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Article

Pratylenchus vovlasi sp. Nov. (Nematoda: Pratylenchidae) on Raspberries in North Italy with a Morphometrical and Molecular Characterization

1
Consiglio Nazionale delle Ricerche (CNR), Istituto per la Protezione Sostenibile delle Piante (IPSP), v. G. Amendola, 122/D, 70126 Bari, Italy
2
Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain
3
Departamento de Biología Animal, Biología Vegetal y Ecología, Campus Las Lagunillas, Universidad de Jaén, 23071 Jaén, Spain
4
Regione Piemonte-Settore Fitosanitario e Servizi Tecnico-Scientifici, 10144 Torino, Italy
*
Author to whom correspondence should be addressed.
The ZooBank Life Science Identifier (LSID) for this publication is as follows: http://zoobank.org/urn:lsid:zoobank.org:act:3B91A2A8-B809-47BA-A822-CF380EBC0245; http://zoobank.org/NomenclaturalActs/6B50797F-912D-4117-9B32-48C8BDC4CF01.
Academic Editors: Carla Maleita, Isabel Abrantes and Ivânia Esteves
Plants 2021, 10(6), 1068; https://doi.org/10.3390/plants10061068
Received: 15 April 2021 / Revised: 17 May 2021 / Accepted: 21 May 2021 / Published: 26 May 2021
(This article belongs to the Special Issue Plant Parasitic Nematodes)
Root-lesion nematode species rank third only to root-knot and cyst nematodes as having the greatest economic impact on crops worldwide. A survey of plant-parasitic nematodes associated with decaying raspberries (Rubus sp.) in northern Italy revealed that root-lesion nematodes were the most frequently occurring species among other phytonematodes. Several Pratylenchus species have been associated with Rubus sp. in Canada (Quebec, British Columbia) and USA (North Carolina, Maryland, New Jersey) including P. penetrans and P. crenatus. In the roots and rhizosphere of symptomatic raspberries, nematodes of two Pratylenchus spp. were detected. Detailed morphometrics of the two root-lesion nematode isolates were consistent with Pratylenchus crenatus and with an undescribed Pratylenchus species. The extracted nematodes were observed and measured as live and fixed materials and subsequently identified by integrative taxonomy (morphometrically and molecularly). The latter species is described herein as Pratylenchus vovlasi sp. nov., resulting morphometrically closest to P. mediterraneus and phylogenetically to P. pratensis. The molecular identification of Pratylenchus vovlasi sp. nov. was carried out by sequencing the ITS region, D2-D3 expansion domains of the 28S rRNA gene and a partial region of the nuclear hsp90 gene. ITS-RFLP and sequence analyses revealed that Pratylenchus vovlasi sp. nov. had species-specific restriction profiles with no corresponding sequences present in the database. The phylogenetic relationships with ITS and D2-D3 sequences placed the Pratylenchus vovlasi sp. nov. in a clade with P. pratensis and P. pseudopratensis. This research confirms the occurrence of cryptic biodiversity within the genus Pratylenchus as well as the need for an integrative approach to the identification of Pratylenchus species. View Full-Text
Keywords: Bayesian inference; D2-D3 expansion domains of the 28S rRNA gene; hsp90 gene; integrative taxonomy; ITS Bayesian inference; D2-D3 expansion domains of the 28S rRNA gene; hsp90 gene; integrative taxonomy; ITS
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MDPI and ACS Style

Troccoli, A.; Fanelli, E.; Castillo, P.; Liébanas, G.; Cotroneo, A.; De Luca, F. Pratylenchus vovlasi sp. Nov. (Nematoda: Pratylenchidae) on Raspberries in North Italy with a Morphometrical and Molecular Characterization. Plants 2021, 10, 1068. https://doi.org/10.3390/plants10061068

AMA Style

Troccoli A, Fanelli E, Castillo P, Liébanas G, Cotroneo A, De Luca F. Pratylenchus vovlasi sp. Nov. (Nematoda: Pratylenchidae) on Raspberries in North Italy with a Morphometrical and Molecular Characterization. Plants. 2021; 10(6):1068. https://doi.org/10.3390/plants10061068

Chicago/Turabian Style

Troccoli, Alberto, Elena Fanelli, Pablo Castillo, Gracia Liébanas, Alba Cotroneo, and Francesca De Luca. 2021. "Pratylenchus vovlasi sp. Nov. (Nematoda: Pratylenchidae) on Raspberries in North Italy with a Morphometrical and Molecular Characterization" Plants 10, no. 6: 1068. https://doi.org/10.3390/plants10061068

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