Next Article in Journal
Comprehensive Genome-Wide Identification of the RNA-Binding Glycine-Rich Gene Family and Expression Profiling under Abiotic Stress in Brassica oleracea
Next Article in Special Issue
Comparative Characterization of Pseudoroegneria libanotica and Pseudoroegneria tauri Based on Their Repeatome Peculiarities
Previous Article in Journal
New Insights in the Detection and Management of Anthracnose Diseases in Strawberries
Previous Article in Special Issue
Oligonucleotide Fluorescence In Situ Hybridization: An Efficient Chromosome Painting Method in Plants
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Plants
Volume 12
Issue 21
10.3390/plants12213705
plants-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH

by
Fei Qi
1,†,
Shuang Liang
2,†,
Piyi Xing
1,2,†,
Yinguang Bao
1,2,
Richard R.-C. Wang
3,* and
Xingfeng Li
1,2,*
1
State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China
2
Agronomy College, Shandong Agricultural University, Tai’an 271018, China
3
USDA-ARS Forage & Range Research Laboratory (FRRL), Logan, UT 84322-6300, USA
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Plants 2023, 12(21), 3705; https://doi.org/10.3390/plants12213705
Submission received: 22 September 2023 / Revised: 13 October 2023 / Accepted: 20 October 2023 / Published: 27 October 2023
(This article belongs to the Special Issue Plant Molecular Cytogenetics)
Browse Figures
Review Reports Versions Notes

Abstract

:
The genome composition of intermediate wheatgrass (IWG) is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of Th. intermedium and its relative diploid species. St2-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum were used as probes in fluorescence in situ hybridization (FISH) to classify the chromosomes of Th. intermedium into three groups, expressed as JvsJvsJrJrStSt. A combined multiplex oligonucleotide probe, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6, was used to establish the FISH karyotype of ten accessions of Th. intermedium. Variability among and within the studied accessions of intermediate wheatgrass was observed in their FISH patterns. Results of this study led to the conclusions that Jvs had largely been contributed from Da. breviaristatum, but not the present-day Da. villosum; IWG had only one J genome, Jr, which was related to either Th. elongatum or Th. bessarabicum; and St was contributed from the genus Pseudoroegneria by hybridization with Th. junceiforme or Th. sartorii.

1. Introduction

Intermediate wheatgrass (IWG; Thinopyrum intermedium (Host) Barkworth & D.R. Dewey; 2n = 6x = 42) is an important worldwide forage crop and a valuable gene reservoir for wheat improvement [1,2,3]. It has contributed many desirable traits such as resistance to three wheat rust diseases (leaf, stem, and stripe rust), barley yellow dwarf virus (BYDV), and wheat streak mosaic virus (WSMV), and grain quality for wheat improvement [3,4,5,6,7,8]. The IWG has also been developed into a perennial grain crop, named “Kernza” [9,10,11]. Therefore, IWG had been the subject of investigations by numerous researchers for a long time.
The genome constitution of IWG had been proposed as E1E2X [12], JeJeS [12] (while the symbol S was later changed to St [13]). The presence of the St genome of Pseudoroegneria in IWG was substantiated by all subsequent studies [14,15,16,17]. Using St genomic DNA as a probe, there were eight to ten chromosomes showing the signals at centromeres [14,15,16,18,19], which was given the symbol Js [15] even though it was not a complete set of 14 chromosomes. Later, St2-80 was developed as a new FISH marker for the St genome, useful in genome analysis of Triticeae species and hybrids [20]. When genomic DNA of Dasypyrum villosum or an oligo DNA from D. breviaristatum (H. Lindb.) Fred. (syn. Dasypyrum hordeaceum (Hack.) P. Candargy) pDb12H [21,22] was used as a probe, fourteen chromosomes, including those eight to ten Js type chromosomes, showed hybridization signals. On the other hand, the 14 J chromosomes were distinguished from Js and St chromosomes by a long terminal repeat (LTR) sequence pMD232-500, from Secale [18]. Therefore, Js was changed to Jvs and J was changed to Jr, and JrJrJvsJvsStSt was proposed to be the genome symbol for Th. intermedium [23]. Hence, this genome constitution had been adopted by other researchers [24,25]. It should be noted that Jr was proposed as an ancestral genome to the present-day Jb and Je genome in the diploid species Th. bessarabicum (Savul. and Rayss) A. Löve and Th. elongatum (Host) D. Dewey, respectively [23], based on the relationships between R and the J genomes revealed by restriction site differences [26].
Genetic resources for IWG have been generated, including EST-SSR markers [15], the first consensus genetic map using genotyping-by-sequencing [27], QTL mapping [28], draft genome sequence [29], and STS marker sets for the three genomes of IWG [30]. Molecular markers for disease resistance were also developed and located on various IWG chromosomes [31,32,33].
Genomic in situ hybridization (GISH) had been a valuable cytogenetic technique widely used to determine genome constitutions of plant species. However, the oligonucleotide fluorescence in situ hybridization (oFISH) could pinpoint the chromosomal locations of known genes whose DNA sequences were used as probes.
Karyotypes of oligonucleotide fluorescence in situ hybridization, coupled with molecular markers, would be useful for aiding the precise identification of individual chromosomes in IWG. Using dual and multiplex oligonucleotide FISH, we studied ten accessions of IWG having widely different origins along with IWG-related or progenitor species of the ploidy ranging from diploid to hexaploid. The objectives are (1) to test if pDb12H and St2-80 are sufficient to distinguish Jr, Jvs, and St in Thinopyrum species, (2) to determine if variability in IWG can be visualized with oFISH, and (3) to refine the relationships between IWG and its progenitor diploid species. Results will provide a clear picture of the relationships among studied species in the tribe Triticeae.

