thailand" Plants 12, no. 5: 1130. https://doi.org/10.3390/plants12051130

APA Style

Rattanakreetakul, C., Keawmanee, P., Bincader, S., Mongkolporn, O., Phuntumart, V., Chiba, S., & Pongpisutta, R. (2023). Two Newly Identified Colletotrichum Species Associated with Mango Anthracnose in Central Thailand. Plants, 12(5), 1130. https://doi.org/10.3390/plants12051130

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