Brachypodium Antifreeze Protein Gene Products Inhibit Ice Recrystallisation, Attenuate Ice Nucleation, and Reduce Immune Response
Abstract
:1. Introduction
2. Results
2.1. AFP-Mediated Attenuation of INP-Induced Freezing and INP Models
2.2. LRR-Mediated Attenuation of the Host Immune Response and Modelling
3. Discussion
4. Materials and Methods
4.1. Protein Modelling and Interaction Predictions
4.2. Construction of LRR Plasmids for Arabidopsis
4.3. Transient Expression in Arabidopsis
4.4. Plant Material and Growth Conditions
4.5. Preparation of Extracts and Apoplast Samples for AFP Activity
4.6. Ice Nucleation Assay
4.7. Immune Response Attenuation ROS Burst Assay
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Juurakko, C.L.; diCenzo, G.C.; Walker, V.K. Brachypodium Antifreeze Protein Gene Products Inhibit Ice Recrystallisation, Attenuate Ice Nucleation, and Reduce Immune Response. Plants 2022, 11, 1475. https://doi.org/10.3390/plants11111475
Juurakko CL, diCenzo GC, Walker VK. Brachypodium Antifreeze Protein Gene Products Inhibit Ice Recrystallisation, Attenuate Ice Nucleation, and Reduce Immune Response. Plants. 2022; 11(11):1475. https://doi.org/10.3390/plants11111475
Chicago/Turabian StyleJuurakko, Collin L., George C. diCenzo, and Virginia K. Walker. 2022. "Brachypodium Antifreeze Protein Gene Products Inhibit Ice Recrystallisation, Attenuate Ice Nucleation, and Reduce Immune Response" Plants 11, no. 11: 1475. https://doi.org/10.3390/plants11111475