2. Results

2.1. OligoFISH of Ten Accessions of IWG

In our study, chromosomes in ten accessions of IWG (Table 1) were probed with two oligonucleotides, pDb12H and St2-80, to distinguish the Jr, Jvs, and St genomes. Then, the same chromosome spreads were probed with bulked oligonucleotides consisting of pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6 after the slides were cleaned of the pDb12H and St2-80 probes.
The probe pDb12H labeled 14 chromosomes of the Jvs genome with green color on both arms, whereas the probe St2-80 gave red-colored signals at distal ends of 14 chromosomes, each of the Jvs and Jr genomes, and in the interstitial regions of 14 short chromosomes of the St genome (Figure 1).
All ten accessions of IWG had the same FISH pattern from the two probes pDb12H and St2-80, showing their common genome constitution JrJrJvsJvsStSt (left side of Figure 2). However, the multiplex oligonucleotide FISH patterns revealed variable signals on some chromosomes of the Jvs and Jr genomes among accessions, or even within an accession (right side of Figure 1 and Figure 2).

2.2. OligoFISH of Three Tetraploid Species of Thinopyrum Genus

Both Th. junceiforme and Th. sartorii had the JvsJvsJrJr genome constitution, as shown in Figure 3 and Figure 4, respectively. Variation in FISH signals between homologous chromosomes could be detected. On the other hand, Th. scirpeum was found to be an autotetraploid having the JrJrJrJr genome composition (Figure 5). The former two species differ from Th. intermedium by lacking the St genome.

2.3. OligoFISH of Four Diploid Species That Were Implicated as Progenitors of IWG

Three diploid species, Th. elongatum, Th. bessarabicum, and Ps. sspicata, were studied using the two probes pDb12H and St2-80 and multiplex oligos pSc119.2-1, (GAA)10 (green), AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6 (red) (Figure 6). None of them showed strong green signals from pDb12H, indicating these species could not be the donor of the Jvs genome in IWG. Some chromosomes of Th. elongatum had very faint green signals in the interstitial regions, whereas no such signals were detected in Th. bessarabicum (Figure 6A–D). Weak green signals of the pDb12H probe were detected at the centromere region of eight chromosomes in Da. Villosum probed by pDb12H and St2-80 (Supplementary Figure S1).
Interestingly, the red signal of St2-80 occurring in Th. elongatum, Th. bessarabicum, and Ps. spicata differed in its distribution on chromosomes. This red color was intense and located on distal ends of chromosomes in the two diploid Thinopyrum species. It was mostly spread out in the interstitial regions of Pseudorogneria chromosomes. There were more chromosomes with the red hybridization signal in Th. bessarabicum (Figure 7) than Th. elongatum (Figure 6).

2.4. In Situ Hybridization of Th. intermedium Using Genomic DNA of Th. elongatum and Th. bessarabicum, and Oligo Probes pDb12H and St2-80

When Th. intermedium was probed with genomic DNA of Th. elongatum and Th. bessarabicum, and the two oligos pDb12H and St2-80, it was found that the genomic DNA of both diploid Thinopyrum species hybridized the same 14 chromosomes (Figure 7A,B). This result indicated that IWG had only one J genome. On the other hand, the oligo probes pDb12H and St2-80 hybridized the other 28 chromosomes, 14 each of the Jvs and St genome, respectively (Figure 7C).

2.5. In Situ Hybridization of Th. intermedium Using Genomic DNA of Dasypyrum villosum and Oligo Probes pDb12H and St2-80

Genomic DNA of Da. villosum and oligo probes pDb12H and St2-80 were used to hybridize the same chromosomes of IWG in a root-tip cell (Figure 8). Both V genomic DNA (Figure 8A) and pDb12H (Figure 8B) hybridized the same 14 chromosomes in Th. intermedium. The St2-80 probe hybridized all 42 chromosomes, but at different sites and at a different intensity. Chromosomes showing green signals of pDb12H had the red signals from St2-80 at telomeric ends. Intense red signals were on 14 short chromosomes, and another 14 chromosomes had red signals at the ends of chromosome arms that embraced bluish interstitial segments.

3. Discussion

3.1. Prior Studies on Thinopyrum intermedium and Related Species

The genome constitution of IWG had been investigated by many research groups and its genome symbol changed over the years, as described by Wang and Lu (2014) [34]. The genome constitution of IWG had been designated as E1E2X [12]. Using the methods of chromosome karyotyping, Giemsa C-banding, and meiotic pairing in hybrids, Liu and Wang [35] concluded that the X genome in Th. intermedium is the S genome from an unspecified Pseudoroegneri pecies. Three tetraploid species, Elytrigia caespitosa, Lophopyrum nodosum, Pseudoroegneria geniculata ssp. scythica, were determined to have the JeS genome constitution in the same study. Then, the symbol S was changed to St in 1995 [13]. The karyotype of Th. intermedium revealed two sets of seven chromosomes that were longer than the third set of seven chromosomes, which was the St genome. When Th. intermedium × Th. bessarabium hybrid was analyzed, seven long chromosomes were attributed to Th. bessarabium (Jb genome); 14 intermediate (Je) and seven short chromosomes (St) were from Th. Intermedium. It was shown in this study that when Jb was present with St, the ratio between the longest and shortest chromosome was about 2.3. The ratio was around 1.8 when Je and St were present in combination. This ratio was 1,3 within the Jb genome.
In 1992, Liu and Wang [36] had given the genome symbol JbJe to both Th. junceiforme and Th. sartorri, but noted variations in the satellite number and size as well as C-banding.
A few years earlier, Pienaar et al. [37] studied the genome relationships in Thinopyrum species. Genome constitution was determined in Th. scirpeum as JeJe, Th. distichum JdJd, Th. junceiforme J1J2.
Using in situ hybridization and molecular markers, Wang and Zhang [14] first used the St genomic DNA in a GISH study of two translocation lines involving Th. intermedium that conferred the resistance to either wheat streak mosaic virus or barley yellow dwarf virus. The presence of St chromatin in these two translocation lines CI17766 and TC14 was substantiated also by St- and E-specific RAPD cloned marker OPB08525 and OPC03340, respectively.
In Canada, Chen et al. (1998) [15] analyzed both Th. intermedium and Th. ponticum using the genomic DNA from Th. elongatum (Host) D.R. Dewey (genome E, 2n = 14), Th. bessarabicum (Savul. & Rayss) Á. Löve (genome J, 2n = 14), and Ps. strigosa (M. Bieb.) Á. Löve (genome St, 2n = 14). They gave Th. intermedium the genome designation JJsS, where J was related to the E genome of Th. elongatum and the J genome of Th. bessarabicum, the S genome was homologous to the S genome of Ps. strigosa, while the Js genome referred to modified J- or E-type chromosomes distinguished by the presence of S genome-specific sequences close to the centromere. However, the Js genome was not composed of fourteen, but only nine to eleven chromosomes.
Later, Kishii et al. (2005) [16] showed that Th. intermedium contains three kinds of genomes: St, E/J, and the third genome might be close to the V genome. However, PCR analysis disconfirmed the presence of the present-day V genome in Th. intermedium, but showed some similarity in the R genome. Thus, the genomic formula of Th. intermedium was tentatively re-designated as StJs(V-J-R)s. Then in 2011, the study of Mahelka et al. [17] further complicated the genome constitution of Th. intermedium by GISH and nuclear GBSSI sequences suggesting that present-day genera Pseudoroegneria, Dasypyrum, Taeniatherum, Aegilops, and Thinopyrum were involved in the evolution of IWG. The DNA of the former two genera consistently hybridized to two genomes of Th. intermedium, but the third genome could be hybridized by those of the other three genera.

