Transcriptome and Physiological Analysis of Rootstock Types and Silicon Affecting Cold Tolerance of Cucumber Seedlings
Abstract
:1. Introduction
2. Results
2.1. Physiological Response of Cucumber Seedlings under Cold Stress
2.2. Transcriptome Sequencing Data and Quality
2.3. Statistics of DEGs
2.4. Functional Annotation of DEGs
2.5. KEGG Enrichment Analysis of Silicon-Induced DEGs
2.6. KEGG Enrichment Analysis of Rootstock-Grafted-Induced DEGs
2.7. Screening for Transcription Factors
2.8. Validation of the Transcriptome Data
3. Discussion
3.1. Silicon Could Enhance the Cold-Tolerance of Cucumber Seedlings by Enhancing the Metabolism of Phenylpropanoid
3.2. Rootstock Grafting Could Boost the Scavenging of ROS and the Synthesis of Phytohormones and Maintain the Stability of Membrane Structure to Enhance the Cold-Tolerance of Cucumber Seedlings
3.3. MYB, ERF and WRKY Play Transcription Factor Family Plays a Role in Cucumber Seedlings Response to Cold Stresses
4. Materials and Methods
4.1. Plant Materials and Cold Treatment
4.2. Determination of Physiological Characteristics under Cold Stress
4.3. Preparation and Sequencing of RNA Sequence Libraries
4.4. Data Filtering and Mapping of Reads
4.5. Screening of Differentially Expressed Genes (DEGs)
4.6. Functional Annotation and Enrichment Analysis of DEGs
4.7. Transcription Factor Analysis and Screening
4.8. Quantitative Real-Time PCR (qRT-PCR) Analysis
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compare | Total DEGs | Up | Down |
---|---|---|---|
Z+_vs_Z | 433 | 274 | 159 |
H_vs_Z | 412 | 264 | 148 |
Y_vs_Z | 1217 | 679 | 538 |
Y_vs_H | 305 | 107 | 198 |
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Luan, H.; Niu, C.; Nie, X.; Li, Y.; Wei, M. Transcriptome and Physiological Analysis of Rootstock Types and Silicon Affecting Cold Tolerance of Cucumber Seedlings. Plants 2022, 11, 445. https://doi.org/10.3390/plants11030445
Luan H, Niu C, Nie X, Li Y, Wei M. Transcriptome and Physiological Analysis of Rootstock Types and Silicon Affecting Cold Tolerance of Cucumber Seedlings. Plants. 2022; 11(3):445. https://doi.org/10.3390/plants11030445
Chicago/Turabian StyleLuan, Heng, Chenxu Niu, Xinmiao Nie, Yan Li, and Min Wei. 2022. "Transcriptome and Physiological Analysis of Rootstock Types and Silicon Affecting Cold Tolerance of Cucumber Seedlings" Plants 11, no. 3: 445. https://doi.org/10.3390/plants11030445