Hippeastrum stapfianum (Kraenzl.) R.S.Oliveira & Dutilh (Amaryllidaceae) Ethanol Extract Activity on Acetylcholinesterase and PPAR-α/γ Receptors
Abstract
:1. Introduction
2. Results
2.1. EE Upregulates the PPAR-α and PPAR-γ Receptor
2.2. EE Performs Antioxidant Capacity and Promotes Acetylcholinesterase Inhibition In Vitro
2.3. EE and Fractions Chemical Characterization by GC-MS Analysis and HPLC
3. Discussion
4. Materials and Methods
4.1. Hippeastrum stapfianum Plant Material
4.2. Hippeastrum stapfianum Extracts and Fractions
4.3. HeLa Cell Culture
4.4. Cell Viability by MTT Assay (Mitochondrial Activity)
4.5. Transfection and Luciferase Gene Reporter Assay
4.6. Acetylcholinesterase Inhibition
4.7. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Scavenging Assay
4.8. Fosfomolybdenium Reduction Assay
4.9. GC-MS Analysis
4.10. HPLC-DAD-UV Analysis
4.11. Statistical and Data Analysis
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Peak | %TIC | RT | Compound | Mass Fragmentation (Relative Intensity) | Reference |
---|---|---|---|---|---|
1 | 0.8 | 34.9 | Lycorine | 226 (100), 227 (59), 250 (19), 27 (187), 286 (13), 147 (10), 228 (9), 119 (9), 248 (9) | [28] |
2 | 0.6 | 35.0 | Non-identified (NI-1) | 343 (100), 341 (71), 344 (36), 266 (33), 40 (29), 252 (28), 196 (21), 282 (19), 310 (19), 283 (18) | - |
3 | 94.6 | 38.0 | 7-Demethoxy-9-O-methylhostasine | 125 (100), 96 (21), 309 (7), 311 (5), 94 (5), 126 (4), 123 (4), 124 (3), 193 (3), 82 (3) | [29] |
4 | 1.3 | 38.4 | Non-identified (NI-2) | 311 (100), 40 (27), 310 (22), 294 (13), 309 (13), 312 (13), 296 (11), 251 (11), 208 (10), 268 (9) | [30] |
5 | 2.7 | 43.0 | Non-identified (NI-3) | 297 (100), 254 (39), 296 (26), 252 (23), 148 (20), 298 (16), 77 (11), 295 (10), 236 (9), 196 (9) | - |
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Gomes-Copeland, K.K.P.; Meireles, C.G.; Gomes, J.V.D.; Torres, A.G.; Sinoti, S.B.P.; Fonseca-Bazzo, Y.M.; Magalhães, P.d.O.; Fagg, C.W.; Simeoni, L.A.; Silveira, D. Hippeastrum stapfianum (Kraenzl.) R.S.Oliveira & Dutilh (Amaryllidaceae) Ethanol Extract Activity on Acetylcholinesterase and PPAR-α/γ Receptors. Plants 2022, 11, 3179. https://doi.org/10.3390/plants11223179
Gomes-Copeland KKP, Meireles CG, Gomes JVD, Torres AG, Sinoti SBP, Fonseca-Bazzo YM, Magalhães PdO, Fagg CW, Simeoni LA, Silveira D. Hippeastrum stapfianum (Kraenzl.) R.S.Oliveira & Dutilh (Amaryllidaceae) Ethanol Extract Activity on Acetylcholinesterase and PPAR-α/γ Receptors. Plants. 2022; 11(22):3179. https://doi.org/10.3390/plants11223179
Chicago/Turabian StyleGomes-Copeland, Kicia Karinne Pereira, Cinthia Gabriel Meireles, João Victor Dutra Gomes, Amanda Gomes Torres, Simone Batista Pires Sinoti, Yris Maria Fonseca-Bazzo, Pérola de Oliveira Magalhães, Christopher William Fagg, Luiz Alberto Simeoni, and Dâmaris Silveira. 2022. "Hippeastrum stapfianum (Kraenzl.) R.S.Oliveira & Dutilh (Amaryllidaceae) Ethanol Extract Activity on Acetylcholinesterase and PPAR-α/γ Receptors" Plants 11, no. 22: 3179. https://doi.org/10.3390/plants11223179