3.2. Current Studies on Thinopyrum intermedium and Related Species

The ten accessions of IWG studied here had the same genome constitution JrJrJvsJvsStSt, indicating that IWG populations collected from a wide range of regions (Table 1) had the same evolutionary end product even though it might have gone through different pathways, i.e., different diploid Pseudoroegneria species such as P. libanotica, P. stipieforlia, or P. strigosa could be hybridized by different tetraploid Thinopyrum species such as Th. junceiforme or Th. sartorii. The multiplex oligonucleotide FISH patterns of these IWG accessions, however, revealed variability among and within accessions. These variations could be attributed to the outcrossing nature of this species [38] and multiple hybridization from different sources [17].
Understanding the relationships among J, St, and ABD of wheat, Wang’s laboratory [14,39,40] first used the genomic DNA of St as a probe for the GISH study of wheat hybrids with Th. ponticum and Th. intermedium. The use of St genomic DNA as a GISH probe for the detection of J chromosomes or chromosome fragments in hybrid derivatives of wheat × Thinopyrum intermedium was endorsed as a “landmark approach’ for tracing the introgression of J chromatin into wheat [41]. However, our findings of St2-80 hybridization signals at distal ends of chromosomes in Thinopyrum species (Figure 1, Figure 2, Figure 3, Figure 4, Figure 5 and Figure 6) might set the limitation of this approach of GISH detection for useful alien genes. If the genes of interest were located in the interstitial region of J chromosomes, St genomic probe would likely fail to detect the introgression of alien chromatin.
Because IWG had been proposed to have a haplome formula of JeJeSt [13], naturally occurring tetraploid Thinopyrum species having the genome composition JbJb, JbJe or JeJe would be a potential progenitor of IWG. Indeed, tetraploid Th. junceiforme and Th. sartorii previously shown to have the haplome composition JbJe [36] were found to have the JvsJr portion of JvsJrSt in IWG (Figure 3 and Figure 4). Thus, these two tetraploid Thinopyrum species are now the candidates for a progenitor of IWG. One the other hand, Th. scirpeum that is JrJr (Figure 5) could be excluded from the candidate progenitors of Th. intermedium.
One easy way to ascertain the presence of the St genome along with the J genome is to determine the chromosome length ratio between the longest and shortest chromosomes in the studied species. The ratio will be greater than 2.3 when Jb and St are present; about 1.8 when Je and St are present; and about 1.3 when St is absent [35]. The ratio around 2.3 was observed in karyotypes of Th. intermedium (Figure 1 and Figure 2) where St was present. The ratio around 1.3 was found in Th. junceiforme, Th. sartorii, and Th. scirpeum (Figure 3, Figure 4 and Figure 5), indicating the absence of St. The autotetraploidy of Th. scirpeum [37] is now confirmed by its JrJrJrJr genome composition.
Reporting the development of pDb12H, Yang et al. stated that this FISH probe detected all chromosomes of Da. breviaristatum (see Figure 6 of [21]), but the FISH signal was not detectable in Da. villosum chromosomes in diploid accessions [21]. However, they did not provide the photographic evidence for the presence or absence of pDb12H in Da. villosum. Our Figure S1 clearly shows that pDb12H could only weakly hybridize the pericentromeric regions of eight chromosomes of Da. villosum. This result supports the conclusion that Da. villoum and Da. breviaristatum are distinct, deserving the designation of genome symbols Vv and Vb, respectively [42,43]. It also substantiates the conclusion that the present-day V genome of Da. villosum is not the Jvs genome [23].
Most importantly, the observations that genomic DNA of Th. elongatum or Th. bessarabicum hybridized only to the same 14 chromosomes in IWG (Figure 7A,B) indicate that IWG had only one J genome. This observation is unique and different because the same root-tip cell was used in sequential GISH experiments. All other studies mentioned earlier had different root-tip cells used for GISH using different probes. Our finding in this study supports that reported for Th. junceiforme [44]. Furthermore, the Jvs genome could be hybridized by either genomic DNA of Da. villosum or oligo probe pDb12H (Figure 8), indicating that Jvs was likely a progenitor of Da. breviaristatum and Da. villosum. Based on Figure S1, it can be inferred that the Vv genome of the latter had a much lower copy number of the pDb12H sequence than Vb of Da. breviaristatum. When more evidence becomes available, the genome symbol Jvs might be changed to Vb.

