A Novel High-Affinity Potassium Transporter IbHKT-like Gene Enhances Low–Potassium Tolerance in Transgenic Roots of Sweet Potato (Ipomoea batatas (L.) Lam.)
Abstract
:1. Introduction
2. Results
2.1. Identification of IbHKT-like Gene from Sweet Potato
2.2. Generation of IbHKT-like Gene Overexpressing Roots in Sweet Potato
2.3. Protection of Cell Ultrastructure in IbHKT-like Gene Overexpressing Roots
2.4. Increase in K+ Absorption in IbHKT-like Gene Overexpressing Roots
2.5. Increased Potassium Content in Plants with IbHKT-like Gene Overexpressing Roots
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Gene Expression Analysis and Phylogenetic Analysis
4.3. Vector Construction and Agrobacterium Rhizogenes-Mediated Transformation
4.4. Observation of Ultrastructure of Root Tip
4.5. Measurement of K+ Fluxes
4.6. Determination of Total Potassium Content
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jiang, W.; Jin, R.; Wang, D.; Yang, Y.; Zhao, P.; Liu, M.; Zhang, A.; Tang, Z. A Novel High-Affinity Potassium Transporter IbHKT-like Gene Enhances Low–Potassium Tolerance in Transgenic Roots of Sweet Potato (Ipomoea batatas (L.) Lam.). Plants 2022, 11, 1389. https://doi.org/10.3390/plants11111389
Jiang W, Jin R, Wang D, Yang Y, Zhao P, Liu M, Zhang A, Tang Z. A Novel High-Affinity Potassium Transporter IbHKT-like Gene Enhances Low–Potassium Tolerance in Transgenic Roots of Sweet Potato (Ipomoea batatas (L.) Lam.). Plants. 2022; 11(11):1389. https://doi.org/10.3390/plants11111389
Chicago/Turabian StyleJiang, Wei, Rong Jin, Danfeng Wang, Yufeng Yang, Peng Zhao, Ming Liu, Aijun Zhang, and Zhonghou Tang. 2022. "A Novel High-Affinity Potassium Transporter IbHKT-like Gene Enhances Low–Potassium Tolerance in Transgenic Roots of Sweet Potato (Ipomoea batatas (L.) Lam.)" Plants 11, no. 11: 1389. https://doi.org/10.3390/plants11111389