Functional Differences of Grapevine Circular RNA Vv-circPTCD1 in Arabidopsis and Grapevine Callus under Abiotic Stress
Abstract
:1. Introduction
2. Results
2.1. Identification and Verification of Vv-circPTCD1
2.2. Nonconservative Back-Splicing of Vv-circPTCD1 in Plants
2.3. The Expression Analyses of PTCD1 and Vv-circPTCD1 under Abiotic Stress
2.4. Overexpression of Vv-circPTCD1 in Grapevine Callus and Arabidopsis
2.5. Phenotype of OE Lines under Heat Stress
2.6. Phenotype of OE Lines under Salt Stress
2.7. Phenotype of OE Lines under Drought Stress
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Validation of circRNA and RT-qPCR
4.3. Vector Construction
4.4. Arabidopsis Transformation and Verification
4.5. Callus Transformation of ‘Thompson Seedless’ and Treatment
4.6. Statistical Analysis and Gene Structure
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Ren, Y.; Li, J.; Liu, J.; Zhang, Z.; Song, Y.; Fan, D.; Liu, M.; Zhang, L.; Xu, Y.; Guo, D.; et al. Functional Differences of Grapevine Circular RNA Vv-circPTCD1 in Arabidopsis and Grapevine Callus under Abiotic Stress. Plants 2023, 12, 2332. https://doi.org/10.3390/plants12122332
Ren Y, Li J, Liu J, Zhang Z, Song Y, Fan D, Liu M, Zhang L, Xu Y, Guo D, et al. Functional Differences of Grapevine Circular RNA Vv-circPTCD1 in Arabidopsis and Grapevine Callus under Abiotic Stress. Plants. 2023; 12(12):2332. https://doi.org/10.3390/plants12122332
Chicago/Turabian StyleRen, Yi, Junpeng Li, Jingjing Liu, Zhen Zhang, Yue Song, Dongying Fan, Minying Liu, Lipeng Zhang, Yuanyuan Xu, Dinghan Guo, and et al. 2023. "Functional Differences of Grapevine Circular RNA Vv-circPTCD1 in Arabidopsis and Grapevine Callus under Abiotic Stress" Plants 12, no. 12: 2332. https://doi.org/10.3390/plants12122332