3.3. Future Studies on Thinopyrum intermedium and Related Species Needed

The recent development of the precise identification of Th. intermedium chromosome compliment [45] and chromosome-specific bulked oligonucleotides for identifying E-genome chromosomes in both Th. bessarabicum and Th. elongatum [46] would be useful in future studies on all Thinopyrum species and wheat × Thinopyrum hybrid derivatives.
In addition, a polyhaploid of Th. intermedium (2n = 3x = 21, JvsJrSt) and the hybrid between Th. intermedium and different Pseudoroegneria species (2n = 4x = 28, JvsJrStSt) should be made. Then, oFISH using pDb12H [21,22], St2-80 [20], and pMD232-500 [18] should be carried out on both root-tip cells and pollen mother cells of these plants. The results from oFISH of pollen mother cells would reveal whether the two genomes Jvs and Jr are capable of high pairing. If the two genomes can pair to form four or more bivalents, their genome symbols would stay. If the two genomes cannot form any bivalent, the Jvs might have to be changed to V.

4. Materials and Methods

4.1. Plant Materials

Thinopyrum intermedium (2n = 6x = 42), Th. junceiforme (2n = 4x = 28), Th. sartorii (2n = 4x = 28), Th. scirpeum (2n = 4x = 28), Th. elongatum (2n = 2x = 14, EeEe), Th. bessarabicum (2n = 2x = 14, JJ or EbEb), Pseudowroegneria spicata (2n = 2x = 14, StSt) having the PI numbers were kindly provided by the Germplasm Resource Information Network (GRIN) of United States Department of Agriculture (Table 1). Dasypyrum villosum (2n = 2x = 14, VV) were obtained from Prof. Xingfeng Li, College of Agronomy, Shandong Agricultural University. All plant materials were maintained through selfing at the Tai’an Subcenter of the National Wheat Improvement Center, Tai’an, China.

4.2. DNA Extraction and Probe Preparation

The CTAB method was used to extract total genomic DNA from Th. elongatum, Th. bessarabicum, Ps. strigose, Da. villosum. Two oligonucleotide probes used for the FISH studies were St2-80 [20] and pDb12H [21,22]. pDbH12 could serve as a cytogenetic marker for tracing chromatin from the Vb genome in wheat–alien introgression lines. St2-80 is a potential and useful FISH marker that can be used to distinguish St and other genomes in Triticeae. Fluorescent signals of Th. elongatum (Ee = Je), Th. bessarabicum (Eb = Jb), and Ps. strigose (St) genomic DNA as well as St2-80 were labeled with Texas-red-5-dCTP, while pDb12H and Da. villosum (V) genomic DNA were labeled with fluorescein-12-dUTP using the nick translation method. Oligonucleotides (synthesized by Sangon Biotech, Shanghai, China) pSc119.2-1 and (GAA)10 were labeled with 5′-FAM (5-carboxyfluorescein), while AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6 were labeled with 5′-TAMRA (5-carboxytetramethylrhodamine) as described by Du et al. 2017 [47].

4.3. Chromosome Preparation and GISH, FISH Protocol

Fresh root-tip cells (RTCs) collected from germinating seeds were treated with 1.0 MPa nitrous oxide (N2O) for 2 h [48] and then immersed in 90% glacial acetic acid. RTC slides were prepared according to the procedure used in Prof. Han’s laboratory [49]. Once spread chromosomes were found under the phase-contrast microscope, the slides were put in liquid nitrogen for freezing and the coverslips were removed. Then slides were dehydrated in ethanol and dried at room temperature. After the above steps, the slides were subjected to FISH. The procedures of GISH, FISH, and signal detection were conducted according to the method of Du et al. (2017) [47] and He et al. (2017) [50]. The probe labelling, denaturation, image capture, and data processing were described in Cui et al. (2019) [51]. Images were collected using a NIKON eclipse Ni-U fluorescence microscope; the images were processed using NIS-Elements BR 4.00.12 software.

5. Conclusions

In this study, we found that using two oligonucleotides pDb12H and St2-80 as probes in FISH was sufficient to distinguish the three genomes Jvs, Jr, and St in intermediate wheatgrass. In addition, variations in the outcrossing Th. intermedium were visible in multiplex oFISH both among and within accessions. Th. junceiforme and Th. sartorii were probable progenitors of IWG. Jr is related to either Je of Th. elongatum or Jb of Th. bessarabicum. Finally, the Jvs genome could be the progenitor of present-day Vb and Vv; thus, this genome symbol might be changed to V when enough evidence becomes available.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/plants12213705/s1, Figure S1. Oligonucleotide florescence in situ hybridization of chromosomes in Dasypyrum villosum. Probes are pDb12H (green) and St2-80 (red).

Author Contributions

Conceptualization, R.R.-C.W. and X.L.; methodology, F.Q. and P.X.; validation, F.Q.; data curation, F.Q., S.L. and P.X.; formal analysis, Y.B.; investigation, F.Q., S.L. and P.X.; writing—original draft preparation, R.R.-C.W. and X.L.; writing—review and editing, R.R.-C.W. and X.L.; supervision, X.L.; project administration, X.L.; funding acquisition, R.R.-C.W. and X.L. All authors have read and agreed to the published version of the manuscript.

Funding

The National Natural Science Foundation of 408 China (31671675) and USDA CRIS 2080-21000-018-000D.

