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An Argon-Ion-Induced Pale Green Mutant of Arabidopsis Exhibiting Rapid Disassembly of Mesophyll Chloroplast Grana
 
 
Article

Arabidopsis EGY1 Is Critical for Chloroplast Development in Leaf Epidermal Guard Cells

1
Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Kioicho, Chiyoda, Tokyo 102-8554, Japan
2
Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Okinawa 903-0213, Japan
3
Faculty of Agriculture, Kobe University, Nada, Kobe 657-8501, Japan
4
RIKEN Nishina Center, Wako, Saitama 351-0198, Japan
5
Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Eiheiji, Fukui 910-1195, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Mitsumasa Hanaoka
Plants 2021, 10(6), 1254; https://doi.org/10.3390/plants10061254
Received: 6 May 2021 / Revised: 14 June 2021 / Accepted: 17 June 2021 / Published: 21 June 2021
(This article belongs to the Special Issue Molecular Biology of Plastids)
In Arabidopsis thaliana, the Ethylene-dependent Gravitropism-deficient and Yellow-green 1 (EGY1) gene encodes a thylakoid membrane-localized protease involved in chloroplast development in leaf mesophyll cells. Recently, EGY1 was also found to be crucial for the maintenance of grana in mesophyll chloroplasts. To further explore the function of EGY1 in leaf tissues, we examined the phenotype of chloroplasts in the leaf epidermal guard cells and pavement cells of two 40Ar17+ irradiation-derived mutants, Ar50-33-pg1 and egy1-4. Fluorescence microscopy revealed that fully expanded leaves of both egy1 mutants showed severe chlorophyll deficiency in both epidermal cell types. Guard cells in the egy1 mutant exhibited permanent defects in chloroplast formation during leaf expansion. Labeling of plastids with CaMV35S or Protodermal Factor1 (PDF1) promoter-driven stroma-targeted fluorescent proteins revealed that egy1 guard cells contained the normal number of plastids, but with moderately reduced size, compared with wild-type guard cells. Transmission electron microscopy further revealed that the development of thylakoids was impaired in the plastids of egy1 mutant guard mother cells, guard cells, and pavement cells. Collectively, these observations demonstrate that EGY1 is involved in chloroplast formation in the leaf epidermis and is particularly critical for chloroplast differentiation in guard cells. View Full-Text
Keywords: Arabidopsis thaliana; At5g35220; epidermal plastid; guard cell; pavement cell; thylakoid Arabidopsis thaliana; At5g35220; epidermal plastid; guard cell; pavement cell; thylakoid
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MDPI and ACS Style

Sanjaya, A.; Muramatsu, R.; Sato, S.; Suzuki, M.; Sasaki, S.; Ishikawa, H.; Fujii, Y.; Asano, M.; Itoh, R.D.; Kanamaru, K.; Ohbu, S.; Abe, T.; Kazama, Y.; Fujiwara, M.T. Arabidopsis EGY1 Is Critical for Chloroplast Development in Leaf Epidermal Guard Cells. Plants 2021, 10, 1254. https://doi.org/10.3390/plants10061254

AMA Style

Sanjaya A, Muramatsu R, Sato S, Suzuki M, Sasaki S, Ishikawa H, Fujii Y, Asano M, Itoh RD, Kanamaru K, Ohbu S, Abe T, Kazama Y, Fujiwara MT. Arabidopsis EGY1 Is Critical for Chloroplast Development in Leaf Epidermal Guard Cells. Plants. 2021; 10(6):1254. https://doi.org/10.3390/plants10061254

Chicago/Turabian Style

Sanjaya, Alvin, Ryohsuke Muramatsu, Shiho Sato, Mao Suzuki, Shun Sasaki, Hiroki Ishikawa, Yuki Fujii, Makoto Asano, Ryuuichi D. Itoh, Kengo Kanamaru, Sumie Ohbu, Tomoko Abe, Yusuke Kazama, and Makoto T. Fujiwara. 2021. "Arabidopsis EGY1 Is Critical for Chloroplast Development in Leaf Epidermal Guard Cells" Plants 10, no. 6: 1254. https://doi.org/10.3390/plants10061254

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