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Article

Marker-Assisted Introgression and Stacking of Major QTLs Controlling Grain Number (Gn1a) and Number of Primary Branching (WFP) to NERICA Cultivars

1
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
2
Novel Gene Resources Laboratory, Plant Breeding Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines
3
Graduate School, University of the Philippines Los Banos, College, Laguna 4031, Philippines
4
Current address: Department of Plant and Soil Science, College of Agricultural Sciences and Natural Resources, Texas Tech University, Lubbock, TX 79409, USA
5
Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan
6
Applied Social System Institute of Asia, Nagoya University, Nagoya 464-8601, Japan
7
International Center for Research and Education in Agriculture, Nagoya University, Nagoya 464-8601, Japan
*
Author to whom correspondence should be addressed.
These two authors contributed equally.
Academic Editor: Yong-Gu Cho
Plants 2021, 10(5), 844; https://doi.org/10.3390/plants10050844
Received: 18 March 2021 / Revised: 19 April 2021 / Accepted: 19 April 2021 / Published: 22 April 2021
(This article belongs to the Special Issue Genomics, Genetics, and Breeding for Rice Crop Improvement)
The era of the green revolution has significantly improved rice yield productivity. However, with the growing population and decreasing arable land, rice scientists must find new ways to improve rice productivity. Although hundreds of rice yield-related QTLs were already mapped and some of them were cloned, only a few were utilized for actual systematic introgression breeding programs. In this study, the major yield QTLs Grain Number 1a (Gn1a) and Wealthy Farmer’s Panicle (WFP) were introgressed and stacked in selected NERICA cultivars by marker-assisted backcross breeding (MABB). The DNA markers RM3360, RM3452, and RM5493 were used for foreground selection. At BC3F4 and BC3F5 generation, a combination of marker-assisted selection and phenotypic evaluation were carried out to select lines with target alleles and traits. Further, genotyping-by-sequencing (GBS) was conducted to validate the introgression and determine the recurrent parent genome recovery (RPGR) of the selected lines. The Gn1a and/or WFP introgression lines showed significantly higher numbers of spikelets per panicle and primary branching compared to the recurrent parents. In addition, lines with Gn1a and/or WFP alleles were comparatively similar to the recurrent parents (RP) in most yield-related traits. This study demonstrates the success of utilizing yield QTLs and marker-assisted selection to develop and improve rice cultivars. View Full-Text
Keywords: Gn1a; WFP; marker-assisted backcrossing; genotyping-by-sequencing; crop improvement; molecular breeding; NERICA; rice yield Gn1a; WFP; marker-assisted backcrossing; genotyping-by-sequencing; crop improvement; molecular breeding; NERICA; rice yield
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MDPI and ACS Style

Reyes, V.P.; Angeles-Shim, R.B.; Mendioro, M.S.; Manuel, M.C.C.; Lapis, R.S.; Shim, J.; Sunohara, H.; Nishiuchi, S.; Kikuta, M.; Makihara, D.; Jena, K.K.; Ashikari, M.; Doi, K. Marker-Assisted Introgression and Stacking of Major QTLs Controlling Grain Number (Gn1a) and Number of Primary Branching (WFP) to NERICA Cultivars. Plants 2021, 10, 844. https://doi.org/10.3390/plants10050844

AMA Style

Reyes VP, Angeles-Shim RB, Mendioro MS, Manuel MCC, Lapis RS, Shim J, Sunohara H, Nishiuchi S, Kikuta M, Makihara D, Jena KK, Ashikari M, Doi K. Marker-Assisted Introgression and Stacking of Major QTLs Controlling Grain Number (Gn1a) and Number of Primary Branching (WFP) to NERICA Cultivars. Plants. 2021; 10(5):844. https://doi.org/10.3390/plants10050844

Chicago/Turabian Style

Reyes, Vincent P., Rosalyn B. Angeles-Shim, Merlyn S. Mendioro, Ma. Carmina C. Manuel, Ruby S. Lapis, Junghyun Shim, Hidehiko Sunohara, Shunsaku Nishiuchi, Mayumi Kikuta, Daigo Makihara, Kshirod K. Jena, Motoyuki Ashikari, and Kazuyuki Doi. 2021. "Marker-Assisted Introgression and Stacking of Major QTLs Controlling Grain Number (Gn1a) and Number of Primary Branching (WFP) to NERICA Cultivars" Plants 10, no. 5: 844. https://doi.org/10.3390/plants10050844

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