Data Availability Statement

All figures in this article are available for use without restrictions.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Kumar, A.; Choudhary, A.; Kaur, H.; Mehta, S. A walk towards Wild grasses to unlock the clandestine of gene pools for wheat improvement: A review. Plant Stress 2022, 3, 100048. [Google Scholar] [CrossRef]
  2. Altendorf, K.R.; DeHaan, L.R.; Heineck, G.C.; Zhang, X.; Anderson, J.A. Floret site utilization and reproductive tiller number are primary components of grain yield in intermediate wheatgrass spaced plants. Crop Sci. 2021, 61, 1073–1088. [Google Scholar] [CrossRef]
  3. Mujeeb-Kazi, A.; Kazi, A.G.; Dundas, I.; Rasheed, A.; Ogbonnaya, F.; Kishii, M.; Bonnett, D.; Wang, R.R.C.; Xu, S.; Chen, P.; et al. Genetic diversity for wheat improvement as a conduit to food security. In Advances in Agronomy; Sparks, D.L., Ed.; Academic Press: Cambridge, MA, USA, 2013; Volume 122, pp. 179–258. [Google Scholar]
  4. Li, W.; Danilova, T.; Rouse, M.N.; Bowden, R.L.; Friebe, B.; Gill, B.S.; Pumphrey, M.O. Development and characterization of a compensating wheat-Thinopyrum intermedium Robertsonian translocation with Sr44 resistance to stem rust (Ug99). Theor. Appl. Genet. 2013, 126, 1167–1177. [Google Scholar] [CrossRef] [PubMed]
  5. Cui, L.; Ren, Y.K.; Bao, Y.G.; Nan, H.; Tang, Z.H.; Guo, Q.; Niu, Y.Q.; Yan, W.Z.; Sun, Y.; Li, H.J. Assessment of Resistance to Cereal Cyst Nematode, Stripe Rust, and Powdery Mildew in Wheat-Thinopyrum intermedium Derivatives and Their Chromosome Composition. Plant Disease 2021, 105, 2898–2906. [Google Scholar] [CrossRef]
  6. Ivanova, Y.N.; Rosenfread, K.K.; Stasyuk, A.I.; Skolotneva, E.; Silkova, O.G. Raise and characterization of a bread wheat hybrid line (Tulaykovskaya 10 × Saratovskaya 29) with chromosome 6Agi2 introgressed from Thinopyrum intermedium. Vavilov J. Genet. Breed. 2021, 25, 701–712. [Google Scholar] [CrossRef] [PubMed]
  7. Zhang, X.F.; DeHaan, L.R.; Higgins, L.A.; Markowski, T.W.; Wyse, D.L.; Anderson, J.A. New insights into high-molecular-weight glutenin subunits and subgenomes of the perennial crop Thinopyrum intermedium (Triticeae). J. Cereal Sci. 2014, 59, 203–210. [Google Scholar] [CrossRef]
  8. Marti, A.; Bock, J.; Pagani, M.; Ismail, B.; Seetharaman, K. Structural characterization of proteins in wheat flour doughs enriched with intermediate wheatgrass (Thinopyrum intermedium) flour. Food Chem. 2016, 194, 994–1002. [Google Scholar] [CrossRef]
  9. Crain, J.; Larson, S.R.; Dorn, K.M.; Dehaan, L.; Poland, J. Genetic architecture and QTL selection response for Kernza perennial grain domestication traits. Theor. Appl. Genet. 2022, 135, 2769–2784. [Google Scholar] [CrossRef]
  10. Locatelli, A.; Gutierrez, L.; Picasso Risso, V.D. Vernalization requirements of Kernza intermediate wheatgrass. Crop Sci. 2022, 62, 524–535. [Google Scholar] [CrossRef]
  11. de Oliveira, G.; Brunsell, N.A.; Sutherlin, C.E.; Crews, T.E.; DeHaan, L.R. Energy, water and carbon exchange over a perennial Kernza wheatgrass crop. Agric. For. Meteorol. 2018, 249, 120–137. [Google Scholar] [CrossRef]
  12. Dewey, D.R. The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In Gene Manipulation in Plant Improvement; Gustafson, J.P., Ed.; Plenum Publishing Corporation: New York, NY, USA, 1984; pp. 209–279. [Google Scholar]
  13. Wang, R.R.-C.; von Bothmer, R.; Dvorák, J.; Fedak, G.; Linde-Laursen, I.; Muramatsu, M. Genome symbols in the Triticeae (Poaceae). In Proceedings of the 2nd International Triticeae Symposium, Logan, UT, USA, 20–24 June 1994; Wang, R.R.-C., Jensen, K.B., Jaussi, C., Eds.; Utah State University Publication Design and Production: Logan, UT, USA, 1995; pp. 29–34. [Google Scholar]
  14. Wang, R.R.-C.; Zhang, X.-Y. Characterization of the translocated chromosome using fluorescence in situ hybridization and random amplified polymorphic DNA on two Triticum aestivum-Thinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus. Chromosome Res. 1996, 4, 583–587. [Google Scholar] [CrossRef] [PubMed]
  15. Chen, Q.; Conner, R.L.; Laroche, A.; Thomas, J.B. Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization. Genome 1998, 41, 580–586. [Google Scholar] [CrossRef] [PubMed]
  16. Kishii, M.; Wang, R.R.-C.; Tsujimoto, H. GISH analysis revealed new aspect of genomic constitution of Thinopyrum intermedium. Czechoslov. J. Genet. Plant Breed. 2005, 41, 92–95. [Google Scholar] [CrossRef]
  17. Mahelka, V.; Kopecký, D.; Paštová, L. On the genome constitution and evolution of intermediate wheatgrass (Thinopyrum intermedium: Poaceae, Triticeae). BMC Evol. Biol. 2011, 11, 127. [Google Scholar] [CrossRef] [PubMed]
  18. Tang, Z.X.; Yang, Z.J.; Fu, S.L.; Yang, M.Y.; Li, G.R.; Zhang, H.Q.; Tan, F.Q.; Ren, Z. A new long terminal repeat (LTR) sequence allows the identify J genome from Js and St genomes of Thinopyrum intermedium. J. Appl. Genet. 2011, 52, 31–33. [Google Scholar] [CrossRef] [PubMed]
