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Article

New Phenolic Compounds in Posidonia oceanica Seagrass: A Comprehensive Array Using High Resolution Mass Spectrometry

1
Department of Biology and Geology, International Campus of Excellence in Marine Science (CEIMAR), University of Almeria, E-04120 Almeria, Spain
2
Department of Chemistry and Physics, Research Centre for Mediterranean Intensive Agrosystems and Agri-Food Biotechnology (CIAIMBITAL), Agrifood Campus of International Excellence ceiA3, University of Almería, E-04120 Almeria, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Suresh Awale
Plants 2021, 10(5), 864; https://doi.org/10.3390/plants10050864
Received: 6 April 2021 / Revised: 22 April 2021 / Accepted: 23 April 2021 / Published: 25 April 2021
(This article belongs to the Special Issue Structural and Functional Analysis of Extracts in Plants II)
The studies on the Posidonia oceanica Delile (P. oceanica) phenolic composition have been focused on the foliar tissues and have often neglected the phenolic compounds in rhizomes or roots alike. With the current improvements in high resolution mass spectrometry (HRMS) analyzers, such as the Orbitrap MS, there is a new opportunity to more deeply study P. oceanica. One of the benefits is the possibility of conducting an exhaustive phenolic monitoring, which is crucial in the search for new stressor-specific biomarkers of coastal deterioration. For this purpose, the different tissues (leaf, rhizome, and root) of P. oceanica seagrass from several marine sampling areas were analyzed through target, suspected, and non-target screenings. This paper brings a fast and tissues-specific extraction, as well as a detection method of phenolic compounds applying for the first time the potential of HRMS (Exactive Orbitrap) in P. oceanica samples. As a result, 42 phenolic compounds were satisfactorily detected, of which, to our knowledge, 24 were not previously reported in P. oceanica, such as naringenin, naringenin chalcone and pinocembrin, among others. Information here reported could be used for the evaluation of new stressor-specific biomarkers of coastal deterioration in the Mediterranean waters. Furthermore, the followed extraction and analytical method could be considered as a reference protocol in other studies on marine seagrasses due to the exhaustive search and satisfactory results. View Full-Text
Keywords: Posidonia oceanica; leaf; rhizome; root; UHPLC-Orbitrap MS; phenolic compounds Posidonia oceanica; leaf; rhizome; root; UHPLC-Orbitrap MS; phenolic compounds
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MDPI and ACS Style

Astudillo-Pascual, M.; Domínguez, I.; Aguilera, P.A.; Garrido Frenich, A. New Phenolic Compounds in Posidonia oceanica Seagrass: A Comprehensive Array Using High Resolution Mass Spectrometry. Plants 2021, 10, 864. https://doi.org/10.3390/plants10050864

AMA Style

Astudillo-Pascual M, Domínguez I, Aguilera PA, Garrido Frenich A. New Phenolic Compounds in Posidonia oceanica Seagrass: A Comprehensive Array Using High Resolution Mass Spectrometry. Plants. 2021; 10(5):864. https://doi.org/10.3390/plants10050864

Chicago/Turabian Style

Astudillo-Pascual, Marina, Irene Domínguez, Pedro A. Aguilera, and Antonia Garrido Frenich. 2021. "New Phenolic Compounds in Posidonia oceanica Seagrass: A Comprehensive Array Using High Resolution Mass Spectrometry" Plants 10, no. 5: 864. https://doi.org/10.3390/plants10050864

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