The Novel Cucurbitaceae miRNA ClmiR86 Is Involved in Grafting-Enhanced Phosphate Utilization and Phosphate Starvation Tolerance in Watermelon
Abstract
:1. Introduction
2. Results
2.1. ClmiR86 May Enhance PUE of Watermelon
2.2. ClmiR86 Suppresses ClCIPK5, AtCIPK5, and AtCIPK25 at the Post-Transcription Level
2.3. Ectopic ClmiR86 Expression Enhanced Phosphate Starvation Tolerance
2.4. Ectopic ClmiR86 Expression Enhanced Plant Growth under Normal Conditions
3. Discussion
3.1. Squash Grafting Enhanced Watermelon Phosphate Utilization
3.2. ClmiR86 Is Involved in Phosphate Starvation Response and Phosphate Utilization in Watermelon
3.3. CIPK5 Works Downstream of ClmiR86 under Phosphate Starvation and Normal Conditions
4. Materials and Methods
4.1. Plant Materials, Growth Conditions, and Treatments
4.2. Vector Construction
4.3. Arabidopsis Transformation
4.4. Target Gene Prediction
4.5. Phylogenetic Tree Construction
4.6. PUE Measurement
4.7. RNA Isolation, cDNA Synthesis, and qRT-PCR
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Wu, W.; Zhao, H.; Deng, Q.; Yang, H.; Guan, X.; Qi, R.; Shi, P.; Yang, J.; Zhang, M.; Hu, Z. The Novel Cucurbitaceae miRNA ClmiR86 Is Involved in Grafting-Enhanced Phosphate Utilization and Phosphate Starvation Tolerance in Watermelon. Plants 2021, 10, 2133. https://doi.org/10.3390/plants10102133
Wu W, Zhao H, Deng Q, Yang H, Guan X, Qi R, Shi P, Yang J, Zhang M, Hu Z. The Novel Cucurbitaceae miRNA ClmiR86 Is Involved in Grafting-Enhanced Phosphate Utilization and Phosphate Starvation Tolerance in Watermelon. Plants. 2021; 10(10):2133. https://doi.org/10.3390/plants10102133
Chicago/Turabian StyleWu, Weifang, Haoshun Zhao, Qin Deng, Haiyang Yang, Xiaoxiao Guan, Rui Qi, Pibiao Shi, Jinghua Yang, Mingfang Zhang, and Zhongyuan Hu. 2021. "The Novel Cucurbitaceae miRNA ClmiR86 Is Involved in Grafting-Enhanced Phosphate Utilization and Phosphate Starvation Tolerance in Watermelon" Plants 10, no. 10: 2133. https://doi.org/10.3390/plants10102133