  19. Deng, C.L.; Bai, L.L.; Fu, S.L.; Yin, W.B.; Zhang, Y.X.; Chen, Y.H.; Wang, R.R.-C.; Zhang, X.Q.; Han, F.P.; Hu, Z.M. Microdissection and chromosome painting of the alien chromosome in an addtition line of wheat-Thinopyrum intermedium. PLoS ONE 2013, 8, e72564. [Google Scholar] [CrossRef]
  20. Wang, L.; Shi, Q.; Su, H.; Wang, Y.; Sha, L.; Fan, X.; Kang, H.; Zhang, H.; Zhou, Y. St2-80: A new FISH marker for St genome and genome analysis in Triticeae. Genome 2017, 60, 553–563. [Google Scholar] [CrossRef]
  21. Yang, Z.-J.; Liu, C.; Feng, J.; Li, G.-R.; Zhou, J.-P.; Deng, K.-J.; Ren, Z.-L. Studies on genome relationship and species-specific PCR marker for Dasypyrum breviaristatumin Triticeae. Hereditas 2006, 143, 47–54. [Google Scholar] [CrossRef]
  22. Liu, C.; Yang, Z.J.; Jia, J.Q.; Li, G.R.; Zhou, J.P.; Ren, Z.L. Genomic distribution of a long end repeat (LTR) Sabrina-like retrotransposon in Triticeae species. Cereal Res. Commun. 2009, 37, 363–372. [Google Scholar] [CrossRef]
  23. Wang, R.R.-C.; Larson, S.R.; Jensen, K.B.; Bushman, B.S.; DeHaan, L.R.; Wang, S.; Yan, X. Genome evolution of intermediate wheatgrass as revealed by EST-SSR markers developed from its three progenitor diploid species. Genome 2015, 58, 63–70. [Google Scholar] [CrossRef]
  24. Cseh, A.; Yang, C.Y.; Hubbart-Edwards, S.; Scholefield, D.; Ashling, S.S.; Burridgem, A.J.; Wilkinson, P.A.; King, I.P.; King, J.; Grewal, S. Development and validation of an exome-based SNP marker set for identification of the St, Jr and Jvs genomes of Thinopyrym intermedium in a wheat background. Theor. Appl. Genet. 2019, 132, 1555–1570. [Google Scholar] [CrossRef] [PubMed]
  25. Divashuk, M.G.; Karlov, G.I.; Kroupin, P.Y. Copy Number Variation of Transposable Elements in Thinopyrum intermedium and Its Diploid Relative Species. Plants 2019, 9, 15. [Google Scholar] [CrossRef] [PubMed]
  26. Li, X.-M.; Lee, B.S.; Mammadov, A.C.; Koo, B.-C.; Mott, I.W.; Wang, R.R.-C. CAPS markers specific to Eb, Ee and R genomes in the tribe Triticeae. Genome 2007, 50, 400–411. [Google Scholar] [CrossRef] [PubMed]
  27. Kantarski, T.; Larson, S.; Zhang, X.; DeHaan, L.; Borevitz, J.; Anderson, J.; Poland, J. Development of the first consensus genetic map of intermediate wheatgrass (Thinopyrum intermedium) using genotyping-by-sequencing. Theor. Appl. Genet. 2017, 130, 137–150. [Google Scholar] [CrossRef] [PubMed]
  28. Larson, S.; DeHaan, L.; Poland, J.; Zhang, X.; Dorn, K.; Kantarski, T.; Anderson, J.; Schmutz, J.; Grimwood, J.; Jenkins, J.; et al. Genome mapping of quantitative trait loci (QTL) controlling domestication traits of intermediate wheatgrass (Thinopyrum intermedium). Theor. Appl. Genet. 2019, 132, 2325–2351. [Google Scholar] [CrossRef] [PubMed]
  29. DeHaan, L.; Christians, M.; Crain, J.; Poland, J. Development and evolution of an intermediate wheatgrass domestication program. Sustainability 2018, 10, 1499. [Google Scholar] [CrossRef]
  30. Qiao, L.; Liu, S.; Li, J.; Li, S.; Yu, Z.; Liu, C.; Li, X.; Liu, J.; Ren, Y.; Zhang, P.; et al. Development of Sequence-Tagged Site Marker Set for Identification of J, JS, and St Sub-genomes of Thinopyrum intermedium in Wheat Background. Front. Plant Sci. 2021, 12, 685216. [Google Scholar] [CrossRef]
  31. Gao, L.; Ma, Q.; Liu, Y.; Xin, Z.; Zhang, Z. Molecular characterization of the genomic region harboring the BYDV-resistance gene Bdv2 in wheat. J. Appl. Genet. 2009, 50, 89–98. [Google Scholar] [CrossRef]
  32. Ceoloni, C.; Kuzmanovi’c, L.; Gennaro, A.; Forte, P.; Giorgi, D.; Grossi, M.R.; Bitti, A. Genomes, Chromosomes and Genes of the Wheatgrass Genus Thinopyrum: The Value of their Transfer into Wheat for Gains in Cytogenomic Knowledge and Sustainable Breeding. In Genomics of Plant Genetic Resources; Tuberosa, R., Graner, A., Frison, E., Eds.; Springer: Dordrecht, The Netherlands, 2014; Chapter 14; pp. 333–358. [Google Scholar]
  33. Guo, X.; Huang, Y.; Wang, J.; Fu, S.; Wang, C.; Wang, M.; Zhou, C.; Hu, X.; Wang, T.; Yang, W.; et al. Development and cytological characterization of wheat– Thinopyrum intermedium translocation lines with novel stripe rust resistance gene. Front. Plant Sci. 2023, 14, 1135321. [Google Scholar] [CrossRef]
  34. Wang, R.R.-C.; Lu, B.R. Biosystematics and evolutionary relationships of perennial Triticeae species revealed by genomic analyses. J. Syst. Evol. 2014, 52, 697–705. [Google Scholar] [CrossRef]
  35. Liu, Z.W.; Wang, R.R.-C. Genome analysis of Elytrigia caespitosa, Lophopyrum nodosum, Pseudoroegneria geniculate ssp. scythica, and Thinopyrum intermedium. Genome 1993, 36, 102–111. [Google Scholar] [CrossRef] [PubMed]
  36. Liu, Z.-W.; Wang, R.R.-C. Genome analysis of Thinopyrum junceiforme and T. sartorii. Genome 1992, 35, 758–764. [Google Scholar] [CrossRef]
  37. Pienaar, R.d.V.; Littlejohn, G.M.; Sears, E.R. Genomic relationships in Thinopyrum. S. Afr. J. Bot. 1988, 54, 541–550. [Google Scholar] [CrossRef]
