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Volume 11
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10.3390/plants11172259
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Article

CRISPR-Cas9 Gene Editing of the Sal1 Gene Family in Wheat

by
Toni Mohr
1,
James Horstman
1,
Yong Q. Gu
1,
Nagwa I. Elarabi
2,
Naglaa A. Abdallah
2 and
Roger Thilmony
1,*
1
USDA-ARS, Crop Improvement and Genetics Unit, Albany, CA 94710, USA
2
Department of Genetics, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
*
Author to whom correspondence should be addressed.
Plants 2022, 11(17), 2259; https://doi.org/10.3390/plants11172259
Submission received: 5 August 2022 / Revised: 24 August 2022 / Accepted: 26 August 2022 / Published: 30 August 2022
(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
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Abstract

The highly conserved Sal1 encodes a bifunctional enzyme with inositol polyphosphate-1-phosphatase and 3′ (2′), 5′-bisphosphate nucleotidase activity and has been shown to alter abiotic stress tolerance in plants when disrupted. Precise gene editing techniques were used to generate Sal1 mutants in hexaploid bread wheat. The CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats) Cas9 system with three guide RNAs (gRNAs) was used to inactivate six Sal1 homologous genes within the Bobwhite wheat genome. The resulting mutant wheat plants with all their Sal1 genes disabled had slimmer stems, had a modest reduction in biomass and senesced more slowly in water limiting conditions, but did not exhibit improved yield under drought conditions. Our results show that multiplexed gRNAs enabled effective targeted gene editing of the Sal1 gene family in hexaploid wheat. These Sal1 mutant wheat plants will be a resource for further research studying the function of this gene family in wheat.
Keywords: CRISPR; wheat; Sal1; drought tolerance; Triticum aestivum; gene editing CRISPR; wheat; Sal1; drought tolerance; Triticum aestivum; gene editing

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MDPI and ACS Style

Mohr, T.; Horstman, J.; Gu, Y.Q.; Elarabi, N.I.; Abdallah, N.A.; Thilmony, R. CRISPR-Cas9 Gene Editing of the Sal1 Gene Family in Wheat. Plants 2022, 11, 2259. https://doi.org/10.3390/plants11172259

AMA Style

Mohr T, Horstman J, Gu YQ, Elarabi NI, Abdallah NA, Thilmony R. CRISPR-Cas9 Gene Editing of the Sal1 Gene Family in Wheat. Plants. 2022; 11(17):2259. https://doi.org/10.3390/plants11172259

Chicago/Turabian Style

Mohr, Toni, James Horstman, Yong Q. Gu, Nagwa I. Elarabi, Naglaa A. Abdallah, and Roger Thilmony. 2022. "CRISPR-Cas9 Gene Editing of the Sal1 Gene Family in Wheat" Plants 11, no. 17: 2259. https://doi.org/10.3390/plants11172259

APA Style

Mohr, T., Horstman, J., Gu, Y. Q., Elarabi, N. I., Abdallah, N. A., & Thilmony, R. (2022). CRISPR-Cas9 Gene Editing of the Sal1 Gene Family in Wheat. Plants, 11(17), 2259. https://doi.org/10.3390/plants11172259

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