  38. Jensen, K.B.; Zhang, Y.F.; Dewey, D.R. Mode of pollination of perennial species of the Triticeae in relation to genomically defined genera. Can. J. Plant Sci. 1990, 70, 215–225. [Google Scholar] [CrossRef]
  39. Zhang, X.Y.; Dong, Y.C.; Wang, R.R.-C. Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum ×Thinopyrum ponticum by in situ hybridization, isozyme analysis, and RAPD. Genome 1996, 39, 1062–1071. [Google Scholar] [CrossRef] [PubMed]
  40. Zhang, X.-Y.; Koul, A.; Petroski, R.; Ouellet, T.; Fedak, G.; Dong, Y.S.; Wang, R.R.-C. Molecular verification and characterization of BYDV-resistant germplasms derived from hybrids of wheat with Thinopyrum ponticum and Th. intermedium. Theor. Appl. Genet. 1996, 93, 1033–1039. [Google Scholar] [CrossRef] [PubMed]
  41. Chen, Q. Detection of alien chromatin introgression from Thinopyrum into wheat using S genomic DNA as a probe—A landmark approach for Thinopyrum genome research. Cytogenet. Genome Res. 2005, 109, 350–359. [Google Scholar] [CrossRef] [PubMed]
  42. Ohta, S.; Morishita, M. Genome relationship in the genus of Dasypyrum (Gramineae). Hereditas 2001, 135, 101–110. [Google Scholar] [CrossRef]
  43. Li, G.; Gao, D.; Zhang, H.; Li, J.; Wang, H.; La, S.; Ma, J.; Yang, Z. Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species. Mol. Cytogenet. 2016, 9, 6. [Google Scholar] [CrossRef]
  44. Li, W.; Zhang, Q.; Wang, S.; Langham, M.A.; Singh, D.; Bowden, R.L.; Xu, S.S. Development and characterization of wheat-sea wheatgrass (Thinopyrum junceiforme) amphiploids for biotic stress resistance and abiotic stress tolerance. Theor. Appl. Genet. 2019, 132, 163–175. [Google Scholar] [CrossRef]
  45. Yu, Z.; Wang, H.; Yang, E.; Li, G.; Yang, Z. Precise Identification of Chromosome Constitution and Rearrangements in Wheat–Thinopyrum intermedium Derivatives by ND-FISH and Oligo-FISH Painting. Plants 2022, 11, 2109. [Google Scholar] [CrossRef] [PubMed]
  46. Chen, C.; Han, Y.S.; Xiao, H.; Zou, B.C.; Wu, D.D.; Sha, L.A.; Yang, C.R.; Liu, S.Q.; Cheng, Y.R.; Wang, Y.; et al. Chromosome-specific painting in Thinopyrum species using bulked oligonucleotides. Theor. Appl. Genet. 2023, 136, 8. [Google Scholar] [CrossRef] [PubMed]
  47. Du, P.; Zhuang, L.; Wang, Y.; Yuan, L.; Wang, Q.; Wang, D.; Dawadondup, X.; Tan, L.; Shen, J.; Xu, H.; et al. Development of oligonucleotides and multiplex probes for quick and accurate identification of wheat and Thinopyrum bessarabicum chromosomes. Genome 2017, 60, 93–103. [Google Scholar] [CrossRef] [PubMed]
  48. Kato, A. Air drying method using nitrous oxide for chromosome counting in maize. Biotech. Histochem. 1999, 74, 160–166. [Google Scholar] [CrossRef] [PubMed]
  49. Han, F.P.; Liu, B.; Fedak, G.; Liu, Z.H. Genomic constitution and variation in five partial amphiploids of wheat–Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. Theor. Appl. Genet. 2004, 109, 1070–1076. [Google Scholar] [CrossRef] [PubMed]
  50. He, F.; Xing, P.; Bao, Y.; Ren, M.; Liu, S.; Wang, Y.; Li, X.; Wang, H. Chromosome pairing in hybrid progeny between Triticum aestivum and Elytrigia elongata. Front. Plant Sci. 2017, 8, 2161. [Google Scholar] [CrossRef] [PubMed]
  51. Cui, Y.; Xing, P.; Qi, X.; Bao, Y.; Wang, H.; Wang, R.R.-C.; Li, X.F. Characterization of chromosome constitution in three wheat—Thinopyrum intermedium amphiploids revealed frequent rearrangement of alien and wheat chromosomes. BMC Plant Biol. 2021, 21, 129. [Google Scholar] [CrossRef]
Plants 12 03705 g001
Figure 1. FISH characterized chromosomes (top: (A,B)) and karyotypes (bottom) of Thinopyrum intermedium PI 325190. (Bottom) left side: probed with pDb12H (green) and St2-80 (red); right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10 (green), AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6 (red), Scale bar: 10 μm.
Figure 1. FISH characterized chromosomes (top: (A,B)) and karyotypes (bottom) of Thinopyrum intermedium PI 325190. (Bottom) left side: probed with pDb12H (green) and St2-80 (red); right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10 (green), AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6 (red), Scale bar: 10 μm.
Plants 12 03705 g001
Plants 12 03705 g002
Figure 2. Karyotypes of ten accessions of Thinopyrum intermedium (top to bottom) PI 206259, PI 210990, PI 228386, PI297876, PI 325190, PI 440096, PI 249146, PI 109219, PI 634290, and PI 401204. Left side: probed with pDb12H (green) and St2-80 (red). Right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10 (green), AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6 (red).
Figure 2. Karyotypes of ten accessions of Thinopyrum intermedium (top to bottom) PI 206259, PI 210990, PI 228386, PI297876, PI 325190, PI 440096, PI 249146, PI 109219, PI 634290, and PI 401204. Left side: probed with pDb12H (green) and St2-80 (red). Right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10 (green), AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6 (red).
Plants 12 03705 g002
Plants 12 03705 g003
Figure 3. FISH characterized chromosomes and karyotypes of Thinopyrum junceiforme PI 414667. Left side: probed with pDb12H (green) and St2-80 (red). Right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6. Variations in FISH signals between homologous chromosomes are detected (in rectangular boxes).
Figure 3. FISH characterized chromosomes and karyotypes of Thinopyrum junceiforme PI 414667. Left side: probed with pDb12H (green) and St2-80 (red). Right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6. Variations in FISH signals between homologous chromosomes are detected (in rectangular boxes).
Plants 12 03705 g003
Plants 12 03705 g004
Figure 4. FISH characterized chromosomes (top) and karyotypes (bottom) of Thinopyrum sartorii PI 531745. Left side: probed with pDb12H (green) and St2-80 (red). Right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6.
Figure 4. FISH characterized chromosomes (top) and karyotypes (bottom) of Thinopyrum sartorii PI 531745. Left side: probed with pDb12H (green) and St2-80 (red). Right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6.
Plants 12 03705 g004
Plants 12 03705 g005
Figure 5. FISH characterized chromosomes (top) and karyotypes (bottom) of Thinopyrum scirpeum PI 531750. Left side: probed with pDb12H (green) and St2-80 (red). Right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6.
Figure 5. FISH characterized chromosomes (top) and karyotypes (bottom) of Thinopyrum scirpeum PI 531750. Left side: probed with pDb12H (green) and St2-80 (red). Right side: probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6.
Plants 12 03705 g005
Plants 12 03705 g006
Figure 6. Oligonucleotide FISH of Thinopyrum elongatum (A), Th. bessarabicum (C), and Pseudoroegneria strigose (E), probed with pDb12H (green) and St2-80 (red). Oligonucleotide FISH of Thinopyrum elongatum (B), Th. bessarabicum (D), and Pseudoroegneria strigose (F), probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10 (green), AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6 (red).
Figure 6. Oligonucleotide FISH of Thinopyrum elongatum (A), Th. bessarabicum (C), and Pseudoroegneria strigose (E), probed with pDb12H (green) and St2-80 (red). Oligonucleotide FISH of Thinopyrum elongatum (B), Th. bessarabicum (D), and Pseudoroegneria strigose (F), probed with multiplex oligonucleotides, including pSc119.2-1, (GAA)10 (green), AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6 (red).
Plants 12 03705 g006
Plants 12 03705 g007
Figure 7. Thinopyrum intermedium probed with genomic DNA of Th. elongatum (A), Th. bessarabicum (B), and oligo pDb12H and St2-80 (C). All three experiments were conducted with the same root-tip cell, Scale bar: 10 μm.
Figure 7. Thinopyrum intermedium probed with genomic DNA of Th. elongatum (A), Th. bessarabicum (B), and oligo pDb12H and St2-80 (C). All three experiments were conducted with the same root-tip cell, Scale bar: 10 μm.
Plants 12 03705 g007
Plants 12 03705 g008
Figure 8. Chromosomes of Thinopyrum intermedium probed with (A) genomic DNA of Dasypyrum villosum and (B) oligos pDb12H (green) and St2-80 (red).
Figure 8. Chromosomes of Thinopyrum intermedium probed with (A) genomic DNA of Dasypyrum villosum and (B) oligos pDb12H (green) and St2-80 (red).
Plants 12 03705 g008
Table 1. Plant materials used in this study.
Table 1. Plant materials used in this study.
SpeciesIDChr NumberOriginNote
Thinopyrum intermedium (Host) Barkworth & D. R. DeweyPI 10921942District of Columbia, United States
PI 20625942Turkey
PI 21099042Afghanistan
PI 22838642Iran
PI 24914642Portugal
PI 29787642Former, Soviet Union
PI 32519042Stavropol, Russian
Federation
PI 40120442Iran
PI 44003642Kazakhstan
PI 63429042Krym, Ukraine
Th. junceiforme (A. & D. Löve) A. LövePI 41466728Greecelisted as Thinopyrum junceum (L.) Á. Löve
Th. sartorii (Bioss. & Heldr.) Á. LövePI 53174528Greece
Th. scirpeum (C. Presl) D. R. DeweyPI 53175028Greece
Th. bessarabicum (Savul. & Rayss) A. LövePI 53171214Estonia
Th. elongatum (Host) D. R. DeweyPI 34006314Turkey
Pseudoroegneria spicata Pursh) Á. LövePI 56386914Oregon, USA
Dasypyrum villosum (L.) Candargy 14 From X-F Li’s collection
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Qi, F.; Liang, S.; Xing, P.; Bao, Y.; Wang, R.R.-C.; Li, X. Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH. Plants 2023, 12, 3705. https://doi.org/10.3390/plants12213705

AMA Style

Qi F, Liang S, Xing P, Bao Y, Wang RR-C, Li X. Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH. Plants. 2023; 12(21):3705. https://doi.org/10.3390/plants12213705

Chicago/Turabian Style

Qi, Fei, Shuang Liang, Piyi Xing, Yinguang Bao, Richard R.-C. Wang, and Xingfeng Li. 2023. "Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH" Plants 12, no. 21: 3705. https://doi.org/10.3390/plants12213705

APA Style

Qi, F., Liang, S., Xing, P., Bao, Y., Wang, R. R.-C., & Li, X. (2023). Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH. Plants, 12(21), 3705. https://doi.org/10.3390/plants12213705

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop